ATP5L (BC015128) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP5JG; ATPase subunit G; ATP synthase, H+ transporting, mitochond; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase g chain, mitochondrial; F1F0-type ATP synthase subunit g; F1Fo-ATP synthase complex Fo membrane domain g subunit
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC015128, the custom clone sequence may differ by one or more nucleotides ATGGCCCAATTTGTCCGTAACCTTGTGGAGAAGACCCCGGCGCTGGTGAACGCTGCTGTGACTTACTCGA AGCCTCGATTGGCCACATTTTGGTACTACGCCAAGGTTGAGCTGGTTCCTCCCACCCCTGCTGAGATCCC TAGAGCTATTCAGAGCCTGAAAAAAATAGCCAATAGTGCTCAGACTGGTAGCTTCAAACAGCTCACAGTT AAGGAAGCTGTGCTGAATGGTTTGGTGGCCACTGAGGTGTTGATGTGGTTTTATGTCGGAGAGATTATAG GCAAGCGGGGCATCATTGGCTATGATGTTTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015128 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGTCCTTCCGGCGGGTG ACATTCAGCCGGCGGTTCGGGGCGACGGACTCTCCATTCCAGAACCATGGCCCAATTTGT CCGTAACCTTGTGGAGAAGACCCCGGCGCTGGTGAACGCTGCTGTGACTTACTCGAAGCC TCGATTGGCCACATTTTGGTACTACGCCAAGGTTGAGCTGGTTCCTCCCACCCCTGCTGA GATCCCTAGAGCTATTCAGAGCCTGAAAAAAATAGTCAATAGTGCTCAGACTGGTAGCTT CAAACAGCTCACAGTTAAGGAAGCTGTGCTGAATGGTTTGGTGGCCACTGAGGTGTTGAT GTGGTTTTATGTCGGAGAGATTATAGGCAAGCGGGGCATCATTGGCTATGATGTTTGAAG ACCAATCTTTAACATCTGATTATATTTGATTTATTATTTGAGTGTTGTTGGACCATGTGT GATCAGACTGCTATCTGAATAAAATAAGATTTGTCAAAACTCAGTGTTTTCTCCATCAGA TACTCCATGAAAGGTCACAATTTCTCTTGATATTAAGCTGGGTTGTCTTTAAACAACCCT AAATACACGTCTGTTTAGCCCGCAATTGGAAAGGATATATGTGGCAATATTAACCTGGTA CATGAATATATGGNGATAACATTTTAATTTGAAGGTTTGGAATATATATATTTAAGCTTT ATTTCCAGAACAGTGAGGGTTAGGTCTTGGGAAAACTATACTGCCANAGTAGAAGAATAG TAGTACATATGCANAGTGATAGAATGAATCTGTCAGTAGTTGAATACATTTCACTGGTTT CTTGCTAAATACAGAAGACCTATGACACCTCTAGTCTGTAAATGTANAGACTGTCATCTG AATTACCTCCCAGAAGACAGTTGTATCTAAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC015128 unedited GTACATCTAGNNACCGCGGCCGCATGCTAGNGATCGATTATTTTTTTTTTTTTTTTTTTT TTTATAGCAATCATTTCATTTATTTCAGGGAATAATATACTATTTAGATCCAGGGAAGAT GCTAAATTATAACCTTGTTTAAATATCTTTGGGGTATTTTGTTTTTGCAAAGGTAGTAAA ACCAGACAAACGAATAACACACATAAAAATCAGATTTCACATGTATGGCTCTGTCCTACT GCTAAAGATGTTACAAAGAAACATACTTAGAATACCACCCTGTTCTCTCTTGGGGAGGCT ATATTCAAGATGACAAGTACTCTAACATTTTTACAGACATAGATGTTGTCAATAGGGTCT TTCTGTGATTTTAGCAAAGAAAACAGTTGAAATGTTATTCTAACTACTGACATGATTCAT CTCTATCACTTTGGCATATGGTACTACTATTTCTTCTACTTTGGCAAGTTATAGTTTTCC CAAGACCTAACCCTCACTGTTCTGGAAATAAAGCTTAAATATATATATTCCAAACCTTCA AATTAAAATGTTATCCCCATATATTCATGTACCAGGTTAATATTGCCACATATATCCTTT CCAATTGCGGGCTAAACAGACGTGTATTTAGGGTTGTTTAAAGACNACCCAGCTTAATAT CAAGAGAAATTGTGACCTTTCATGGAGTATCTGATGGAGAAAACACTGAGTTTTGACAAA TCTTATTTTATTCAGATAGCAGTCTGATCACACATGGTCCAACAACACTCAAATAATAAA TCAAATATAATCAGATGTTAAAGATGGGTCTTCAAACATCATAGCCCAAGATGCCCCGCT TGCCTATAATCTCTCCCGACTAAAACCACCTCAACACCTCAGTGGCCACCAACCATTCAG CACAGGTTTCTTTAATGGGGAGCGGTTT
Restriction Sites	NotI-NotI
ACCN	BC015128
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015128.1, AAH15128.1
RefSeq Size	1154 bp
RefSeq ORF	312 bp
Locus ID	10632
Domains	ATP-synt_G
Protein Pathways	Metabolic pathways, Oxidative phosphorylation
Gene Summary	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

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