C1orf77 (CHTOP) (AF261137) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C1orf77; FL-SRAG; FOP; pp7704; SRAG; SRAG-3; SRAG-5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF261137, the custom clone sequence may differ by one or more nucleotides ATGGCTGCACAGTCAGCGCCGAAAGTTGTGCTAAAAAGCACCACCAAGATGTCTCTAAATGAGCGCTTTA CTAATATGCTGAAGAACAAACAGCCGACGCCAGTGAATATTCGGGCTTCGATGCAGCAACAACAGCAGCT AGCCAGTGCCAGAAACAGAAGACTGGCCCAGCAGATGGAGAATAGACCCTCTGTCCAGGCAGCATTAAAA CTTAAGCAGAGCTTAAAGCAGCGCCTGGGTAAGAGTAACATCCAGGCACGGTTAGGCCGACCCATAGGGG CCCTGGCCAGGGGAGCAATCGGAGGACGAGGCCTACCCATAATCCAGAGAGGCTTGCCCAGAGGAGGACT ACGTGGGGGACGTGCCACCAGAACCCTACTTAGGGGCGGGATGTCACTCCGAGGTCAAAACCTGCTCCGA GGTGGACGAGCCGTAGCTCCCCGAATGGGCTTAAGAAGAGGTGGTGTTCGAGGTCGTGGAGGTCCTGGGA GAGGGGGCCTAGGGCGTGGAGCTATGGGTCGTGGCGGAATCGGTGGTAGAGGTCGGGGTATGATAGGTCG GGGAAGAGGGGGCTTTGGAGGCCGAGGCCGAGGCCGTGGACGAGGGAGAGGTGCCCTTGCTCGCCCTGTA TTGACCAAGGAGCAGCTGGACAACCAATTGGATGCATATATGTCGAAAACAAAAGGACACCTGGATGCTG AGTTGGATGCCTACATGGCGCAGACAGATCCCGAAACCAATGATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF261137 unedited TTCACCAATATGTAATACGACTCACTATAGGGGCGGCCGCGAATCGGCACGAGGGTTTTG GACACCGAGCAGGAGCTGGCGGCCGCTGCAGACGAAAGGCAGGAAAGGGCAGGCCGGGTG AGCAGACGGATCGGCCGACTAGACAGCCAACCAGCAACAACGAACTGAGCTCGCATACTA CCGCTTACGCATCTAACCAACCGCCCATCTAGCTAACCCGAGCCCCTCCACCGTCAACTC AGGTTCGGCCGGTCCCCGGCCCGCCTGCCGGAGCCGTGGTGGCAGCCCCGGGAGGAGCAC TGGCGTCTGTTTCCTTCGATTCTCGGGATTCGAAGATGGCTGCACAGTCAGCGCCGAAAG TTGTGCTAAAAAGCACCACCAAGATGTCTCTAAATGAGCGCTTTACTAATATGCTGAAGA ACAAACAGCCGACGCCAGTGAATATTCGGGCTTCGATGCAGCAACAACAGCAGCTAGCCA GTGCCAGAAACAGAAGACTGGCCCAGCAGATGGAGAATAGACCCTCTGTCCAGGCAGCAT TAAAACTTAAGCAGAAGAGCTTAAAGCAGCGCCTGGGTAAGAGTAACATCCAGGCACGGT TAGGCCGACCCATAGGGGCCCTGGCCAGGGGAGCAATCGGAGGACGAGGCCTACCATAAT CCAGAGAGGCTTGCCCAAGAGAGGACTACGTGGGGGACGTGCCACCAGAACCCTACTTAG GGGCGGGATGTCACTCCGCAGTCAAAACCTGCTCCGAGGTGGACGAGCCGTAACTCCCCG AATGGGCTTAANAANAAGTGGTGTTTCAGTCCTGGAGTCCTGGGAGAGGGGCCCAGGCGT GGAGCATGGGTCGTGGCGAATCGGGGTTAAGGTCGGTATGAAAGTCCGGACAAGGGCTTT GAAGCCAAGCCAGCCCGGCCAAGAAAGGGCCCTGCTGCCTGTTC
Restriction Sites	NotI-NotI
ACCN	AF261137
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF261137.1, AAG44673.1
RefSeq Size	1293 bp
RefSeq ORF	1293 bp
Locus ID	26097
Gene Summary	This gene encodes a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2015]

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