YTHDF1 (AK095795) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C20orf21
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105377 sequence for AK095795 edited (data generated by NextGen Sequencing) ATGTCGGCCACCAGCGTGGACACCCAGAGAACAAAAGGACAAGATAATAAAGTACAAAAT GGTTCGTTACATCAGAAGGATACAGTTCATGACAATGACTTTGAGCCCTACCTTACTGGA CAGTCAAATCAGAGTAACAGTTACCCCTCAATGAGCGACCCCTACCTGTCCAGCTATTAC CCGCCGTCCATTGGATTTCCTTACTCCCTCAATGAGGCTCCGTGGTCTACTGCAGGGGAC CCTCCGATTCCATACCTCACCACCTACGGACAGCTCAGTAACGGAGACCATCATTTTATG CACGATGCTGTTTTTGGGCAGCCTGGGGGCCTGGGGAACAACATCTATCAGCACAGGTTC AATTTTTTCCCTGAAAACCCTGCGTTCTCAGCATGGGGGACAAGTGGGTCTCAAGGTCAG CAGACCCAGAGCTCCGCGTATGGGAGCAGCTACACCTACCCCCCGAGCTCCCTGGGTGGC ACGGTGGTTGATGGGCAGCCAGGCTTTCACAGCGACACCCTCAGCAAGGCCCCCGGGATG AACAGCCTGGAGCAGGGCATGGTTGGCCTGAAGATTGGGGACGTCAGCTCCTCCGCCGTC AAGACGGTGGGCTCTGTCGTCAGCAGCGTGGCACTGACTGGTGTCCTTTCTGGCAACGGT GGGACAAATGTGAACATGCCAGTTTCAAAGCCGACCTCGTGGGCTGCCATTGCCAGCAAG CCTGCAAAACCACAGCCTAAAATGAAAACAAAGAGCGGGCCTGTCATGGGGGGTGGGCTG CCCCCTCCACCCATAAAGCATAACATGGACATTGGCACCTGGGATAACAAGGGGCCTGTG CCGAAGGCCCCAGTCCCCCAGCAGGCACCCTCTCCACAGGCTGCCCCACAGCCCCAGCAG GTGGCTCAGCCTCTCCCAGCACAGCCCCCAGCTTTGGCTCAACCGCAGTATCAGAGCCCT CAGCAGCCACCCCAGACCCGCTGGGTTGCCCCACGCAACAGAAACGCGGCGTTTGGGCAG AGCGGAGGGGCTGGCAGCGATAGCAACTCTCCTGGAAACGTCCAGCCTAATTCTGCCCCC AGCGTCGAATCCCACCCCGTCCTTGAAAAACTGAAGGCTGCTCACAGCTACAACCCGAAA GAGTTTGAGTGGAATCTGAAAAGCGGGCGTGTGTTCATCATCAAGAGCTACTCTGAGGAC GACATCCACCGCTCCATTAAGTACTCCATCTGGTGTAGCACAGAGCACGGCAACAAGCGC CTGGACAGCGCCTTCCGCTGCATGAGCAGCAAGGGGCCCGTCTACCTGCTCTTCAGCGTC AATGGGAGTGGGCATTTTTGTGGGGTGGCCGAGATGAAGTCCCCCGTGGACTACGGCACC AGTGCCGGGGTCTGGTCTCAGGACAAGTGGAAGGGGAAGTTTGATGTCCAGTGGATTTTT GTTAAGGATGTACCCAATAACCAGCTCCGGCACATCAGGCTGGAGAATAACGACAACAAA CCGGTCACAAACTCCCGGGACACCCAGGAGGTGCCCTTAGAAAAAGCCAAGCAAGTGCTG AAAATTATCAGTTCCTACAAGCACACAACCTCCATCTTCNACGACTTTGCTCACTACGAG AAGCGCCAGGAGGAGGAGGAGGTGGTGCGCAAGGAACGGCAGAGTCGAAACAAACAATGA Clone variation with respect to AK095795.1 1600 a=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095795 unedited GCACGAGGGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGC AATGGAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC CGGCCCCGCGTCTAGTTGTTCATGAAGCATGTCGGCCACCAGCGTGGACACCCAGAGAAC AAAAGGACAAGATAATAAAGTACAAAATGGTTCGTTACATCAGAAGGATACAGTTCATGA CAATGACTTTGAGCCCTACCTTACTGGACAGTCAAATCAGAGTAACAGTTACCCCTCAAT GAGCGACCCCTACCTGTCCAGCTATTACCCGCCGTCCATTGGATTTCCTTACTCCCTCAA TGAGGCTCCGTGGTCTACTGCAGGGGACCCTCCGATTCCATACCTCACCACCTACGGACA GCTCAGTAACGGAGACCATCATTTTATGCACGATGCTGTTTTTGGGCAGCCTGGGGGCCT GGGGAACAACATCTATCAGCACAGGTTCAATTTTTTCCCTGAAAACCCTGCGTTCTCAGC ATGGGGGACAAGTGGGTCTCAAGGTCAGCAGACCCAGAGCTCCGCGTATGGGAGCAGCTA CACCTACCCCCCGAGCTCCCTGGGTGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095795 unedited NNTTTAGCTTGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCTA AATAGTATTGTTTATTAAGGCTTGTATTCTCCTAGAGGAAAAACCCAATGTCGTCCAGGA CTCCATTCAGGCAGTAACAGTTCAGAGGAAGTGACACGCTCTAAATACAAGTCTAATGAT ACAGCTGAAACGTTAACTCAGAGGGTCTTTTGGAGCAAGTAGTTTTCAGAAAGCGTCTGC TCTCTAGGACGGTAAGGATCCTCTACAAGGGCACGTGCAGATCCAGGCGCTGGAGCGTCA GGCATGGGCACCATTTTCATGCTTCAACTCAAACTCCAGGTGGTAGTGAGCTCAACGGTC CCTCATTCCACAAAACATGACAGCAAATTCATCTTCTAAAAAAAGTTTTGTTTTGTTTTT ACCCATTCAACAGGAAAAAAAATTAGACACACACGATGAAATTTACAACCAGCAGCATCA TCCATCACACTGTCTGTACTACCAGATCCTACACTTAAAGCTCAGCATTATTGGTATAAA AACTTAAGACGGCATTAGAATTCTTAAGAAAAGGTGTAAAATTTAAAAAGATGTGCAAAC AACAAAGAATGCCCGACCCTGAACCAGACCTAAAGCACCTTCCAGTTCCTCCACACATCA TGCCCCAACACCATCCAGCCCAATCGGACACCAGGACAGTGAGGGACGGGTGGCTGTTCA NTGGGCAACAGATCTGGAAGGAAAGATTNTCAAAAAAAAAAAAAGTGTCTGCCAAATTTG AAAATTAAAAAGTATCTGTCTAGCAGAAAATCAAATGGGGTAATTAGCACTTTAGACCGA TNNACAGATATANAATGACCAATCAGAATGGACANNAANTCCTTAACCTTTTTGATTNCA GTATTTNCAAAGAGATNNTATCAGAATACTCTAAATGACAAAATGACTCATGGG
Restriction Sites	NotI-NotI
ACCN	AK095795
Insert Size	3110 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095795.1, BAG53130.1
RefSeq Size	2967 bp
RefSeq ORF	2967 bp
Locus ID	54915
Domains	YTH
Gene Summary	Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity).[UniProtKB/Swiss-Prot Function]

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