MANSC1 (BC032998) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	9130403P13Rik; FLJ10298; LOH12CR3; MANSC domain containing 1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC105495 representing BC032998. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTCTTCGGGGGAGAAGGGAGCTTGACTTACACTTTGGTAATAATTTGCTTCCTGACACTAAGGCTG TCTGCTAGTCAGAATTGCCTCAAAAAGAGTCTAGAAGATGTTGTCATTGACATCCAGTCATCTCTTTCT AAGGGAATCAGAGGCAATGAGCCCGTATATACTTCAACTCAAGAAGACTGCATTAATTCTTGCTGTTCA ACAAAAAACATATCAGGGGACAAAGCATGTAACTTGATGATCTTCGACACTCGAAAAACAGCTAGACAA CCCAACTGCTACCTATTTTTCTGTCCCAACGAGGAAGCCTGTCCATTGAAACCAGCAAAAGGACTTATG AGTTACAGGATAATTACAGATTTTCCATCTTTGACCAGAAATTTGCCAAGCCAAGAGTTACCCCAGGAA GATTCTCTCTTACATGGCCAATTTTCACAAGCAGTCACTCCCCTAGCCCATCATCACACAGATTATTCA AAGCCCACCGATATCTCATGGAGAGACACACTTTCTCAGAAGTTTGGATCCTCAGATCACTTGGAGAAA CTATTTAAGATGGATGAAGCAAGTGCCCAGCTCCTTGCTTATAAGGAAAAAGGCCATTCTCAGAGTTCA CAATTTTCCTCTGATCAAGAAATAGCTCATCTGCTGCCTGAAAATGTGAGTGCGCTCCCAGCTACGGTG GCAGTTGCTTCTCCACATACCACCTCGGCTACTCCAAAGCCCGCCACCCTTCTACCCACCAATGCTTCA GTGACACCTTCTGGGACTTCCCAGCCACAGCTGGCCACCACAGCTCCACCTGTAACCACTGTCACTTCT CAGCCTCCCACGACCCTCATTTCTACAGTTTTTACACGGGCTGCGGCTACACTCCAAGCAATGGCTACA ACAGCAGTTCTGACTACCACCTTTCAGGCACCTACGGACTCGAAAGGCAGCTTAGAAACCATACCGTTT ACAGAAATCTCCAACCTAACTTTGAACACAGGGAATGTGTATAACCCTACTGCACTTTCTATGTCAAAT GTGGAGTCTTCCACTATGAATAAAACTGCTTCCTGGGAAGGTAGGGAGGCCAGTCCAGGCAGTTCCTCC CAGGGCAGTGTTCCAGAAAATCAGTACGGCCTTCCATTTGAAAAATGGCTTCTTATCGGGTCCCTGCTC TTTGGTGTCCTGTTCCTGGTGATAGGCCTCGTCCTCCTGGGTAGAATCCTCTCGGAATCACTCCGCAGG AAACGTTACTCAAGACTGGATTATTTGATCAATGGGATCTATGTGGACATCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032998 unedited NNNNGGGGGGGNNNNNNNANNCNNNNNNNCCGTTCAGATTTGTATACGACTCACTATAGG CGGCCGCGNAATTCGCACCAGCGGGNACTGCGGNACATCCGCTGCGGTCCTCGCCGAGAC CCCCGCGCGGATTCGCCGGTCCTTCCCGCGGGCGCGACAGAGCTGTCCTCGCACCTGGAT GGCAGCAGGGGCGCCGGGGTCCTCTCGACGCCAGAGAGAAATCTCATCATCTGTGCAGCC TTCTTAAAGCAAACTAAGACCAGAGGGAGGATTATCCTTGACCTTTGAAGACCAAAACTA AACTGAAATTTAAAATGTTCTTCGGGGGAGAAGGGAGCTTGACTTACACTTTGGTAATAA TTTGCTTCCTGACACTAAGGCTGTCTGCTAGTCAGAATTGCCTCAAAAAGAGTCTAGAAG ATGTTGTCATTGACATCCAGTCATCTCTTTCTAAGGGAATCAGAGGCAATGAGCCCGTAT ATACTTCAACTCAAGAAGACTGCATTAATTCTTGCTGTTCAACAAAAAACATATCAGGGG ACAAAGCATGTAACTTGATGATCTTCGACACTCGAAAAACAGCTAGACAACCCAACTGCT ACCTATTTTTCTGTCCCAACGAGGAAGCCTGTCCATTGAAACCAGCAAAAGGACTTATGA GTTACAGGATAATTACAGATTTTCCATCTTTGACCAGAAATTTGCCAAGCCAAGAGTTAC CCCAGGAAGATTCTCTCTTACATGGCCAATTTTCACAAGCAGTCACTCCCCTAGCCCATC ATCACACAGATTATTCANAGCCCACCGATATCTCATGGAGAGACACACTNTCTCAGAGNN TTGGATCCTCAGATCACTTGGNAGAACTATTTAGAAGGATGAAGGCAGTGCCCAGCTCCT TTGCTATANGGAAAAAGGNNCATNTCTCAGAGTCACAAATTTTCCTCTGATCAAGAAATA GCTCANCTGCTGCCTGAANAATGTGAGGGCGCTCCCAGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC032998 unedited ANCGAGATTTTCTTTTTTTTTTCTTTTATTCAAATATTTAATTGGGAAGGAACTACTCTG GGAATAAGTTTTAAAGGAATCCATATAATAAGGAAAAGCAAATCCATTAGGAAATTGATA TAAACAGTTGATTTTATCTGGAACCAAGAATGTGAATGAATTGGAACCTAGATGTCCTAA CCTGTTTCTTTGCATAAAAGCCAGTTGAATTTTGAAATTTATATGGCAATTATACTTTAT TACTTTACATAGAGCTTTGTTTTCAGCTAATATTTTAGAGACAGATTACCCAAAATTACC TAATTTGGTTCCCACTTCATTCCTTCTCAAAAACCAAACATAAAACAGAAGGGGGCCAGC TGTGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGAGGGTGGATCACTAG GTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCCGTCTCTACTAAAAATACA AAAATCACCCAGGTGTGGTGGCACATGCCTGTAATCCCAGATACTTAGGAGGCTGAGGCA GGAGAATCGCTTGAACCCAGGAGACGGAGGTTGCGGTGAGAGCCGAGATCGTGCTACTGC ACTCCAGCCTGGGCAACAGAGCAAGACTCTGTCTCAAAAAAAAAAAAGGAAAGCAGAAGG GGGCATTTTCCTGTCTTCAAAATACAACCCTCTAAGACTAGCAAGTCAGCAGAAACTCAT TGCATTTGGGCTTCTGGGTACTAAATGAATTAAGAGACACCGAGTCCCATCCTTAGATGT CCACATAGATCCCATTGATCAAATTATCCAGTCTTG
Restriction Sites	NotI-NotI
ACCN	BC032998
Insert Size	1296 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032998.2
RefSeq Size	2333 bp
RefSeq ORF	1296 bp
Locus ID	54682
Protein Families	Transmembrane
MW	46.8 kDa

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