GNS (BC017742) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	G6S
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017742, the custom clone sequence may differ by one or more nucleotides AGAGCAGTAAGCTTGTGATAAAGGCCAATTCCAGGTAGCTCTTGAAGGTGATAGCCATCTACTTTCCAGT GGCTGCCAACCACAGGGAGTGCCAGTTAACACTGGAAGGATTAAGGCAAGGTCCCTTCTCTTGAGACTCC CCTCTGAGATCTGAAAAATGAAGTGGCTTAGGAACATCAGCAGTGAAGAACTGCCAAGAGTTGGTGAAGG TTGTCTCTTCCGAGGGCCTTCTGAAGACAGGGCTCTTGAACAGACAAGTGGAAGGGCTGTACCAGGGATA AAGGAAAGAAGTGCCTGTCCAGCAGGGAGCTTGAATTTAAGTTCCATGTATGAAGTCATTGGCTCTATCT GCATTTTTCTGTCATTCTCTTCATTTGTTTTAAGGTGGAAAATTTTCTTACAGTTGATGCAAAGTATCAA CTACTTTACCCTACCTTCTCCCCTTTTAGATGGGTTCTTCCTGAGTTTTGGAGTCTTGTATGATTATCAG TATTCCCCTGTCAAAATCAAATCTATTCAGGTTTCTTCACTGTTGAGAACACCTAAATGTTTTTATTTTT GAGAAGTGGGGACAGAGTCTCACTATGTCACCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCA ACCTTCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGGATTATAGGCACGCA CCACCACGCCCAGCTAATTTTTTGTATTTTTAGTAGAGACAGAGTTTCACCATGTTGGCCAGGCTGGTCT TGAACTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAGAGTGCTGGGATTACAGGCATGAGCCACCA CGCTTGGCTAAGAACACCTAAATTTTTATGTTTCTTGGCTCAAAAACCAGTTCCATTTCTAATGTTGTCC TCACAAGAAGGCTAATTGGTGGTGAGACAGCAGGGGAGGAGGAAGAGCTGTGGTTTGTAACTTGTTCAAC TCAGGCAATAAGCGATTTTAGCTTTATTTAAAGTCTTCTGTCCAGCTTTAAGCACTTTGTAAGACATGGC TGAAAGTAGCTTTTCTATCAGAATTGCAGATAGTCATGTTGGGCTAACAGTCAATTGGATATATTCCTTT ACCTCACATGACCCCAGCAACTGTGGTGGTATCTAGAGGTGAAACAGGCAAGTGAAATGGACACCTCTGC TGTGAATGTTTTAGAGAAGGAAATTCAAAAAATGTTGTAACTGAAAGCACTGTTGAATATGGGTATCGGC TTTCTTTTTCACTTTGACTCTTAACATTATCAGTCAACTTCCACATTAATGAAAGTTGACCATAGTTATT TCCAAATAAAAAGAAACCAACTCTTACCAGGTCTTGGACTGTGATGTCATATTATTCAGTTTTATGCTTG TTCCTGAGCAGAACTCATAAGAGTGACATAGTCAGCTGCTGACGGCACCTCAGCCACGCCACTCTTACTC AGTTCAGTGGGTGTGCTTGCGTGGTAGGATGTGGTGCAGCCCTCTCTACGCTCTTCTATTTTTGGTATAT TTCCTATCTAACCTTCAAATAGCTTCCAATTCTTTTTTTCTTGGACTGGCTTCATTCTGAATTTGTGCTA AAATAATCTTTCATAAAGAGACCTCAGTTTATAGCGTAACAGACTACACAATGCACTGATGTTTTCATAA TGTTTAAGGGACCCACTGCAAGAAGCTTGCTGCCTCCTTTTAATTGTATTCATTTAGATTTTGATTTTCC ATGTTAAGAAGGTGAGGTCCATGTTGGTGCCCTTCAGAGTAGAGAACCATGTAAACATTAGGAATGAACA GAGGCCTTAGGAATGAATAGAGAGTTTGCCTTATACAATTTCCTGTTACAAAGCTCTCCCTCTCATGCAA AGTAGGGAACACCTTTTGAGCATCTTTGAATTTGACAAATGGTGCTGTTGCAAACACTTTTTTTTTGAGA TGAAGTCTCGCGGTTGTCACCCGGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACTTCCACCTC CTGGGTTCCAGCAGTTCTCCTGCCTCAGCCTCCCAAGTAGCTGAGATTACAGGCGCCTGCCACCCCACCT GGCTGATTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGATTAACTCCTGACCTC AGGTGATCCACCTTTCTCGGCCTCCCAAAGTGCTGGGATTACGGGTGTGAGCCACCGTGCCCGGCCTGCA AACACATTTTAATTGACAACACTAGGGCTGTTGTACAAAATAGTAATGATAGCCATGGAAGTTTTACCTT ATTCTGTGAGAAGTGTTCTTAAACTTATTAAGTGTCTAAACTAAGGTTTAGTGCTTTTTTAAAGGAAAGT TGTCCCAGGATTCATCCTAAAGAAAGCAAAAGTTAATTCAACTGATCCACCAATGGAATTAGATGGGTAG AGTTGGGTTCTTGAGTTTTACCACCACTTAGTTCCCACTGAATTTTGTAACTTCCTGTGTTTGCATCCTC TGTTCCTATTCTGCCCTTGCTCTGTGTCATCTCAGTCATTTGACTTAGAAAGTGCCCTTCAAAAGGACCC TGTTCACTGCTGCACTTTTCAATGAATTAAAATTTATTTCTGTTCTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017742 unedited GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGGCTGGT GCTGCACTGAAATAGAGCAGTAAGCTTGTGATAAAGGCCAATTCCAGGTAGCTCTTGAAG GTGATAGCCATCTACTTTCCAGTGGCTGCCAACCACAGGGAGTGCCAGTTAACACTGGAA GGATTAAGGCAAGGTCCCTTCTCTTGAGACTCCCCTCTGAGATCTGAAAAATGAAGTGGC TTAGGAACATCAGCAGTGAAGAACTGCCAAGAGTTGGTGAAGGTTGTCTCTTCCGAGGGC CTTCTGAAGACAGGGCTCTTGAACAGACAAGTGGAAGGGCTGTACCAGGGATAAAGGAAA GAAGTGCCTGTCCAGCAGGGAGCTTGAATTTAAGTTCCATGTATGAAGTCATTGGCTCTA TCTGCATTTTTCTGTCATTCTCTTCATTTGTTTTAAGGTGGAAAATTTTCTTACAGTTGA TGCAAAGTATCAACTACTTTACCCTACCTTCTCCCCTTTTAGATGGGTTCTTCCTGAGTT TTGGAGTCTTGTATGATTATCAGTATTCCCCTGTCAAAATCAAATCTATTCAGGTTTCTT CACTGTTGAGAACACCTAAATGTTTTTATTTTTGAGAAGTGGGGACAGAGTCTCACTATG TCACCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCAACCTTCGCCTCCTGGGT TCAAGCGATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGGATTATAGGCACACACCACCAC GCCCAGCTAATTTTTTGTATTTTTAGTAGAGACAGAGNTTCACCATNGTGGCCAGNCTGG TCTTGAACTCCTGACN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC017742 unedited ATTTACTTGNACCGCGGCCCGCTATCTAGGATCGAGTTTTTTCTTTTTTTTTTTAGACAG AAATATTTTATTCTTGAAAATGCAGCAGTGAACAGGGTCCTTTTGAAGGGCACTTTCTAA GTCAAATGACTGAGATGACACAGAGCAAGGGCAGAATAGGAACAGAGGATGCAAACACAG GAAGTTACAAAATTCAGTGGGAACTAAGTGGTGGTAAAACTCAAGAACCCAACTCTACCC ATCTAATTCCATTGGTGGATCAGTTGAATTAACTTTTGCTTTCTTTAGGATGAATCCTGG GACAACTTTCCTTTAAAAAAGCACTAAACCTTAGTTTAGACACTTAATAAGTTTAAGAAC ACTTCTCACAGAATAAGGTAAAACTTCCATGGCTATCATTACTATTTTGTACAACAGCCC TAGTGTTGTCAATTAAAATGTGTTTGCAGGCCGGGCACGGTGGCTCACACCCGTAATCCC AGCACTTTGGGAGGCCGAGAAAGGTGGATCACCTGAGGTCAGGAGTTAATCAGCCTGGCC AACATGGTGAAACCCCGTCTCTACTAAAATTACAAAAATCAGCCAGGTGGGGTGGCAGGC GCCTGTAATCTCAGCTACTTGGGAGGCTGAGGCAGGAGAACTGCTGGAACCCAGGAGGTG GAAGTTGCAGTGAGCCGAGATCACGCCACTGCACTCCAGCCCGGGTGACAACCGCGAGAC TTCATCTCAAAAAAAAAGTGTTTGCAACAGCACCATTTGTCAAATTCAAAGATGCTCAAA AGGTGTTCCCTACTTTGCATGAGAGGGAGAGCTTTGTAACAGGGAATTGTATAAGGCAAA CTCTCTATTCATTCCTAAGGCCTCTNGTCATTCCTATGTTTACATGGNTCTCTACTCTGA AGGGCACCACATGGACCTCACCTTCTAC
Restriction Sites	NotI-NotI
ACCN	BC017742
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017742.1
RefSeq Size	2651 bp
RefSeq ORF	2651 bp
Locus ID	2799
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Glycosaminoglycan degradation, Lysosome, Metabolic pathways
Gene Summary	The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq, Jul 2008]

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