RBM25 (AK026107) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	fSAP94; NET52; RED120; RNPC7; S164; Snu71
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026107, the custom clone sequence may differ by one or more nucleotides AAGACCTCCATCAGCTCGCCGCGCAGCGCGGCTGTATTTGCGGCCTGTGCGAGTAGGCGCTTGGGCACTC AGTCTCCCTGGCGAGCGACGGGCAGAAATCTCGAACCAGTGGAGCGCACTCGTAACCTGGATCCCAGAAG GTCGCGAAGGCAGTACCGTTTCCTCAGCGGCGGACTGCTGCAGTAAGAATGTCTTTTCCACCTCATTTGA ATCGCCCTCCCATGGGAATCCCAGCACTCCCACCAGGGATCCCACCCCCGCAGTTTCCAGGATTTCCTCC ACCTGTACCTCCAGGGACCCCAATGATTCCTGTACCAATGAGCATTATGGCTCCTGCTCCAACTGTCTTA GTACCCACTGTGTCTATGGTTGGAAAGCATTTGGGCGCAAGAAAGGATCATCCAGGCTTAAAGGCTAAAG AAAATGATGAAAATTGTGGTCCTACTACCACTGTTTTTGTTGGCAACATTTCCGAGAAAGCTTCAGACAT GCTTATAAGACAACTCTTAGCTAAATGTGGTTTGGTTTTGAGCTGGAAGAGAGTACAAGGTGCTTCCGGA AAGCTTCAAGCCTTCGGATTCTGTGAGTACAAGGAGCCAGAATCTACCCTCCGTGCACTCAGATTATTAC ATGACCTGCAAATTGGAGAGAAAAAGCTACTCGTTAAAGTTGATGCAAAGACAAAGGCACAGCTGGATGA ATGGAAAGCAAAGAAGAAAGCTTCTAATGGGAATGCAAGGCCAGAAACTGTCACTAATGACGATGAAGAA GCCTTGGATGAAGAAACAAAGAGGAGAGATCAGATGATTAAAGGGGCTATTGAAGTTTTAATTCGTGAAT ACTCCAGTGAGCTAAATGCCCCCTCACAGGAATCTGATTCTCACCCCAGGAAGAAGAAGAAGGAAAAGAA GGAGGACATTTTCCGCAGATTTCCAGTGGCCCCACTGATCCCTTATCCACTCATCACTAAGGAGGATATA AATGCTATAGAAATGGAAGAAGACAAAAGAGACCTGATATCTCGAGAGATCAGCAAATTCAGAGACACAC ATAAGAAACTGGAAGAAGAGAAAGGCAAAAAGGAAAAAGAAAGACAGGAAATTGAGAAAGAACGGAGAGA AAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGAAAGGGAAAGAGAACGTGAA CGAGAAAAGGAGAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGACCGTGACCGGACAAAAGAGAGAG ACCGAGATCGGGATCGAGAGAGAGATCGTGACCGGGATAGAGAAAGGAGCTCAGATCGTAATAAGGATCG CAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGAGAGCGGGAAAGAGAGAGAGAGAGAGAA CGAGAGCGAGAACGAGAACGGGAGCGAGAGAGAGAGCGAGAGAGGGAACGGGAGCGAGAAAGAGAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026107 unedited GAGTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTCGCCG CGCTGCGCGGCTGTATTTGCGGCCTGTGCGAGTAGGCGCTTGGGCACTCAGTCTCCCTGG CGAGCGACGGGCAGAAATCTCGAACCAGTGGAGCGCACTCGTAACCTGGATCCCAGAAGG TCGCGAAGGCAGTACCGTTTCCTCAGCGGCGGACTGCTGCAGTAAGAATGTCTTTTCCAC CTCATTTGAATCGCCCTCCCATGGGAATCCCAGCACTCCCACCAGGGATCCCACCCCCGC AGTTTCCAGGATTTCCTCCACCTGTACCTCCAGGGACCCCAATGATTCCTGTACCAATGA GCATTATGGCTCCTGCTCCAACTGTCTTAGTACCCACTGTGTCTATGGTTGGAAAGCATT TGGGCGCAAGAAAGGATCATCCAGGCTTAAAGGCTAAAGAAAATGATGAAAATTGTGGTC CTACTACCACTGTTTTTGTTGGCAACATTTCCGAGAAAGCTTCAGACATGCTTTATAGAC AACTCTTAGCTAANTGTGGTTTGGGTTTGAGCTGGAAGAGAGTACANGGTGCTTCCGGAA AGCTTCAAGCCTTCGGATTCTGTGAGTACAAGGAGCCAGAATCTANCCCTCGTGCACTCA GATTATTACATGACCTGGCAAATTGGAGAGAAAAAGCTACTCGTTTAAAGTTGATGCAAA GAACAAAAGCCCCAGCTGGGATGAATGGGAAGCCAAANAAAAAAGCTTCTAATGGGGAAT GCAAGGNCCANAAACTGTTCCTAATTGACCATGAAAAAACCCTTGGGTTGAAAAAACAAA GGGGAAAGTTCCGATGATTTAAGGGGGCTATTGAAGTTTTAATTCGGGAAAACCCCAGTG GACCTAAAGGCCCCCTCCAGGAATCTGATTTTCACCCCCA
Restriction Sites	NotI-NotI
ACCN	AK026107
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026107.1, BAB15362.1
RefSeq Size	1480 bp
RefSeq ORF	1480 bp
Locus ID	58517
Domains	RRM
Protein Pathways	Spliceosome
Gene Summary	RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.[UniProtKB/Swiss-Prot Function]

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