MGA (BD078427) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD078427, the custom clone sequence may differ by one or more nucleotides GAATTCGGCACGAGTTTACATCAGAAAAGAGCTGGAAGTCTTCCTGCAATGAAGGAGAATCCTCTTCTAC TTCTTATATGCATCARAGGTCACCTGGTGGTCCCACCAAACTGATTGAGATCATCTCAGACTGCAACTGG GAGGAAGATCGGAACAAGATTTTRAGCATCTTATCCCAGCACATCAATAGCAACATGCCACAATCACTTA AGGTGGGCAGCTTCATCATTGAGTTGGCTTCTCAGCGAAAGAGCCGGGGTGAGAAGAACCCTCCTGTTTA TTCTTCTCGTGTGAMAATCTCTATGCCATCATGTCAAGACCAAGATGATATGGCTGAGAAATCTGGATCA GAGACTCCTGATGGTCCATTGTCCCCTGGGAAAATGGAGGATATCTCTCCTGTGCAGACAGATGCCCTGG ATTCAGTGAGGGAGAGATTACATGGAGGCAAAGGTCTGCCTTTTTATGCAGGGCTTTCTCCTGCAGGGAA GCTTGTGGCCTATAAACGTAAACCCAGTTCAAGTACATCTGGGCTTATCCAGGTGAGAATTATCTTTAAT CTGGGTATAGCACCTTTGTATACACCTAGGTAGTATCATGATTTTTCAGAGCCCTTTATGGTCCTGATAT CCTTTATCTTGACATTTCCTGGGAACTGGGTGACAAAATTATTATCTCTTTTTGTAATAGGCCTAGTTTA GATGCATACCTAGAGTGAATTTTTGTCACATTTATGAACAGAAACGTAGAGCCTTGTATTAGTTTTAATT TTCTTTCTAATCTTCCCAGAAAGTTGCTCTTCATAAACTTTATTGCCTGCAGGCTCTAGTGATACTTTGA CAATAAAGCAAGGGTAATCAGGGATTCAGTCTAGCTCTTGGAATTTATTATTAGCAGATAGGTTTCAAAA CAAAACCATGGTTAGAACGGTAGGTGTAAGGGGAAGATGAAATTGACTTAAAGATAGGCAATATATGTTT AGAAACTTGGGGAAAAAAAAAAAAAAAAACTCGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD078427 unedited CTATAGGGCGGCCGCGAATTCGGCACGAGGAAAGGAACCTGATTCTGAACAGCAGCCCTT AAAACAACTCACCTGTGATTTGGAGGATGATTCTGATAAATTACAAGAAAAGAGCTGGAA GTCTTCCTGCAATGAAGGAGAATCCTCTTCTACTTCTTATATGCATCAGAGGTCACCTGG TGGTCCCACCAAACTGATTGAGATCATCTCAGACTGCAACTGGGAGGAAGATCGGAACAA GATTTTGAGCATCTTATCCCAGCACATCAATAGCAACATGCCACAATCACTTAAGGTGGG CAGCTTCATCATTGAGTTGGCTTCTCAGCGAAAGAGCCGGGGTGAGAAGAACCCTCCTGT TTATTCTTCTCGTGTGAAAATCTCTATGCCATCATGTCAAGACCAAGATGATATGGCTGA GAAATCTGGATCAGAGACTCCTGATGGTCCATTGTCCCCTGGGAAAATGGAGGATATCTC TCCTGTGCAGACAGATGCCCTGGATTCAGTGAGGGAGAGATTACATGGAGGCAAAGGTCT GCCTTTTTATGCAGGGCTTTCTCCTGCGGGAAGCTTGTGGCCTATAAACGTAAACCCAGT TCAAGTACATCTGGGCTTATCCAGGTTAATGGTAAGAGTTACCCGCAGGCTAAGTTGCTA TTGGGACAGATGGGGGCATTGCATCCAGCCAATAGGCTAGCTGCTTATATCACAGGCAGG TNGCGACCCTCAGTCCTGGACCTCTCAACCCTCAGTACTGTCATCTNCAAGGTAGCATCC ATGGCCAGGNTGGNCTGCATCAGAAACACGTACCCTGNTGCCTTCACATNNCATNCAAAT GGCATNNCTCTCTGGCCTGCACAAATCGCCTGGGAGATCTGAAGGCCGTTGTCAGCAAAC GGNCATG
Restriction Sites	NotI-NotI
ACCN	BD078427
Insert Size	4500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD078427.1
RefSeq Size	1014 bp
RefSeq ORF	1014 bp
Locus ID	23269
Gene Summary	Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor (By similarity).[UniProtKB/Swiss-Prot Function]

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