Complement factor B (CFB) (AH001502) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AHUS4; ARMD14; BF; BFD; CFAB; CFBD; FB; FBI12; GBG; H2-Bf; PBF2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AH001502, the custom clone sequence may differ by one or more nucleotides CAAATGATCGATGAAAGCCAGTCTCTGAGTCTCTGTGGCATGGTTTGGGAACACAGGAAGGGTACCGATT ACCACAAGCAACCATGGCAGGCCAAGATCTCAGTCATTCGCCCTTCAAAGGGCCACGAGAGCTGTATGGG GGCTGTGGTGTCTGAGTACTTTGTGCTGACAGCAGCACATTGTTTCACTGTGGATGACAAGGAACACTCA ACTAAGGTCAGCGTAGGAGGGGAGAAGCGGNNNNNNNNNATAGAAGTAGTCCTATTTCACCCCAACTACA ACATTAATGGGAAAAAAGAAGCAGGAATTCCCGAATTTTATGACTATGACGTTGCCCTGATCAAGCTCAA GAATAAGCTGAAATATGGCCAGACCATCAGGCCCATTCTGAAGGAGAAACTCCAAGATGAGGATTTGGGT TTTCATTAAGGGGTTTTCTGCTGAACAGGGGCGTGGGATTGAATTAAAACAGCTGCGACAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AH001502 unedited GGGGTTTANNATTAGNATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAAAACC TGTTGCTCATTGTCTTTCTTGGAAGCCAAAGCATTGATGTTCAAAGTTAAGGATATGGAA AACCTGGAAGATGTTTTCTACCAAATGATCGATGAAAGCCAGTCTCTGAGTCTCTGTGGC ATGGTTTGGGAACACAGGAAGGGTACCGATTACCACAAGCAACCATGGCAGGCCAAGATC TCAGTCATTCGCCCTTCAAAGGGACACGAGAGCTGTATGGGGGCTGTGGTGTCTGAGTAC TTTGTGCTGACAGCAGCACATTGTTTCACTGTGGATGACAAGGAACACTCAATCAAGGTC AGCGTAGGAGGGGAGAAGCGGGACCTGGAGATAGAAGTAGTCCTATTTCACCCCAACTAC AACATTAATGGGAAAAAAGAAGCAGGAATTCCTGAATTTTATGACTATGACGTTGCCCTG ATCAAGCTCAAGAATAAGCTGAAATATGGCCAGACTATCAGGCCCATTTGTCTCCCCTGC ACCGAGGGAACAACTCGAGCTTTGAGGCTTCCTCCAACTACCACTTGCCAGCAACAAAAG GAAGAGCTGCTCCCTGCACAGGATATCAAAGCTCTGTTTGTGTCTGAGGAGGAGAAAAAG CTGACTCGGAAGGAGGTCTACATCAAGAATGGGGATAAGAAAGGCAGCTGTGAGAGAGAT GCTCAATATGCCCCAGGCTATGACAAAGTCAAGGACATCTCAGAGGTGGTCACCCCTCGG TTCCTTTGTACTGGAGGAGTGAGTCCCTATGCTGACCCCAATACTGCAAGGTGATTCTGC GGCCCTTGTAGTCACAGAGAGTCGTTATTCAGTTGTGTATCACTGGGAGTAGTGATGTCT CAAAACAGAGCGCAAGCAGTACTGTACGCGATATACT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AH001502 unedited GTTCAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGTTG TCGCAGCTGTTTTAATTCAATCCCACGCCCCTGTCCAGCAGGAAACCCCTTATAGAAAAC CCAAATCCTCATCTTGGAGTTTCTCCTTCAGCCAGGGCAGCACTTGAAAGAGGTTGATGT GAAAGTCTCGGGCGTGAGCAGGTACCTGCTTTTGCCGCTTCTGGTTTTTGCAGACATCCA CTACTCCCCAGCTGATTACACCAACTTGAATGAAACGACTTCTCTTGTGAACTATCAAGG GGCCGCCAGAATCACCTCTGCAAGTATTGGGGTCAGCATAGGGACTCACTCCTCCAGTAC AAAGGAACCGAGGGGTGACCACCTCTGAGATGTCCTTGACTTTGTCATAGCCTGGGGCAT ATTGAGCATCTCTCTCACAGCTGCCTTTCTTATCCCCATTCTTGATGTAGACCTCCTTCC GAGTCAGCTTTTTCTCCTCCTCAGACACAAACAGAGCTTTGATATCCTGTGCAGGGAGCA GCTCTTCCTTTTGTTGCTGGCAAGTGGTAGTTGGAGGAAGCCTCAAAGCTCGAGTTGTTC CCTCGGTGCAGGGGAGACAAATGGGCCTGATAGTCTGGCCATATTTCAGCTTATTCTTGA GCTTGATCAGGGCAACGTCATAGTCATAAAATTCAGGAATTCCTGCTTCTTTTTTCCCAT TAATGTTGTAGTTGGGGTGAAATANGACTACTTCTATCTNCAGGTCCCGCTTCTCCCTCC TACGCTGACCTTGATTGAGTGGTCCTTGTCATCCACAGTGGACCAATGTGCTGCTGTCAG CCCAAGTACTCAGAACCACAGCCCCCATACGCTCTCGTGTCCCTTTGAGGGCGATGACTG AGATCTTGGCCTGCCATGGNTGCTGTGGAAAN
Restriction Sites	NotI-NotI
ACCN	AH001502
Insert Size	1150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AH001502.1, AAA36219.1
RefSeq Size	482 bp
RefSeq ORF	482 bp
Locus ID	629
Protein Families	Druggable Genome, Protease, Secreted Protein
Protein Pathways	Complement and coagulation cascades
Gene Summary	This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]

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