NDUF3 (NDUFAF3) (BC002873) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2P1; chromosome 3 open reading frame 60; DKFZp564J0123; E3-3; MGC10527; nuclear protein E3-3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC002873, the custom clone sequence may differ by one or more nucleotides ATGGCCACCGCTCTCGCGCTACGTAGCTTGTACCGAGCGCGACCCTCGCTGCGCTGTCCGCCCGTTGAGC TTCCCTGGGCCCCGCGGCGAGGGCATCGGCTCTCGCCGGCGGATGACGAGCTGTATCAGCGGACGCGCAT CTCTCTGCTGCAACGCGAGGCCGCTCAGGCAATGTACATCGACAGCTACAACAGCCGCGGCTTCATGATA AACGGAAACCGCGTGCTCGGCCCCTGCGCTCTGCTCCCGCACTCGGTGGTGCAGTGGAACGTGGGATCCC ACCAGGACATCACCGAAGACAGCTTTTCCCTCTTCTGGTTGCTGGAGCCCCGGATAGAGATCGTGGTGGT GGGGACTGGAGACCGGACCGAGAGGCTGCAGTCCCAGGTGCTTCAAGCCATGAGGCAGCGGGGCATTGCT GTGGAAGTGCAGGACACGCCCAATGCCTGTGCCACCTTCAACTTCCTGTGTCATGAAGGCCGAGTAACTG GAGCTGCTCTCATCCCTCCACCAGGAGGGACTTCACTTACATCTTTGGGCCAAGCTGCTCAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002873 unedited TTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGCGACCAGGTGGGCGG TCGCCCCGCCCCGGGAGCGCGGCTTAATAGCTGAGAGCCCGGGGGCCAGGCCGCGGCTGC GGCCCAGGCAACGCCCTGAGGGTGGCCACGCTGCCAGGTGTTCCACTCCCCCGGGACTAT GGGCAAGGGCCCGGGGCGGGGAGGGCGGCAGGTGCTGACACTGGAGCTGCGCCGGAGGTC GGGGAACTCGGCCTCCTAAGACTGAGGACACTCGCCTGCTGGGCCGGTCGAGCTGTGCGG TGCCCTCCGGGACGCAGGGGGCGCTGCAGCCACGCTGGGTCAGGCTCCGCAGGGCCCTCC CAACCCGGGGACTAACGGCGCCGGTGACGACTTCGCCGCGCGTTGGTCAGCCATGGCCAC CGCTCTCGCGCTACGTAGCTTGTACCGAGCGCGACCCTCGCTGCGCTGTCCGCCCGTTGA GCTTCCCTGGGCCCCGCGGCGAGGGCATCGGCTCTCGCCGGCGGATGACGAGCTGTATCA GCGGACGCGCATCTCTCTGCTGCAACGCGAGGCCGCTCAGGCAATGTACATCGACAGCTA CAACAGCCGCGGCTTCATGATAAACGGAAACCGCGTGCTCGGCCCCTGCGCTCTGCTCCC GCACTCGGTGGTGCAGTGGAACGTGGGATCCCACCAGGACATCACCGAAGACAGCTTTTC CCTCTTCTGGGTGCTGGAGCCCCGGATAGGTACTGGGGGAGGGGGAGGAGACAAGAGTGT TCTGGGCCCCAAAGGGGACCCCACTGCAGCCCTCACAAACTGTAGATAGCCCACCCACCC TCCTAGAGATG
Restriction Sites	NotI-NotI
ACCN	BC002873
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002873.2, AAH02873.1
RefSeq Size	743 bp
RefSeq ORF	555 bp
Locus ID	25915
Domains	DUF598
Gene Summary	This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.