NUP50 (AF267865) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPAP60; NPAP60L
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF267865, the custom clone sequence may differ by one or more nucleotides ATGTGGACTGAAATTTTCATCTTTTGTTTGAGAATCTATGAAGTGTATCATATACGTGGCCTAAAGCAAG GTGTGTATTTTGTTATTCTGAAATTGTTTTGCATCTGGACAAATACTAAATATCCCAGTGGCCTTTTTTT TTTTTTTTTAAACCTGTGTATCCATCTCATCCTTTTGCGCATTCCTAGTAAGCAAAAAAATTTGTTATGC CATCTTCATTATTCGAATTACAGACTGAAAAAATATGGCCAGTTTTTAAAGAAGTTTAGATTATGTTTTC CATGGAAGGACAAGTCTGACTGTTCATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF267865 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTTCCTTTTTT CCTAACATTCCCAGCAAATTTTTGCTGCTAAGACTATCACTGTTAAAGTGAAAATTACAG GGAAAAATGTGATGAATATACCGTAACTCAAAATGTGATATTTTCTTAAAATCACTCTTT TATGCTTTAGGAACTGGTTGGTCTCCACTTTGATTATTAGTGTAAAGAGCCTGAGTATAC GTGGATTTCATTGTAAAATTTAACTCCTTGTCTTTTACTTGGGGCACGGGGCCCCTGGAG GGCTTCCCTACTTTCCCCACTATGTTAACAGGTAATTCTGATTTATGCCTTTAGTTTGAC TTATTTTTAACAAAATATTAGAAGTTATGCTTTAAAATGTTTAATGTGGACTGAAATTTT CATCTTTTGTTTGAGAATCTATGAAGTGTATCATATACGTGGCCTAAAGCAAGGTGTGTA TTTTGTTATTCTGAAATTGTTTTGCATCTGGACAAATACTAAATATCCCAGTGGCCTTTT TTTTTTTTTTTTAAACCTGTGTATCCATCTCATCCTTTTGCGCATTCCTAGTAAGCAAAA AAATTTGTTATGCCATCTTCATTATTCGAATTACAGACTGAAAAAATATGGCCAGTTTTT AAAGAAGTTTAGATTATGTTTTCCATGGAAGGACAAGTCTGACTGTTCATAGGCTGATTT TCTTTAAGAGGATTATTCTGTTNTACAATTTCAATTCTAGATCACATTTTATATATNGCT GCATGCCAAAAAAAAAAAAAAAACTCACTCTAGATTGCGGCCCGCGTCATAGCTTGTTTC TGAAAGATNCCNNGGTGCATCCNTGTGACCCCTCCCATGCCTCTNCTGGCCTGNAAAGTG CACTNCATGCCCACCANCTTGTCTATAAAATAAGTGCATCATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF267865 unedited AGCTATGAACCGCGCGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCATGCA GCATATATAAAATGTGATCTAGAATTGAAATTGTAAAACAGAATAATCCTCTTAAAGAAA ATCAGCCTATGAACAGTCAGACTTGTCCTTCCATGGAAAACATAATCTAAACTTCTTTAA AAACTGGCCATATTTTTTCAGTCTGTAATTCGAATAATGAAGATGGCATAACAAATTTTT TTGCTTACTAGGAATGCGCAAAAGGATGAGATGGATACACAGGTTTAAAAAAAAAAAAAA AAGGCCACTGGGATATTTAGTATTTGTCCAGATGCAAAACAATTTCAGAATAACAAAATA CACACCTTGCTTTAGGCCACGTATATGATACACTTCATAGATTCTCAAACAAAAGATGAA AATTTCAGTCCACATTAAACATTTTAAAGCATAACTTCTAATATTTTGTTAAAAATAAGT CAAACTAAAGGCATAAATCAGAATTACCTGTTAACATAGTGGGGAAAGTAGGGAAGCCCT CCAGGGGCCCCGTGCCCCAAGTAAAAGACAAGGAGTTAAATTTTACAATGAAATCCACGT ATACTCAGGCTCTTTACACTAATAATCAAAGTGGAGACCAACCAGTTCCTAAAGCATAAA AGAGTGATTTTAAGAAAATATCACATTTTGAGTTACGGTATATTCATCACATTTTTCCCT GTAATTTTCACTTTAACAGTGATAGTCTTAGCAGCAAAAATTTGCTGGGAATGTTAGGAA AAAAGGAAGCCACCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAA TTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTTACG NCCATTTGCGTCAACGGGGCGGGGGTTATACGACATTTTGAAAGTCCCGTTGATTTC
Restriction Sites	NotI-NotI
ACCN	AF267865
Insert Size	800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF267865.1, AAG44734.1
RefSeq Size	1485 bp
RefSeq ORF	1485 bp
Locus ID	10762
Protein Families	Stem cell - Pluripotency
Gene Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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