FBLIM1 (AL834400) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL834400, the custom clone sequence may differ by one or more nucleotides CTCTGACAAGACTTGTGTTAGCAGATTGATGTTCAGGCTTCAGTATCCTCCTGACTTAACAAACAATTAC ATTATTCATTCAGGAAACCTTATTCAGCACCCACTAGGTGCTCAGGTTCTCTGCAAAGGACACAGAAATG AATGATACGTGTTCCTTGCATATGGCACTGTCTAGAGGGGACATACACAGCTGAAGAAGGGAAGGGCAGT GATGGGGTTATGGAGGAAGTATGGCAGGGAAAGCAGGAGGGTGATGCTGGAAGACAAATGGTAGATCCCA GGCGGACTGGAAAGAATTGCAATAGGGGGAGCCGGGGAGTGCAGTCTGTGAGAGGCTGGAAATGTGAAAG CCAGGAGCTCTCAGGGATTTGGGTTGCCTCCTGCACAGTCAAGGAGAGTGTCACTCAGGCTAGTCATAGA TGGCATACCTACAGGCTTGGGCTGATTTTAGATAGTTGTTCAGAGCAGTCAGGCAGGCGCCAGAGGCACA AGCCCTGTGAGAGGTGTGTTTGGAATCACTTGGTATCCAAATCACATGTCATCCCTAGGAGCAAGGAGAC CGGTTGGATTTGGGGCTGGTCTGGAATGAAGAAGCATCTCTCAGCTTCCGAAGGCTGCATGGCTCACGGC AGTATCTCAATCATGCCCTGGCCGTTTGCACATTTGGTCCTGTTGGTAGTTACCTCTCTGGTGACTGGCA AAGTCAGCAAGGATATTGGGGTGGAGCACCCTGGCTAAGCACCCTCTTGATTATAGAGCTATGGAGTAGA AGACAGATGGAGAATGAGAGGCCTGTGAGAACAATCAGTCAGTTGCCATCTTTCAGAGCTGCTCGAGTTC AAAAGTGTGTCATACAGGGTATTTTACCATTGCTACCTTTAAGGGATTCAGGTGGGAACCCTAGGCTGTG GCTTCACATAATGGAACTTGGGTGCCATCCTACCCTGTGATGTTGAGCTGGCCAGCACTGGTGTAGGAAC CTCAAGGACTCTGTGCTTCTCTGGTTTGGGGCCTAGGATAAACACAGGCCCTGCTTGCCCCTTGGTTCTA GGACACATCTTCCCATGCCAGCAAAGTTAATGAACCAGTCTACAAGATAACTGTTGAAAGAATTCTTAGA GAAACAACCCACAGGAGGGGAGCCATGTCAGAGCCCAAGAAAACCATCCCATCCTGAGCTCTGCCTTCTG TGCTGCTCTCTGCATCCTATTCTCTGTCGGAACAGGACCCCACTGTGTCCTTGCTCTTCTAGGCAGAGTT GTGGGAGAGTGCTAAATGCTTTTGAGTGGGGCAGCCTGTGGAATGGGATCTCATACTGGCTCCTTAGACC TTGGGCCCATGAACTCAGTATGGAGCAGGACCTGCGATGTTCTGATGGATTATACCCACAGTGTATTTTG GCACATCTGCCAAAAGCTACAAACAACCCCCAACAACTACACACTATATCTTGTGAGAAGTGTCCTACCC AGGAGTCCTGAATGTGATCTGAGTATGCTCTAAGGCAGCCCCAGGAAAAGCAATTCAGTCCCTCTCTCTT TGCCTTTAGACCTGCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTTTACAACCGTG AAGCCGGAAGATGCATATGAAGACTTTATTGTGAAACCTCCCGTGAGAAAGGTAGTCAAAGACAATTGCT TTAAGGGTTTAGGGCCGGGCGCGGTGGCTGATGCCTGTAATCCCAGCACTTTGGGAGGCCAAAGCGGGCA GATCACATGAGGTCAGGAGTTTGAGACCAGCCTGGCGAACATGGCAAAACCCCATCTCCACTAAAATACA AAAATTAGCTGGGTATGGTGGCAAGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCATGAGAATCAC TTGAACCTGGGAGGCAGAGATTGCAGTGAACCCAGATTGCGCCACTGCACTCCAACCTGGCAACAGAGTG AGACTCCATATCAAAAAAAGAAAAGAAGAAAAAGGGTTTAGGTGTTTTTTAATGCAAAAAGAAGCCTATA AAGATGGGGCGTGGTGGCTGATTCCTGTAATCCCAGCAGTTGGGAGGCTGAGGCAGGAGAATTGCTTGAA CCTGGGAGGCGGAGGTTGTAATGAGCCGAGATCGGGCCACTGTACTCCAGCCTGGGTGACAAGAGCGAGA CTCCATCTCGAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL834400 unedited GGTGCATATTAGGNATACGACTCATATAGGCGGCCGCGTATTCGGCACGAGGGTCTTTCT CTCACTGGNANAATCATAGTGTTGCTGGATGCATAAATATGAAGCTGTGGTCTCTGACAA GACTTGTGTTAGCAGATTGATGTTCAGGCTTCAGTATCCTCCTGACTTAACAAACAATTA CATTATTCATTCAGGAAACCTTATTCAGCACCCACTAGGTGCTCAGGTTCTCTGCAAAGG ACACAGAAATGAATGATACGTGTTCCTTGCATATGGCACTGTCTAGAGGGGACATACACA GCTGAAGAAGGGAAGGGCAGTGATGGGGTTATGGAGGAAGTATGGCAGGGAAAGCAGGAG GGTGATGCTGGAAGACAAATGGTAGATCCCAGGCGGACTGGAAAGAATTGCAATAGGGGG AGCCGGGGAGTGCAGTCTGTGAGAGGCTGGAAATGTGAAAGCCAGGAGCTCTCACGGATT TGGGTTGCCTCCTGCACAGTCAAGGAGAGTGTCACTCACGCTAGTCATAGATGGCATACC TACAGGCTTGGGCTGATTTTAGATAGTTGTTCAGAGCAGTCAAGCAAGCGCCAGATGCAC AAGCCCTGTGAGAGGTGCGTTTGGAATCCCTTGGTTTCCAATTACATGTCATCCCTTAGA GCAACGACACCCGTTGGATTTGGGGCTGGTCTTGAATGAAGATCCTTTTCTCATCTTCCC GAAGTTGCATGGCTTACGGGCAAATTTAAATCATGCCCTTGCCGTTTGCCCATTCGGTCC CTGGTGGTCCGTTACCTCCTTTGGTGACTCGGCAAAGCCACCAAGGGAATTTCGGGGTGG AACACCCTCGGCTCAACACCCTCTTGTTCACTATTACTTGTGGCCGATAATAACCATCGG TCAATTTAAAGGCCTTTTAACACCAATATTTATTTTCACTCCTTCCAAAGCTCCCCACCT CG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL834400 unedited ACCTGTTACGCGCCCGCTTTCTATAGTCGATTTTTTTTTTTTTTTTTAAAGTTCATCTCT CCTGCCCCAAAAAAACTGATAGCATGTGCCANTATCAGAAAAATCCTGGTCTTAGCAGAT CAATATACATAGTTATTTGGTCATTCGACATATGCACTTTTTAAGGAGGTTCAGGCTTCA TTTTCTGTTGAAGAGTTTGTTACCATATATTTTTTCTTTCGAGTCATTCTATCCAGTTCT TCCTGAACATTTGACAACACAGTCTTTTGTCCTGACTTCTTGGCTGTGCTCTGCACCCCA CCAGCACCAGGACTTCCAGGAGAGCCTGTCTTTTTACTCAACAGACTGTTGAAGAAGCTG GCCAACACCCCTTCACTTGCTGCATTATTTTTGATGTTTGGGTCCGGCTTTTTTACTGAC GTGCCTGGGGAGGAGCTAGGCACACTGGCTGGCCCTCCCCGACCCTGGGTCCTTGGAGAG CCAGAGGGTCCTCTTGCAGGAGATTCAGAAGCTCTCGTGNGAGTGGCTGGGTGCTTGGCA AGGAGTGACTGTTGCTTCATTAGGAACACCTGCTCATCTTCTGCTGCCAACTCTTTGTCG TGGACCAGCTTTCTCACGGGAGGTTTCACAATAAAGTCTTCATATGCATCTTCCGGCTTC ACGGTTGGAAAATTTTCATGTTAAATACCTATTTTCTTTTCATTGCCCAGCCCTGCGGTC TAAGGCAAGAAAAGAGGACTGAATGGTTTTCCTGGGCTGCCTTAAAGCTACTCGATCACA TTCGGACTCCTGGGTAGGACACTTCTCCAAAAAATATTGTGAAGTTTTTGGGGGTTGTTT GAACCTTTGGCAAAGTGCCAAAAACACCTGGGGATAAATCCTAAAGAAATCGAAGGCCCG GTCCATCCTGAATTAATGGCCCCAAGGTTAAG
Restriction Sites	NotI-NotI
ACCN	AL834400
Insert Size	2400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL834400.1
RefSeq Size	2197 bp
RefSeq ORF	2197 bp
Locus ID	54751
Gene Summary	This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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