ZNF148 (BC018971) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BERF-1; BFCOL1; CACCC box-binding protein; CLL-associated antigen KW-10; HT-BETA; pHZ-52; ZBP-89; ZFP148; zinc finger protein 148
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC018971, the custom clone sequence may differ by one or more nucleotides ATGCGTTTCATACAGAAGTACCTGCTTCAGAGACATGAGAAGATTCATACTGGTGAAAAACCATTTCGCT GTGATGAATGTGGTGATGAGACAAACCATGCTTCTGCCACATCAACACAGGACTTTCTGGATCAAGTGAC TTCTCAGAAGAAAGCTGAGGCCCAGCCTGTCCACCAAGCTTACCAAATGAGCTCCTTTGAACAGCCCTTC CGTGCTCCCTATCATGGATCAAGAGCTGGAATAGCTACTCAATTTAGCACTGCCAATGGACAGGTGAACC TTCGGGGACCAGGGACAAGTGCTGAATTTTCAGAATTTCCCTTGGTGAATGTAAATGATAATAGAGCTGG GATGACATCTTCACCTGATGCCACAACTGGCCAGACTTTTGGCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC018971 unedited GTGCCCATTTGTATACGACTCCTATAGGCGGCCGCGATTCGCCGAGGAAAGCGCAGGGTG GGAGCTGTTGCCGAAGCTGCCACAGCAAAAGTTCTCCCCCCTCCCCCCTTCCCCTCCTCT CAAGGCCCCTAGAAAGGTTGGAGCTGCCGCCGCCTGCAGTCGGTGACCGCGCGACTCGGC GCCCGCCCGCGGATAGAGGGAGGAATCAGCAGCTTGGAAATTCAAGCACGTGATCTGGCG GGATGGGCGTTTGCCTAACGTATTTAATGGAGGAATCGGATGGCATAAGTGATTAAGGTG GTATTGAGGATTTCTGAAGCCTATGAAAGGTAGAAACTCAACCATGATTTCTTTTTCAAC TCTACAGCATTCCTTTCCTTGAAGTCTTCGTTTTTACCTTAGTCTCGGGCAGTTATACTT AAGCATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGA AATGCAGTCTTCCAGGACAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCTACTGTGTC TGGAGAGCTACAGGATTCAGTACTTCAAGATCGAAGTATGCCTCACCAGGAGATCCTTGC TGCAGATGAAGTGTTACAAGAAAGTGAAATGAGACAACAGGATATGATATCACATGATGA ACTCATGGTCCATGANGAGACAGTGAAAAATGATGAAGAGCAGATGGAAACACATGANAG ACTTCCTCAAGGACTACAGTATGCACTTAATGTCCCTATNAGCGNTAAGCANGAAATTAC TTTTACTGATGTATCTGAGCAACTGATGAGAGACAAAAACCAATTAGAGAGCCAGTAGAC TTACAGAAAAAAGAGAAAGCGAAACCACGTCCTCCCGCAAAATCCTTACAATAATGAGGA TGGATCACTTGGTTTGGAAACCCCTAATCTTACTTTG
Restriction Sites	NotI-NotI
ACCN	BC018971
Insert Size	4600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018971.1
RefSeq Size	1034 bp
RefSeq ORF	832 bp
Locus ID	7707
Domains	zf-C2H2
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]

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