PTRH2 (AK057033) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BIT1; CFAP37; CGI-147; IMNEPD; PTH; PTH 2; PTH2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105415 sequence for AK057033 edited (data generated by NextGen Sequencing) ATGCCCTCCAAATCCTTGGTTATGGAATATTTGGCTCATCCCAGTACACTCGGCTTGGCT GTTGGAGTTGCTTGTGGCATGTGCCTGGGCTGGAGCCTTCGAGTATGCTTTGGGATGCTC CCCAAAAGCAAGACGAGCAAGACACACACAGATACTGAAAGTGAAGCAAGCATCTTGGGA GACAGCGGGGAGTACAAGATGATTCTTGTGGTTCGAAATGACTTAAAGATGGGAAAAGGG AAAGTGGCTGCCCAGTGCTCTCATGCTGCTGTTTCAGCCTACAAGCAGATTCAAAGAAGA AATCCTGAAATGCTCAAACAATGGGAATACTGTGGCCAGCCCAAGGTGGTGGTCAAAGCT CCTGATGAAGAAACCCTGATTGCATTATTGGCCCATGCAAAAATGCTGGGACTGACTGTA AGTTTAATTCAAGATGCTGGACGTACTCAGATTGCACCAGGCTCTCAAACTGTCCTAGGG ATTGGGCCAGGACCAGCAGACCTAATTGACAAAGTCACTGGTCACCTAAAACTTTACTAG Clone variation with respect to AK057033.1 311 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057033 unedited CTCACTATTAGGGCGGCCGCGAATTCGCACGAGGAGAAGGGAAGGCGCGAGTGAGGAAAG GAGGTACTGTAGCTACACTTNTCTGGAAAATTCAGTATGGACAGTCTCCGACTTAGGATT TTTCAACTTTAGGATGGTGTGAAAGAGACACCCATTCAGTAGAAACTGTACTTCGAGTTT TGCATTTTGATCTTTTCCTGGCCTAGTGATAATGTGGTACAGTACACTCTTGTGATGCTG GGCAGCGGCAGCGAGCCACAGCTCCCAGTCACCCATGTGATCACGGGAATCAACAACCCA TCCTCTACCGTGTACTGTGTTGTCAGCTTTTTTGGATATTGTGTTTTGTGTTTTCACATT CCATCATGTCTACAAAATGTCCATCAGTGTCTCCTGTTTCTGGTGAGATGAAGAAGAGGA AGGCAATTACTCTTGAAATGAAACTCAAGATAATTGCCCAGCATGAAGGTGGCAAGCCAG TAATGGCCATTGCACGTGAGTTAGGACTTTGGCAATCCACGATTTCAACCATCTTAAGGG ATAAGAAGCAAATCAGTGATGCAGCGAAATCGTCAGCATCAGTTAAATCCACTGTCATCA CAAAGAAAAGGGCTGGACCAATTGATGATATGGAAAAATTACTTGTTATGTGGATGGAAG ACCAGATACAGAAGCGTATACCACTTAGCCTACTGATGATCNCAGGCTAAGCAAGAAGTC TTTTTAATATGCTAAAAGACCGTGCCAGTGATCCTACATATACACAAATGTTTAAAGCAG TCATGGATGGTTCCAGCGCTTAAAAGGCGTCATAATTNTCACATGTANAGATCACTGGTG AGGCANCACGTGCTGGTAATGAAGGTGCATAGCTTTAAGGAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057033 unedited TGTACCTCTGCCGCAATCNANATTGAGTTTTTTTCTTTTTTTTTTTGGAACATGGGAATT TGTTTTATTTTCATCTCAAGAACATTTAAGTTGGGTGAAGAAATTCAGCTTTTGTTGTTA GAATCTGACAGGCTTCAAACACTTGTGATGGAGGGGTTGTTGTCATATCAAAGTCCACCT AGTAAAGTTTTAGGTGACCAGTGACTTTGTCTTTTAGGTCTGCTGGTCCTGGCCCAATCC CTAGGACAGTTTGAGAGCCTGGTGCAATCTGAGTACGTCCAGCATCTTGAATTAAACTTA CAGTCAGTCCCAGCATTTTTGCATGGGCCAATAATGCAATCAGGGTTTCTTCATCAGGAG CTTTGACCACCACCTTGGGCTGGCCACAGTATTCCCATTGTTTGAGCATTTCAGGATTTC TTCTTTGAATCTGCTTGTAGGCTGAAACAGCAGCATGAGAGCACTGGGCAGCCACTTTCC CTTTTCCCATCTTTAAGTCATTTCGAACCACAAGAATCATCTTGTACTCCCCGCTGTCTC CCAAGATGCTTGCTTCACTTTCAGTATCTGTGTGTGTCTTGCTCGTCTTGCTTTTGGGGA GCATCCCAAAGCATACTCNGAGGCTCCAGCCCAGGCACATGCCACAAGCAACTCCAACAG CCAAGCCGAGTGTACTGGGATGAGCCAAATATTTCCATACCAAGATTTGAAGGCATCATT AATTTCACAGCTGGAAGCACAGTGTCCTCGAAAATGTTTGAGCCTGAAAAGAATTAATTG CTGATGAAGGGATGCACAAGGCCTCAATTTTCTTCTCTGTGAACATCCAGATAGTTGGTG AGGCTTGGGACTCAAACTTTTGATGGTTACCAGCTGTGTTGTCTCAGGAGTACCAGTTTG CCGGCACCTGGTCTCTTTACTACAATTTTGTGAAGCAACATCTGTTT
Restriction Sites	NotI-NotI
ACCN	AK057033
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK057033.1, BAB71351.2
RefSeq Size	2047 bp
RefSeq ORF	540 bp
Locus ID	51651
Domains	CENPB, CENP-B_N
Protein Families	Transmembrane
Gene Summary	The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

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