UBE2D2 (AK001428) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4; UBCH5B
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105115 sequence for AK001428 edited (data generated by NextGen Sequencing) ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAG TGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCA AATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTAC CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT AATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCA AAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTG CCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAA TGGACTCAGAAGTATGCGATGTAA Clone variation with respect to AK001428.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK001428 unedited TGGATTTTGTAATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGAGCAGGGAGG AAGACAGGCAATCCCTCCGGCTGTCCGACCAAGAGAGGCCGGCCGAGCCCGAGGCTTGGG CTTTTGCTTTCTGGCGGAGGGATCTGCGGCGGTTTAGGAGGCGGCGCTGATCCTGGGAGG AAGAGGCAGCTACGGCGGCGGCGGCGGTGGCGGCTAGGGCGGCGGCGAATAAAGGGGCCG CCGCCGGGTGATGCGGTGACCGCTGCGGCAGGCCCAGGAGCTGAGTGGGCCCCGGCCCTC AGCCCGTCCCGCCGGACCCGCTTTCCTCAACTCTCCATCTTCTCCTGCCGACCGAGATCG CCGAGGCGGCCTCAGGCTCCCTAGCCCCTTCCCCGTCCCTTCCCCGCCCCCGTCCCCGCC CCGGGGGCCGCCGCCACCCGCCTCCCACCATGGCTCTGAAGAGAATCCACAAGGAATTGA ATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGT TCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTT TCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAA CAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGAT CACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGT GTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTTGCTCGATCTNCAAACAGATGA GAAAAGTCCACAGATAGCTCGGGAATGACTCAGAGTATGCGATGTATTAAAGAAATATGN ATACCTCTACAATAAGATAGGGGACTCTGAAGAGAAGTCCTTTGATTCCATTGACTGCTT CTTGAGCCCC
Restriction Sites	NotI-NotI
ACCN	AK001428
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK001428.1, BAG50911.1
RefSeq Size	2526 bp
RefSeq ORF	2526 bp
Locus ID	7322
Domains	UBCc
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

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