LRRC8D (BC009486) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ10470; FLJ20403; leucine-rich repeat-containing 5; leucine rich repeat containing 5; leucine rich repeat containing 8 family, member D; LRRC5; OTTHUMP00000012307
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC009486, the custom clone sequence may differ by one or more nucleotides ATGGTCAGTAGCAACTTTTGGTTCAAATATCCCAAAACATGCTCAAAAGTAGAACATTTTGTTTCAATAT TAGGAAAGTGCTTTGAATCCCCTTGGACGACAAAAGCGTTGTCTGAGACAGCATGCGAAGACTCAGAGGA AAACAAGCAGAGAATAACAGGTGCCCAGACTCTACCAAAGCATGTTTCTACCAGCAGTGATGAAGGGAGC CCCAGTGCCAGTACACCAATGATCAATAAAACTGGCTTTAAATTTTCAGCTGAGAAGCCTGTGATTGAAG TTCCCAGCATGACAATCCTGGATAAAAAGGATGGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAA GTTCCGTGCCCATGTGGAAGATAGTGACTTGATCTATAAACTCTATGTGGTCCAAACAGCTTCTCCCTTT CCCAACCAATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009486 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGTCCCGGTGCAGCCGC CGCCCGCCGCGGGTGATGCCGCCACCTCCGGCCTCAGCATAAGCCGTGGCTTGGCGGCCG AGCTGCACCCCAAGGAAGTGCACGGCTGTCTATAACGTGCTGCCGGGTCTCAGGATGGAG GAGTGAAGTCTCCTGTCGCCGTGGTTCCAGCCTCCGGAGCTCGCCCAAGCCGCGTCCCCA GAGAGCGCCCTGAGAGAACAGGGTGGCCGCTTGGTCCAGGAATGTTTACCCTTGCGGAAG TTGCATCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAACCATGGTGGGATGTGT TTATGGATTACCTAGCTGTTGTTATGTTAATGGTAGCCATCTTTGCAGGAACCATGCAAC TTACCAAAGATCAGGTGGTCTGTTTGCCAGTATTGCCATCTCCTGTAAATTCAAAGGCAC ATACACCACCAGGAAATGCCGAGGTCACCACCAACATCCCAAAGATGGAAGCAGCCACCA ACCAAGACCAAGATGGGCGGACAACAAACGACATTTCCTTTGGGACATCTGCTGTGACAC CTGACATACCTCTCAGAGCCACATATCCTCGCACAGATTTCGCACTTCCAAATCAGGAGG CAAAGAAAGAGAAGAAAGATCCAACAGGTCGAANAACAACTTGGATTTTTCAGCATATGT ATTTATTAATCAAATGTGTTACCATCTGGCCCTTNNCGTGGTATTCTAAGTACTTTCCAT ACCTAGCTCTTATACATACTATTATTCTCATGGGCAGTAGCACCTTTTGGNTCAATATCC CANACATGCTCAAAGTAGACATTTTGTTCATATTANGAAAGGCTTGAATCCCTTGACACC AAACGTGTCTGAGAACATGCAA
Restriction Sites	NotI-NotI
ACCN	BC009486
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC009486.1, AAH09486.1
RefSeq Size	1361 bp
RefSeq ORF	432 bp
Locus ID	55144
Protein Families	Transmembrane
Gene Summary	Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658, PubMed:28193731). Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24782309, PubMed:24790029, PubMed:26824658, PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Mediates the import of the antibiotic blasticidin-S into the cell (PubMed:24782309).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.