PUS7 (AK091283) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091283, the custom clone sequence may differ by one or more nucleotides ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTG GAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGTCTAACCAAAGGTCAAGATGGGCT ACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAAGGT GGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAGTGCG AGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGCATCAC CAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCAT GAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTT CAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTGTTCAAAAA TAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATCCATCAGGCT AACAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATACATTGTAGCCT ACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCTAGGGGAAGTTA CTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGCACTCTCCAAATACTTA AGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACAGTTCAAGAAATTG CTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAACTTTAAGCTAGGGAA TTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTCACTGTTGTTCTCAGA AATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAGATTGGATTTATTAACT ACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGAAGAGCTATACTACAAAA TTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAAAAGGGCTACTTGGTTAAA TGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCTGTCAAAAGGTGTG TGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAAT ACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTGTGGAATAACATGGTAAGCAAG AGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATA TTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTAT CTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGACAAC ATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATTCGTCCTCAGAATG TTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTCAACACAGATGTGGACAACCTAGA AGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAAATGGATTTTTCTCTACCC CCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAAATGGATACCAGTATCAAGAACCAGACGC AGCTGAATACAACCTGGCTTCGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091283 unedited GGGGGGGGGNNNNNTTTCCTCCNNNNNNGGTTTANAATTGTAAAACCACTTTCTATAGGC GGCCGCGNAATTCGCACGAGTCGGCTGNGACGCATCTGGTCCTCCGCGCGGAAAGCGCTG CTTTTGCCTGGCCGCCCTAGCCGCTGGCTCATCCAAGTGGCCTTCGCCGCTCTCTTGCGT CCCAACCAGAGCGCTGGCCACCTCGCCGCCCAGCTCACGCCGCGCCCGCGCTCCCAGGCT CCGGGTTTTCTTAAATGTTTTCTTGGAGCCTTAAAGATGGAGATGACAGAAATGACTGGT GTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTGGAGTCCCAGTTGAA GAGACAAAAAAACAGAAGCTGTCGGAATGCAGTCTAACCAAAGGTCAAGATGGGCTACAG AATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGG AAAGGTGGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGA CTTTCAGAGGAGTGCGAGGAGGAGGAATCANAGAGTTTTGCAGACATGATGAAGCATGGA CTCACTGAGGCTGACGTAGGCATCACCAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGA ATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCATGAAATAGGAAAAGATGGACGGATC AGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTTCAGAGACATATTTA CAGNTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTNCAGCTGTTCAAAAATAAGG AACCAGTGTTGCCATTGAGAGTCAAGCCAATATATTCTCCTACATGGGAACCAAGATAAA GGGNCTATACAGNTCAGNAANNATGCTGTCTCAAAATACTGCACAAGACTTGCCCACTGA ATAAGTGCTTGATGACCTTTAGCTAGGNAAATTCAGCTTCAAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091283 unedited NCGTCACTATNACCGCGGCCCAATCTATGATCGGTTTTTTTTTTTTTTTTTGAGATTTTA AAAATATTTATTACATTTGTTTCTAGAAATCATAGAAAATAAAAATTGATACAATTTTGA TATACAACTTTAGAAAGGCATATTTATCCTTTCACACCAGAGAAGTTTATGCAATGCCAG ACATGGAAATACCAAAGCCACTGGTGACAAAGGGTAAACGCTACTGATAGAAGAAAGCCT TGTCCATTTTTATAGTTTTCTTCTCTACCATTATTTATTTTTGAGCAAATACCAGTCCAG TGGAAAGAGTTCTGAACATAAAAGGCACCTAGAGTAAAAATTAGACCTTAACTAATAAAA CAGCTGTACACGTTTACATCAAGAAAAAAAAACCCGGCCAGCACAGCTCATGCCTGTAAT CCCAGGAGTTAGCCTCGGCAACATGGCCAAACCCCCGTCTCTACAAAAAAATACAAAAAT CAGCTAGGCGTGGTGGTGCGCGCCTGTACTCCCAGCTACTTATGAGGCTGANGTGGGAGA GTCACTGGAGCCTGGGAGGTCGAGGCTGCAGTGCAAGATTGANGATTGCANGATTGCACT GCAATCCAGCCTGTGCTACAGAGTGAGATCCTGTCTCGNAAAANAAAATAAAAAAATTAA ATGGGCCTATCAATGGCACGTTCCTGCAAAGCATTGATAATATAAATGCCAAAGCCACCG CAACCAAAAAGTCTATAATGGCTGGCCTGAATCTAGTACATAAGCAGGATAATGGACATC ACTTTTTGGTTATAAATTTGGAGATGGCCTATTATTTCAGTCAGCATGAGTACCGTATTT ATATTGGGGGTTGGCAACTCTAAATATTTATCCCTAC
Restriction Sites	NotI-NotI
ACCN	AK091283
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091283.1, BAG52324.1
RefSeq Size	2691 bp
RefSeq ORF	2691 bp
Locus ID	54517
Domains	UPF0024
Gene Summary	Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function]

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