MYO9B (AK002201) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CELIAC4; MYR5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK002201, the custom clone sequence may differ by one or more nucleotides ATGTCACCTGGGGCGCTGGCCATTATCTTCGCACCCTGCCTCCTGCGCTGCCCTGACAACTCGGACCCGC TGACCAGCATGAAGGACGTCCTCAAGATCACCACGTGCGTGGAGATGCTGATCAAGGAGCAGATGAGGAA ATACAAAGTGAAGATGGAGGAGATCAGCCAACTGGAGGCTGCAGAGAGTATCGCCTTCCGCAGGCTTTCG CTCCTGCGACAAAATGCTCCATGGCCTCTCAAACTGGGGTTTTCGTCTCCCTATGAGGGGGTCCTGAACA AGAGCCCCAAGACCCGGGACATCCAGGAGGAGGAGCCGGAGGTGCTGCTGGAGGAGGAGGCAGCCGGCGG CGATGAGGACCGGGAAAAGGAGATTCTCATTGAACGGATCCAGTCCATCAAGGAGGAGAAGGAGGACATC ACCTACCGGCTGCCGGAGCTGGACCCAAGGGGCTCGGACGAGGAGAACCTGGACTCGGAGACGTCGGCCA GCACCGAGAGCCTGCTGGAGGAGCGGGCCGGGCGGGGGGCCTCGGAAGGTCAGTATTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK002201 unedited NGGGGTCACATATTTGTATACGACTCATATAGGCGGCCGCGCGCAGTCGGGTGCTGCCAA CCGCACTCGGGAGCTCCGGCAGGCGCTGCAGACAGACCCCGCAGCAGTCAAGCTGGAGAA CTTCCCCATCCACGCCATCACAGGGGTGCTGAAGCAGTGGCTGCGGGAGCTGCCCGAGCC CCTCATGACCTTCGCACAGTACGGCGACTTCCTCCGAGCCGTCGAGCTGCCGGAGAAGCA GGAGCAGCTGGCTGCCATCTATGCCGTCCTGGAGCACCTTCCAGAAGCCAACCACAACTC CCTGGAGAGACTCATCTTCCACCTTGTCAAGGTGGCCCTGCTCGAGGATGTCAACCGCAT GTCACCTGGGGCGCTGGCCATTATCTTCGCACCCTGCCTCCTGCGCTGCCCTGACAACTC GGACCCGCTGACCAGCATGAAGGACGTCCTCAAGATCACCACGTGCGTGGAGATGCTGAT CAAGGAGCAGATGAGGAAATACAAAGTGAAGATGGAGGAGATCAGCCAACTGGAGGCTGC AGAGAGTATCGCCTTCCGCAGGCTTTCGCTCCTGCGACAAAATGCTAACAAGAGCCCCAA GACCCGGGACATCCAGGAGGAGGAGCTGGAGGTGCTGCTGGAGGAGGAGGCAGCCGGCGG CGATGAGGACCGGGAAAAGGAGATTCTCATTGAACGGATCCAGTCCATCAAGGAGGAGAA GGAGGACATCACCTACCGGCTGCCGGAGCTGGACCCANGGNGCTCGGACGAGGAGAACCT GGACTCGGAGACGTCGGCCAGCACCGAAAGCCTGCTGGAGAAGCGGCCCGGCGGGGGGCC TCCGAAAGTCAGTATTAAAGGCCCCCTGGGGCTGGTCTCCCTTGCCCCGGCGCGCCCAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK002201 unedited NCCGTCTTCTATGTACGCGGCCGCTTTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCATTAAGGCTGTTACAGTTTAAATTTCC TTAAACCACGTCCAAAAAAACATTTAAACGTACAAAAAGGGGATTGGGGAAATTTAAGGC CCCAGGTGGAAATTTGGGGCTCCCCACAGGTGCCTCCATTATTTGGGGGCTGCGTGCAAG GGGGCCTGGTTCCAACCGGCCTTTCCCTGCCTTGATTTTGCATTTGGGGCCGGGGCCCAC CTAACGTAACTTTTGAGCTTTTGATTCTGGATCCTTTTTTGAGGGCCAAACACTATTTTT GAAGTTTTGGCCCCCATTTCCAGTGCAGGGGCCGTCCTCGGACATGCAAATTTTGTCAGC TGTGGCTTTAACCCTTTGGTCTGGCCCGAGGCGGGGGGCAGGCATTACTTTGGGGGATCC AATTACCTCCGTTTGGCCCCAGGTGGCTGGCCATCCTTTTTTAGGGGCTCCAGGGCCCCC TGCACTGGCAGGTCTGCCCCGGGCGTGATGTACACGGAATGTTTTTGGTCCGGGCGGCGG GCAGGTGGCTCCCGGCGCCGCATTGGGGCATCCGCCTCCCGGGCACCCGGTGCCCATCGA GGCAGGTGGAAGGGCAGGCCCTGGGGGAACCTTGATGTTTGGCCGTGGGCATAATGGGGG GTCGCCGGGGGGTCTCCACTCTGACCGTTACAAAGACAACGGCCTTCGTCTTGGAGGGCC GCCCCCGGGGGGGGAGGGGCCTCGGGGGGGGGCCCCCCGGGCCAGGGGAAAACAGCCCCA GGGGCCCTTTATTCTTGACTTCCGAAGCCCC
Restriction Sites	NotI-NotI
ACCN	AK002201
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK002201.1, BAA92132.1
RefSeq Size	2087 bp
RefSeq ORF	549 bp
Locus ID	4650
Gene Summary	This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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