PDE6 beta (PDE6B) (BC020343) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020343, the custom clone sequence may differ by one or more nucleotides CAGGGGGTTCAGGAGGCGAGGAGGCCAGGGCGTGGGTTGGAGGACGGCTGTGCTGCCTTCTTCCTCCTCC CCAGGCTGCCGTGCTGGGGCCCCTCCCTCTTGGGAACCCCTCCCTCTCCGGAGGCCGTATGGCTTCAGCC TGCTTTCTAGGACTCAGCGTTGGTGCCTGCCCTGCCACCCTGACCCTGACTTGTGCTCAAGCCTGGCACC TGGATCCTGGTTCCTGCTTGGGGCCTGGGGATGGGGTCCCAATAGGCTTATGGGATCACAGGGCCACTGG GCCACCAGCTGCCCCTCCCTTGGCTGGAGTCCTGCCCTGACCTCCGGACTGGAGTTCCCCTCCTCTTCCA GCCCCACGGGGCCAAGACTCGGCCTCCTGGCTGGAGGCTCCACTGTCTGGGTGGGGCTGGTGGTGCCAGG GTCAAATCCTGGGGCCTTCATGCTCAATTGTTGCTGACGAGGCCGCCTGGACAGCTTGCCTGGTCCCCTG TATGTCCACTCTGGATGTCCACAGTGACTCGGGCTCCTCAGCAGCCCACGTCCTCCTCACCATCTCCCAG AACATTGGAGTCAGGGAAACAGAGGCGTGTGGACAGGCCCTGGGCCCCTCCCCTAAGACTCACCCGGCCA TCAGGCGTGCGTGGGCCCGAGTACGGCTGGGACTCTCCCATCAGCACAGACCACACGTCAAAAAATTCCT GGGCAGAGAAGAGGAGGCCCACGGAGTACCGCTCGCAGTTGAGGTAGGCCCGCACCGTGTAGAAGGCCTT GTGGAACTGCCTCTCGATGTCCGTCAGCTCCTCAAACACCTTGTTGGCCGACCACAGCAGCACCTGGAGG GAGGGGCACACCTGTGGGCCTGTGGCCACTCCCACCCTCACGCCGGTCTGACCACAGCAGCAGGGGTTTA ACTCAAGCCTGACACAGGTTTCATCTGAGCTTGTGGGGCCCGGGAGCTGGCTGGCGGTGAGGCCTCGAGG CTGCGGTCGTGTCCCTGATCACTCAGGAGTGGACGAGTGGAAAGTGGAATCTGTCGCTTTGAGCTTGACA GGCAAACATCCGACCAGCCCTTCCTCCGCCCGGCTGCTCCAGAACCACCTGCGGCGAGCAGGGCTTTGAG AACATCGGGCCGTGGTTTCCGCACGTCAGGCCGCCTGTGGCCTCCGCCAAGCGTCTGAGAGTGAAGCATT GCTGACGCACTCCACGAGGTCCTGGCCAGACCCCACTCAGGACATCCCGCTGCACAGGCCCCTGGTTGCT GGGTCAGCGCTGCCACCAGGCTCAGACTCACCCCAGGGCCCTCCTTGGGCCACCTTTCTCTGCCTCCTTG GTCAAGGCCCAGACCCAGGCCCCACCAGGCCCTGCCTCCTCACACTCGCGGCGGCCGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020343 unedited TCACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCCTGCCACCCT GACCCTGACTTGTGCTCAAGCCTGGCACCTGGATCCTGGTTCCTGCTTGGGGCCTGGGGA TGGGGTCCCAATAGGCTTATGGGATCACAGGGCCACTGGGCCACCAGCTGCCCCTCCCTT GGCTGGAGTCCTGCCCTGACCTCCGGACTGGAGTTCCCCTCCTCTTCCAGCCCCACGGGG CCAAGACTCGGCCTCCTGGCTGGAGGCTCCACTGTCTGGGTGGGGCTGGTGGTGCCAGGG TCAAATCCTGGGGCCTTCATGCTCAATTGTTGCTGACGAGGCCGCCTGGACAGCTTGCCT GGTCCCCTGTATGTCCACTCTGGATGTCCACAGTGACTCGGGCTCCTCAGCAGCCCACGT CCTCCTCACCATCTCCCAGAACAGAGGCCCACGGAGTACCGCTCGCAGTTGAGGTAGGCC CGCACCGTGTAGAAGGCCTTGTGGAACTGCCTCTCGATGTCCGTCAGCTCCTCAAACACC TTGTTGGCCGACCACAGCAGCACCTGGAGGGAGGGGCACACCTGTGGGCCTGTGNGCCAC TCCACCCTCACGCCGGTCTGACCACAGCAGCAGGNGTTTAACTCAAGCCTGACACANGTG CGCTTCCTGATCTGACCACCTGCCCAGCCGTGGAGCCTGGCACAGACCCTGATTGGTGGC TCCGTGGCCAGGTTTCATCTGAGCTTGTGGGGCCCCGGAGCCTGCCTGCGGTGAGGCCCT CCAGCTGCNGTCGTGTCCCTGACACTCANGAGTGTACCAGTGGAAAGATGAATCTGTCGC TTTGAGCTTGACAGGNAACATTCGACCAGNCCTTTCTTCGCCCGGNTGTTTCAGAACCAC CTGCGGGAGCAGGGCTTTGAGAACTTCGGCCCGTGTTTCCCCCCGTCAAGCCCCCTGTTG GCTCGC
Restriction Sites	NotI-NotI
ACCN	BC020343
Insert Size	1950 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020343.1
RefSeq Size	1411 bp
RefSeq ORF	1098 bp
Locus ID	5158
Protein Families	Druggable Genome
Gene Summary	Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

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