TMEM126B (BC017574) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HT007; MGC111203; transmembrane protein 126B
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017574, the custom clone sequence may differ by one or more nucleotides ATGGCAGCATCTATGCATGGTCAGCCCAGTCCTTCTCTAGAAGATGCAAAACTCAGAAGACCAATGGTCA TAGAAATCATAGAAAAAAATTTTGACTATCTTAGAAAAGAAATGACACAAAATATATATCAAATGGCGAC ATTTGGAACAACAGCTGGTTTCTCTGGAATATTCTCAAACTTCCTGTTCAGACGCTGCTTCAAGGTTAAA CATGATGCTTTGAAGACATATGCATCATTGGCTACACTTCCATTTTTGTCTACTGTTGTTACTGACAAGC TTTTTGTAATTGATGCTTTGTATTCAGGTGAATTTAAATTCACTAATGTATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017574 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAAAAGGTGGTGTTCG GGTATGAGGCTGGGACTAAGCCAAGGGATTCAGGTGTGGTGCCGGTGGGAACTGAGGAAG CGCCCAAGGAAATGAAACACGATTTCCAAAATGAACTTAATCTTTCATGAGAAACTGAGG ATAGAGATGTCAATAAGCAGCCACTGTTTCCACCTCCCCACCTGAAGAGCTAGGAGGACA ACTACAAAGAGCCTGACTGCCTTCTCGGAATGAGGAGAGAGGAAAACAGCAACAGTATCA GTTTTCAAGATGGCAGCATCTATGCATGGTCAGCCCAGTCCTTCTCTAGAAGATGCAAAA CTCAGAAGACCAATGGTCATAGAAATCATAGAAAAAAATTTTGACTATCTTAGAAAAGAA ATGACACAAAATATATATCAAATGGCGACATTTGGAACAACAGCTGGTTTCTCTGGAATA TTCTCAAACTTCCTGTTCAGACGCTGCTTCAAGGTTAAACATGATGCTTTGAAGACATAT GCATCATTGGCTACACTTCCATTTTTGTCTACTGTTGTTACTGACAAGCTNTTTGTAATT GATGCTTTGTATTCAGATAATATAAGCAAGGAAAACTGTGTTTTCAGAAGCTCACTGATT GGCATAGTTTGTGGTGTTTTCTATCCCAGTTCTTTGGCTTTTACTAAAAATGGACGCCCC TGCACCAAGTATCATACCGTTCCACTGCCACCAAAAGGAAAGGTTTTAATCCATTGGATG ACCCTTTGTCAAACACAATGAAATAATGCCGATTCCTCTAGTCTTTCAGAATATGTTGGA TATTAAATGTCTATACATTATGCAGTATTTGAGAGACCTTGAGAAAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC017574 unedited NTCGGTTCAGCTATGNNACCGCGGCCCAANCTANGATCGAGTTTTTTTTTTTTTTTTTTT ACGTGTAAGATGAAACTTATATTTATTGATTGAATTATTGAATACTTTTTGAGTATTTGC TATATACCAGGCAAAAGGCACAGAACAAATTATTTGTTCACAGTTACTTTTAACTCTTTC AGCAATGCCTGAGTCCTCTTTATAGAAACTTCATTTTGCTAAGTTAGCAACCATTCATTT TTTTGGTTACTCTTCATGTATAGTTTTCTCAAGTGTCTCTTCAAATACTGCATAATGGTA TAGACCATTTAATATTCCAAACATAATCTGAAAGACTAGAGGAATCGCCATTAATTTCAT TTGTGTTTGACAAAGCGTCATCCAATGGATTAAAACCCTTCCTTTTGGTGGCAGTGGAAC GGTATGATACTTGGTTGCCAGGCGTCCATTTTTAGTAAAAGCCAAAGAACTGGGATAGAA AACACCACAAACTATGCCAATCAGTGAGCTTCTGAAAACACAGTTTTCCTTGCTTATATT ATCTGAATACAAAGCATCAATTACAAAAAGCTTGTCAGTAACAACAGTAGACAAAAATGG AAGTGTAGCCAATGATGCATATGTCTTCAAAGCATCATGTTTAACCTTGAAGCAGCGTCT GAACAGGAAGTTTGAGAATATTCCAGAGAAACCAGCTGTTGTTCCAAATGTCGCCATTTG AATATATTTTGTGGCATTTCTTTTCTAAGATAGTCAAAATTTTTTTCTATGATTTCTATG ACCATTGGTCTTCTGAGTTTTGCATCTTCTAAAGAAGACTGGGCTGACCATGCATAGATG CTGCCCTTCTGAAAACTGAAACTGGTGCTGGTTTCCCTCCTCCTCATTCCGAGAAGCAAT CACGCCTCTTTGTAG
Restriction Sites	NotI-NotI
ACCN	BC017574
Insert Size	1150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC017574.1, AAH17574.1
RefSeq Size	1419 bp
RefSeq ORF	1419 bp
Locus ID	55863
Protein Families	Transmembrane
Gene Summary	This gene encodes a mitochondrial transmembrane protein which is a component of the mitochondrial complex I assembly complex. The encoded protein serves as an assembly factor that is required for formation of the membrane arm of the complex. It interacts with NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 13. Naturally occurring mutations in this gene are associated with isolated complex I deficiency. A pseudogene of this gene has been defined on chromosome 9. [provided by RefSeq, Apr 2017]

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