Karyopherin beta 3 (IPO5) (BD027479) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD027479, the custom clone sequence may differ by one or more nucleotides CTTCTCTCTCACGCCTAGCGCAATGGCGGCGGCCGCGGCGGASCAGCAACAGTTCTACCTGCTCCTGGGA AACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGCAGAGGAAACCTATGAGAATANTCCCAGGCCAG TCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACAACAGCTGCTGAAGAGGCTAGACAAATGGCCG CCGTTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATGAAGTCTATCCAGCACTTCCCTCTGATGTTCA GACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGATGGAAACACAATCTAGCATGAGGAAAAAAGTT TGTGATATTGCGGSAGAACTGGCCAGGAATTTAATAGATGAGGATGGCAATAACCAGTGGCCCGAAGTTT GAAGTTCCTTTTTGATTCAGTCAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD027479 unedited TGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGCGCCGGCGCCGGCGGCCGC GGCGGGGTGAGAGGCCGCGAGGCCCCGCCCCGTCCTCCCCTTTCCCCTTTGCCCCGCCCT TCCCGCGCGGCCCCCCGCAAGCCCCGCGCCGCCGCTGGTGCCGGTCCCCGCGCTGGGCCC GCCCCCGCCCCTCCCGCGGCCCGCGAGCGCGCCTCACGGCTCCTGTCTCCCCTCCCTCCT TCTCTCTCACGCCTAGCGCAATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGC TCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGCAGAGGAAACCTATG AGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACAACAG CTGCTGAAGAGGCTAGACAAATGGCCGCCGTTCTCCTAAGACGTCTCTTGTCCTCTGCAT TTGATGAAGTCTATCCAGCACTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTAC TCATGATTATTCAGATGGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGG CAGAACTGGCCAGGAATTTAATAGATGAAGGATGGCAATAACCAGTGGCCCGAAGGTTTT GAAGTTCCTTTTTGATTCAGTCAGCTCTCAAATGTGGGACTGCGGGAAGCTGCCCTTCAC ATTNTCTGGGAACTTCCTGGAATTTTTGGGGAACCAGCACACACTATNTTAGAGTCATAA ACGAATGGTANTTCAGTGATGCAAGATCAGGACACCCGTCGATCANGACGTTATCTGCTA AAGCACAGCTGCATTTATACTTGCAAAGAGCATAT
Restriction Sites	NotI-NotI
ACCN	BD027479
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BD027479.1
RefSeq Size	444 bp
RefSeq ORF	444 bp
Locus ID	3843
Protein Families	Druggable Genome
Gene Summary	Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]

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