SUGT1 (AF068289) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF068289, the custom clone sequence may differ by one or more nucleotides ATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGACTAAGGCTTTGGAACAGAAACCAGATGATGCAC AGTATTATTGTCAAAGAGCTTATTGTCACATTCTTCTTGGGAATTACTGTGTTGCTGTTGCTGATGCAAA GAAGTCTCTAGAACTCAATCCAAATAATTCCACTGCTATGCTGAGAAAAGGAATATGTGAATACCATGAA AAAAACTATGCTGCTGCCCTAGAAACTTTTACAGAAGGACAAAAATTAGATATAGAGACGGGGTTTCATC GTGTTGGCCAGGCTGGTCTCCAACTCTTGACCTCAAGTGATCCACCTGCCTTGGACTCCCAAAGTGCTGG GATTACAGGTGCAGATGCTAATTTCAGTGTCTGGATTAAAAGGTGTCAAGAAGCTCAGAATGGCTCAGAA TCTGAAGTGTGGACTCATCAGTCAAAAATCAAGTATGACTGGTATCAAACAGAATCTCAAGTAGTCATTA CAATTTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF068289 unedited GTTGCACCATTATGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGCG CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGG TGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGTGTGTTCTCGGCTCTGCA GCCCCCGCTTCCCAGGTTCAAGTAACTGCCTCAGCCTTCTGAGTAGCTGGGATTACAGGT GTGCACCACCACGCCCAGCTCAAAGAAAATGAACGGCACCCTGGACCACCCAGACCAACC AGATCTTGATGCTATCAAGATGTTTGTGGGCCAGGTTCCAAGGACCTGGTCTGAAAAGGA CTTGCGGGAACTCTTCGAACAGTATGGTGCTGTGTATGAAATCAACGTCCTAAGGGATAG GAGCCAAAACCCGCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTTACACCCGTAAAGC TGCATTAGAAGCTCAGAATGCTCTTCACAACATGAAAGTCCTCCCAGGGATGCATCACCC TATACAGATGAAACCTGCTGACAGTGAGAAGAACAATGCAGTGGAAGACAGGAAGCTGTT TATTGGTATGATTTCCAAGAAGTGCACTGAAAATGACATCCGAGTCATGTTCTNCTTCGT TTGGACAGATTGAAAGATGCCGGATATTGCCGGGACCTGATGGCCTGAGCCGAGGTGTGC ATTTNGACTTTTACACAAGAGCCATGGACAGACGCTATCAAGGCATGCACCAGCCAGACA TGGAAGTTGCTCATACCCAT
Restriction Sites	NotI-NotI
ACCN	AF068289
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF068289.1, AAF65508.1
RefSeq Size	1089 bp
RefSeq ORF	1089 bp
Locus ID	10910
Domains	TPR
Protein Pathways	NOD-like receptor signaling pathway
Gene Summary	This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. This protein interacts with heat shock protein 90. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene have been defined on several different chromosomes. [provided by RefSeq, Mar 2016]

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