SFRS3 (SRSF3) (AF107405) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SFRS3; SRp20
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF107405, the custom clone sequence may differ by one or more nucleotides ATGCATCGTGATTCCTGTCCATTGGACTGTAAGGTTTATGTAGGCAATCTTGGAAACAATGGCAACAAGA CGGAATTGGAACGGGCTTTTGGCTACTATGGACCACTCCGAAGTGTGTGGGTTGCTAGAAACCCACCCGG CTTTGCTTTTGTTGAATTTGAAGATCCCCGAGATGCAGCTGATGCAGTCCGAGAGCTAGATGGAAGAACA CTATGTGGCTGCCGTGTAAGAGTGGAACTGTCGAATGGTGAAAAAAGAAGTAGAAATCGTGGCCCACCTC CCTCTTGGGGTCGTCGCCCTCGAGATGATTATCGTAGGAGGAGTCCTCCACCTCGTCGCAGATCTCCAAG AAGGAGAAGCTTCTCTCGCAGCCGGAGCAGGTCCCTTTCTAGAGATAGGAGAAGAGAGAGATCGCTGTCT CGGGAGAGAAATCACAAGCCGTCCCGATCCTTCTCTAGGTCTCGTAGTCGATCTAGGTCAAATGAAAGGA AATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF107405 unedited TTCAAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGTGAGT GAGAGAGTTGGTTGGTGTTGGGCCGGAGGAAAGCGGGAAGACTCATCGGAGCGTGTGGAT TTGAGCCGCCGCATTTTTTAACCCTAGATCTCGAAATGCATCGTGATTCCTGTCCATTGG ACTGTAAGGTTTATGTAGGCAATCTTGGAAACAATGGCAACAAGACGGAATTGGAACGGG CTTTTGGCTACTATGGACCACTCCGAAGTGTGTGGGTTGCTAGAAACCCACCCGGCTTTG CTTTTGTTGAATTTGAAGATCCCCGAGATGCAGCTGATGCAGTCCGAGAGCTAGATGGAA GAACACTATGTGGCTGCCGTGTAAGAGTGGAACTGTCGAATGGTGAAAAAAGAAGTAGAA ATCGTGGCCCACCTCCCTCTTGGGGTCGTCGCCCTCGAGATGATTATCGTAGGAGGAGTC CTCCACCTCGTCGCAGATCTCCAAGAAGGAGAAGCTTCTCTCGCAGCCGGAGCAGGTCCC TTTCTAGAGATAGGAGAAGAGAGAGATCGCTGTCTCGGGAGAGAAATCACAAGCCGTCCC GATCCTTCTCTAGGTCTCGTAGTCGATCTAGGTCAAATGAAAGGAAATAGAAGACAGTTT GCAAGAGAAGTGGTGTACAGGAAATTACTTCATTTGACAGGAGTATGTACAGAAAATTCA AGTTTTGTTTGAGACTTCATAAGCTTGGTGCATTTTTAAGATGTTTTAGCTGTTCAAATC TGTTTGTCTCTTGAACAGTGACACAAAGTGTATTCTCTATGGTNTGAAATGGACATACGA GGCATGTATACCAAGAATGNTACTTTACATGTTCCCTTAGCAAAATGATTTGCTTGAACT TTAGTATGCCAGACTGATATAACTCTAACTGCCACGNAGGGTGNTTTTTAAATAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF107405 unedited GCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAACAATTTGTAACCAAAACTTT AATCCCAAGGATTCTCACAAAACATATTACAAATGACAGCATGAAAAAAAAATCTTGCAC AGTAACTCAAAGTTCGGCTCTACAATGTACCCTTAAACTGGCAGGACATTTTTGAAATCA CAAATTTGCACATAAAGAATGTCACGAACAGCCATGTATCCATATACAGCAATCAAATAA GGAACTTATGACCTAAAGCAAAGGTAAACTTTCTTGAAACTTAACATTCTATACCAACTA GGCAACCTCTGCCCAGGATGAGAGTTGGATTTTTCAAAAACCTCTAATTTAATAGTGCAG CATTTCGTTTTCCCTGATGGCCTGTGTTTCACAGCAGTTTTTAAAGACTGCTTGTTCAAC TATAGCTGCAGCCTATATCCCAGCTATGGAAAAAAAAGTAAATCTTAGTTCAATTTTTGC CAGTTGTTTCTGTATTTAAATTTAAAAAAAAACACACTTCCGCTGGGCAGGTTTAGAGGT TTATTATCAGTCTGTGCATAACTAAAAGTTCAAAGCAAATTCAATTTTGCTTAAGGGAAC ATTGTAAAGTAACAATTCTTGGTATTACATGCCTCGTATGATCCATTTCAAACCATAGAG AATTACACCTTTGTGTCACTGTTTCATGAGACAAACAGATTTGAACAGCTAAACATCTTA AAAATGCACCAAGCTTATGAAGTCTCAAACAAAACTTGAATTCTCTGTACATACTCCCTG TCAAATGAAGTTATTTTCCTGTACACCACCTCCTCTTGCAAACCTGTCTCCTATTCCCCT CCATCTGACCTAGATCGACTACCAGACCTANAGAAAGNATCCGGACGGCTTGTGATTTCT CTTCCGAAAAGCGATCCCTCTCTTTCCTATCCTATAAAGGACCGCTCCGCTGGAAAGAAC TTCTCTCTGGGGATCGGACAGTGAAGACCCTCTACAATN
Restriction Sites	NotI-NotI
ACCN	AF107405
Insert Size	1430 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF107405.1, AAD44523.1
RefSeq Size	2060 bp
RefSeq ORF	495 bp
Locus ID	6428
Domains	RRM
Protein Families	Stem cell - Pluripotency
Protein Pathways	Spliceosome
Gene Summary	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]

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