CHST5 (AK025820) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALYE870; JBTS20; MKS11; PRO1886
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105315 sequence for AK025820 edited (data generated by NextGen Sequencing) ATGGCGCTCTATGAGCTCTTCTCTCACCCGGTCGAGCGCAGTTACCGCGCGGGGCTCTGC TCCAAAGCCGCGCTGTTCCTGCTGCTGGCCGCTGCGCTCACGTACATCCCGCCGCTGCTG GTGGCCTTCCGGAGCCACGGGTTTTGGCTGAAGCGGAGCAGCTACGAGGAGCAGCCGACC GTGCGCTTCCAACACCAGGTGCTGCTCGTGGCCCTGCTCGGACCCGAAAGCGACGGGTTC CTCGCCTGGAGCACGTTCCCCGCCTTCAACCGGCTGCAAGGGGATCGCCTGCGCGTCCCG CTCGTTTCGACTAGAGAAGAAGACAGGAACCAGGATGGGAAGACGGACATGTTACATTTT AAGCTGGAGCTTCCCCTGCAGTCCACGGAGCACGTTCTCGGTGTGCAGCTCATCCTGACT TTCTCCTATCGATTACACAGGATGGCGACCCTCGTGATGCAGAGCATGGCGTTTCTCCAG TCCTCCTTTCCTGTCCCGGGATCCCAGTTATACGTGAACGGAGACCTGAGGCTGCAGCAG AAGCAGCCGCTGAGCTGTGGTGGCCTAGATGCCCGATACAACATATCCGTGATCAACGGG ACCAGCCCCTTTGCCTATGACTACGACCTCACCCATATTGTTGCTGCCTACCAGGAGAGG AACGTTACCACCGTCCTGAATGATCCCAACCCCATCTGGCTGGTGGGCAGGGCCGCAGAT GCTCCATTTGTGATTAATGCTATCATCCGATACCCTGTGGAAGTCATTTCTTATCAGCCA GGATTCTGGGAGATGGTAAAGTTCGCCTGGGTGCAGTATGTCAGCATCCTGCTTATCTTC CTCTGGGTGTTTGAAAGAATCAAGATCTTCGTGTTTCAGAATCAGGTGGTGACCACCATT CCTGTGACAGTGACGCCCCGGGGAGACTTGTGTAAGGAGCACTTATCCTAG Clone variation with respect to AK025820.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK025820 unedited NTTTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG CCGCGAATTCGGCACGAGGGCCAAACTTGGTCCCCCGGCTCGCGGAGTGCCTGCGAGCGG TGCTCATGGCGCTCTATGAGCTCTTCTCTCACCCGGTCGAGCGCAGTTACCGCGCGGGGC TCTGCTCCAAAGCCGCGCTGTTCCTGCTGCTGGCCGCTGCGCTCACGTACATCCCGCCGC TGCTGGTGGCCTTCCGGAGCCACGGGTTTTGGCTGAAGCGGAGCAGCTACGAGGAGCAGC CGACCGTGCGCTTCCAACACCAGGTGCTGCTCGTGGCCCTGCTCGGACCCGAAAGCGACG GGTTCCTCGCCTGGAGCACGTTCCCCGCCTTCAACCGGCTGCAAGGGGATCGCCTGCGCG TCCCGCTCGTTTCGACTAGAGAAGAAGACAGGAACCAGGATGGGAAGACGGACATGTTAC ATTTTAAGCTGGAGCTTCCCCTGCAGTCCACGGAGCACGTTCTCGGTGTGCAGCTCATCC TGACTTTCTCCTATCGATTACACAGGATGGCGACCCTCGTGATGCAGAGCATGGCGTTTC TCCAGTCCTCCTTTCCTGTCCCGGGATCCCAGTTATACGTGAACGGAGACCTGAGGCTGC AGCAGAAGCAGCCGCTGAGCTGTGGTGGCCTAGATGCCCGATACACATATCCGTGATCAA CGGGACCAGCCCCTTTGCCTATGACTACGACCTCACCCATATTGNTGCTGCCTACCAGAG AGGAAACGTACCACCGTCCTGAATGATCCCACCCNATCTGGCTGTGGGCAGGCCCGCAGA GCTCCATTTGTGATAATGCTATATCCGATACCTGTGAAGTCATTNCTGTCTN
Restriction Sites	NotI-NotI
ACCN	AK025820
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK025820.1, BAB15244.1
RefSeq Size	2708 bp
RefSeq ORF	951 bp
Locus ID	23563
Gene Summary	The protein encoded by this gene belongs to the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family, members of which catalyze the transfer of sulfate to position 6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) residues within proteoglycans, and sulfation of O-linked sugars of mucin-type acceptors. Carbohydrate sulfation plays a critical role in many biologic processes. This gene is predominantly expressed in colon and small intestine. [provided by RefSeq, Aug 2011]

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