TMEM181 (BC021687) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC021687, the custom clone sequence may differ by one or more nucleotides GGGGAAAAAATAACTGACTTGCTTAACCAGAAAACAGAATGTGGGTCCCAGTACAGCATATAATGAAACC AGTTAGAGACAACAACAGGCAAGAAAAGATGATTTGGCAAGGTTGGAGGTCCTAATGTGAGCAATCAACC TCTAAATAATTCTAAAATCACTGACATGATCCAGATGATACCCAAAGATGGTGGCATTGGGTACTGACTC TAGGACATACTAAAGCTGTTTTCATGGGTTATAAGGTAAGATGAAAAACTGAGGGAAAGAAACAAGTCGT TGAAGTAATTTCAGCTATAGGATGAGAATGTTCTAGATCAGGATTGTTGAGTAAGTAAGGGATAAATTAA AAGGGAAAGAAGTAGATGCCAGGCAAGGGAAAGAGTCAAGAAATTCAGAAATGTGGGAGCTAGTGAAGTC TGGAAGGCAGTGGTCTTGCTCAAGTACTACTGGGGTGTCAGACTGTGCCCCACTTACAGGTAGTACAGGT GTAGGACAGGAAAGAGTGGGTAGGAAATAACCAGGTGCAGTCGTTAACCTGGTATTGATCTACCCATGGG TGATAAATGAAATCAAATCAGATGAAAGTCCTCTATAATGAAAGAAAAGAGAGAGGCACAAACTGACCCA AGAGCTATGGGATCCTGTTCACCAGTACTGGGCCAGAAAAAAAAAAAAAGGTTTAAATACTGAGTGTGTA AGCCAGCTCCCCCCTTCATATGTATTCCTGCGATATATATATATACATATGGATATCCTGCAGAACAACA TGGCTATTTGCCCTCTAACATGCTCTAAAAACCCTTTCTCCAACCTGTGCATTTATTTAGATAGACCTAG AACGTGGAAACACCAGCACAATCTGGTTTCTCAGATGCCCATCGTATTGGTTCCTGTCCCTGCCTTACGG TACCCATCATGTTATCATTTATGTGCAAACAAGCTGTGGTGTACTGGAGGGAAGGGAAGGGATGCACACA GCAGGCCTTTTTTGTTTTTGTTTGTTTGTTTTGTGACAGAGGAAGGAGGTATGAGGTTGTACTTTATTTT TAAGAAAATGACAGTTCGATTCAGATTAGCTTTATATTTTTTGAACTGGCTTCCAGTTGGCCTCTAGGTG GGGGCCTCCACTCAACGGCTGCTATTGTAGACCCCTCCCCAATTCTGGCTCCACAAAAGGGGCAGGAATA AACAGTATAGGGCAGATGTTGGCTGAAAGGTGTAATTAAGTATACTTTTGACTTAAACATACTGTCTTAA AGAGATCCTATCTCTTTTACGGAATGAGTTGTTAGAAAATCTTCTGTATCTAACGTGAAAGCTCACAGGG TACAACTGCACTTAAATTGTCAGTTAGGACAAGCTTTAACCTCTCGTGACAAAACCCCTAAAGGAGGTAT CTGCCCTGAGGGATATGAAGCTACAAGTTGTAATGAAGGATTCTGTTTCATAGGACATGCTAACATGTAA TGCTCTGCATTTTAGTTATTAAAATGAACCTTTTTAAAGCTTAAGGATGACAAACTCAGGGTTTCCTTAT ACCACTGAGTTTTTAAAAGCCAGTGGTGAGTTTAGATGTTAGCAATTCAGTGGAACTATGAAAAAGATGG AATTGTGATGGCTTTCCATAACTAATTTCTGCTTCACAAATATAAAATAGCTATTTTGAAATTCCAAATT GACTAGACAGACTACGGCTTGTCCCTGAAGCCATTGTTGAAGACAAACTTACAACTCTTATTAGAAGTAG CAGCTTCATATTTAAAGAAGAAAGTCTTTTTGAATTTTGCCAATTAAATTACCAGTGACCTTTAAGAAGT AAGTCAAAGGTGTCTTAGAGGGAAAATTTGAAGTAACAGAGACAGGCAGCAGCATCAGAGATAAGAGCTG ATAGTCAAGCATTGAATGAAGGAAATCAAGACAGTGGTGAGATCTCACGGCAGGCAGAGGCTGAGGCTGA AAGCAAGGCAGTGGGCTGATGTTCAGGGTCCTATTAGACACTTTTCTGAGGTTAAGGATGGACTTTTTAA GAGGCTCCTAAGAGTTACATGTCCATGGCTTGAACTTTTTGTCTCAAACAACAGAAACATAGGAACTAGG AACAATAACCTAAAACTGGGGCTTTATGTGGTTCTGGGTAGCAGACAAATTGAGTCACTTGAATTTCCAG AGTACAAAAGACCCCTTTAACCCCTTCCTGAAAAAGATTTTTCATGGTCAGGTATTTTTAAATACTTGCA TACTTTGTTCAGAAATCTGTATACATTAAAAAAACATGTTCTCTTTAAGAATATTCTGGCCAGGCACGGT GACTCTTGTCTGTAATCCCAACACTTTTGGAGGCCAAGGCGGGAGGATCACTTGAGCCCAGGAGTTTGAG ACCAGCGTGGGTATAGTGAGACCTTGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021687 unedited ACGNAGGGAATNNAAATTAAAAGGGNAAAGNAAGTAGNATNNGCCAGGCAAGGGGAAAGA GTCAAGAAATTCAGAAATGTGGGAGCTAGTGAAGTCTGGAAGGCAGTGGTCTTGCTCAAG TACTACTGGGGTGTCAGACTGTGCCCCACTTACAGGTAGTACAGGTGTAGGACAGGAAAG AGTGGGTAGGAAATAACCAGGTGCAGTCGTTAACCTGGTATTGATCTACCCATGGGTGAT AAATGAAATCAAATCAGATGAAAGTCCTCTATAATGAAAGAAAAGAGAGAGGCACAAACT GACCCAAGAGCTATGGGATCCTGTTCACCAGTACTGGGCCAGAAAAAAAAAAAAAGGTTT AAATACTGAGTGTGTAAGCCAGCTCCCCCCTTCATATGTATTCCTGCGATATATATATAT ACATATGGATATCCTGCAGAACAACATGGCTATTTGCCCTCTAACATGCTCTAAAAACCC TTTCTCCAACCTGTGCATTTATTTAGATAGACCTAGAACGTGGAAACACCAGCACAATCT GGTTTCTCAGATGCCCATCGTATTGGTTCCTGTCCCTGCCTTACNGTACCCATCATGTTA TCATTTATGTGCAAACAAGCTGTGGTGTACTGGAGGGAAAGGAAGGGATGCACACAGCAG GCCCTTTTTTGTTTTTGTTTGGTTGTTTTGGGACAGAAGAAAGAGGTATGANGTTGTACC TTTATTTTAAAGAAATGACAGTTCGATTCAGATTAGCTTTATATTTNTTGAACTGGCTTC CAGTTGCCTCTAGGTGGGGGCTCACTCAACGCTGCTATTGTAGACCCCTCCCAATTCTGG CTCCCAAAAGGGGCAGNGATAACATATAGGGCAGATGTTGCTGAAAGGGTAATAANTTAC TTTTGACTTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC021687 unedited NNCCGGTTCTACTATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTT TTTTTTTTTTGAGACAAGGTCTCACTATACCCACGCTGGTCTCAAACTCCTGGGCTCAAG TGATCCTCCCGCCTTGGCCTCCAAAAGTGTTGGGATTACAGACAAGAGTCACCGTGCCTG GCCAGAATATTCTTAAAGAGAACATGTTTTTTTAATGTATACAGATTTCTGAACAAAGTA TGCAAGTATTTAAAAATACCTGACCATGAAAAATCTTTTTCAGGAAGGGGTTAAAGGGGT CTTTTGTACTCTGGAAATTCAAGTGACTCAATTTGTCTGCTACCCAGAACCACATAAAGC CCCAGTTTTAGGTTATTGGTCCTAGTTCCTATGTTTCTGTTGTTTGAGACAAAAAGTTCA AGCCATGGACATGTAACTCTTAGGAGCCTCTTAAAAAGTCCATCCTTAACCTCAGAAAAG TGTCTAATAGGACCCTGAACATCAGCCCACTGCCTTGCTTTCAGCCTCAGCCTCTGCCTG CCGTGAGATCTCACCACTGTCTTGATTTCCTTCATTCAATGCTTGACTATCAGCTCTTAT CTCTGATGCTGCTGCCTGTCTCTGTTACTTCAAATTTTCCCTCTAAGACACCTTTGACTT ACTTCTTAAAGGTCACTGGTAATTTAAATTGGAAAAATCAAAAAGACTTTCTTTCTTTAA TATGAAGCTGCTACTTCTAATAAGAGGTGTAAGTTTGGCTTCCACAATGGCTTTCGGGAC CAACCCGTAATCTGGCTAGTCAAATTGGGATTTCAAAATAGCTTTTTTATATTTGTGAAG CACAAATTTATTTTTGGAAAGCCTTCACCAATTCATCTTTTTTATAGTTCCCCTGAATT
Restriction Sites	NotI-NotI
ACCN	BC021687
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021687.1
RefSeq Size	2439 bp
RefSeq ORF	2439 bp
Locus ID	57583
Protein Families	Transmembrane
Gene Summary	The TMEM181 gene encodes a putative G protein-coupled receptor expressed on the cell surface (Carette et al., 2009 [PubMed 19965467]; Wollscheid et al., 2009 [PubMed 19349973]).[supplied by OMIM, Jan 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.