NDUFS8 (AK002110) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CI-23k; CI23KD; TYKY
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK002110, the custom clone sequence may differ by one or more nucleotides AAGTAGCGGCGGCGCTTCAAGATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTG CACGTGCAGGACCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAGTGAGTACCT GGGTGGCCTCTGGCCTCAGGTGTTCCTGACTGGTCCTGCTGGAGTAGGGGAAGAGACCTGAGTTCCAGTC CTGGCTTTGTCAGGGGCGTTCTTCTCTGGGCCTGTTTCCACTTTTAAAATGATTATGGTGATGGAGACTG ACTCTGATTCACAGGGGCAGGGTCCATCATTGCCGAGGTTAGCATGTGTGATCTCAGAGCTCCCTGGGTG TGATGGGGAGGGGGTTGCCAGGTGTGAGCCATGGGTACCTGTGCCTATTGCCAGTCCCTGGTGAAGGCAA AGGCTCAAGCCCCCCACCTACCCCCCTTGATTCCTCTTTCAAGCCCTGTTGGGCAGGGAAGTGCGTCTGG GCTCCAATGGCCACCCAAGCACAGGGAGAGGTAGGCACGGACGGGCTCAGGCCAGAGGCGGCCTCCAGGG TCCTTGCTGAGGCTGGGGAGAGGTGTGGTGAGTGTACAGCCCCTTTGTAGCCCAGCAGTTGCACCTGGAA GTGAGGGCCAGGCTGCTCTCCCTGAGGCTCCAGGGAGACAGTGTGTGAGGCCTCTTGCCATGGCCTCCCT GCCCGCCTCTCTGCAGAGTATGTGAACATGCAGGATCCCGAGATGGACATGAAGTCAGTGACTGACCGGG CAGCCCGCACCCTGCTGTGGACTGAGCTCTTCCGAGGTGCGTCCTGGGCAGGAGGGGACAGGGAAGGTGC CGGGTGGGCACGGATGCATGGGGGAGGAGGGTGCCCCTGCCCACCACACCCGTGCTGCCCACAGGCCTGG GCATGACCCTGAGCTACCTGTTCCGGGAACCGGCCACCATCAACTACCCGTTCGAGAAGGGCCCGCTGAG CCCTCGCTTCCGTGGGGAGCATGCGCTGCGCCGGTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAG CTCTGCGAGGCCATCTGCCCCGCCCAGGTGAGCCCCTGCCCCAACCGGCCACCACGCCAGCCCCTGCCAC TGGGAGAGGGAGGCCAGGCAGCCCTAGGCCCAAACCCTAGCCCCTGCTTGATGTGCGTCCCCAGGAAGTC CCTTTCCCCCTCTGAGCCACTTCCCTCGTGAATGGGAATGATGAGGGCTTGTTACCAGAGCACAGAGTCA GTGAGTTGGCAGAGTGAAGCTTCCAGCCCTGGGCAGACGCCTGACACACAGAGGTTCCCCCTTGGGGTCT GGCCAAGGGTGTGACCCCTGGGCCTCTGAGCCATGGGGTGACACCTGAGGCCATCCACGCCTCCCTGGAT GTAGCCACCGTTTCCGGAGTCCTGCTTGTGTGCCTGTTGCAGCTTCCCTTCCCCAGTGGCTGCTCCCCTG AGGCCCGGCTTTCCGTTCTCTTAGTGGGTCTCGTGCTTACCCGTGGGGGCCAGGCCTGCCAGCACGGGAA CAATATGACTCGGTCAGAGCTCTGGTCAGACCCCTGTGTGTGCCTGGCAGGAGCCCCGGCAGCCGCCGTC GCTACTTTCTGTGTGCACAGCAACCCCTCCCAGTGTTCTGTGACTCGGGTGGCTCCTGTCCAGATCCTAG TTTGTCCTCTGGAGCACTCCCAGGTCCTCCAGGCCATGAGCTGCGGCCCTGATGTCTCTACTCCAGCCAC GGACTGAGAGTGCATAGGAGTGTCCCCAAACAGCAGTCACGATGGTCACAACCTTGGCTTCCAGACAGTG AGTGCTGTCCACAGACAGACCCGTTGCTGTGCCATGTGTGTGACTCACAGCCTCTCCTGTAATCTCCACA ACCCCTCGAGGAGGTCGAGGTTATTGTCTCCATCTTTATAAGCACTAAAGGATGAGCCTGCCAGCCACAG GTCCCACTGTGAGTTGTGGACACTACATTTCTGGGATGGGGGCATATGAGAGTCCTTGCCAAGCACATTT GAAGGTTTTCCCAGCCAGCCCCTCCCCCAGCGGGCTACCCAGGAACTGAGAGTTAGGACGTGCCCTTGAG TTTGGGCTGTGCCCCCACTTGCTGTGTAGCTTTGGGGAGCCCCTCAACCTCTCGGAACCTTGTTTTTCTC ATCCATCAGATGGAGAGAAGTAGATTTAAGGCACAGGATTGCACCCAACAGTGTAGGCATTCTTGGCGAT GTTGCAGGTTCGGCTCCAGACCATCGGGGTAAACTGAGTCACACAGCTGTTTTGGTTTTCCAGTGCATTT AAAAGTTAGATTTAGACCGGGCACAGTGGCTCATGCCTGTAATTCCAGCACTTTGAGAAGCCGAGGTGGG CGGATCACTTGAGGTCAAGAGTTCGAGACCAGCCTGGCCAACATAGTGAAACCCCGTCTCTACTAAAAAT AACAAAACTTAGCCGGGCACGTTGGCAGGTCCCTGTAATCTCAGCTACTTGCTAGGCTGAGGCACGATAA TCACTTGAACCGGGAGGTGGGGGTTGCAGTGAGCCAAAATTGAAAAAAAAAAAAAATTAGGGCCGGTTGC GGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCGGATCACAAGGTCAGGAATTCAA GACCAGCCTGGCCAACATGTTGAAACCCCGTCTCTAGTAAAAATACAAAAATTAGCCGGGTGTGGGGTTG CACACCTGTAGTCCCAGCTACTCAAGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTG CAGTGAACTATGATTGCACCACCATACTCCATCCTGGGCCACAGAGCAAGACTTCGTCTCAAAAAAAAAA AAGGATTTACACTGTACTGTAGGCTGCGAAGTGTCTAAAACACAATGTATATACCTTAATTAAAAATACT TTAGGGGCCAGGCACAGTGGCTCACACCTGTAGTCCAAGTGCTTTGGGAGGTCAAGGTGAGGGGATTACT TGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGCAAAACCCCGACTCTACAACAACAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK002110 unedited CCCCCTTCNGGATTACGTATACGATTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCA AGGCAAGTAGCGGCGGCGCTTCAAGATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGC CCTGGCCCAGGCTGCACGTGCAGGACCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGT GGCAGCCACCTACAGTGAGTACCTGGGTGGCCTCTAGCCTCAGGTGTTCCTGACTGGTCC TGCTGGAGTAGGGGAAGAGACCTGAGTTCCAGTCCTGGCTTTGTCAGGGGCGTTCTTCTC TGAGCCTGTTTCCACTTTTAAAATGATTATGGTGATGGAGACTGACTCTGATTCACAGGG GCAGGGTCCATCATTGCCGAGGTTAGCATGTGTGATCTCAGAGCTCCCTGGGTGTGATGG GGAGNGGGGTTGCCAGGTGTGAGCCATGGGTACCTGTGCCTATTGCCAGTCCCTGGTGAA GGCAAAGGCTCAAGCCCCCCACCTACCCCCCTTGATTCCTCTTTCAAGCCCTGTTGGGCA GGGAAGTGCGTCTGGGCTCCAATGGCCACCCAAGCACAGGGAGAGGTAGGCACGGACGGG CTCAGGCCAGAGGCGGCCTCCAGGGTCCTTGCTGAGGCTGGGGAGAGGTGTGGTTGAGTG TACAGCCCCTTTGTACCCCAGCAGTTGCACCTGGAAGTGAGGGCCAGGCTGCTCTCCCTG AGGCTCCAGGGAGACAGTGTTGTGCAGGCCTCTTTGCCATGGCCTCACTTGCCCGCCTCT CTGCAGAGTATGTGAACATGCCANGATCCCTGAGATGGACCATGAAATCACTGACTTGAC CGGGCAGCCCCGCACCCTGCTGGGGGACTGAGCTCTTTCCGAGGCCTGGGCATGCACTCT GAGCTACCTTGNTTCCGGGAACCCGCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK002110 unedited NGGCCTCCTTAGNACCGCGGCCGCATTCTAAGATCGGTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTAACAGGGGGACCTGGGGCTGGCAGGCTCATCCT TTAGGGCTTATAAAGATGGAAACAATAACCTCGACCTCCTCGAGGGGTTGGGGAAATTAC AGGAAAGGCTGTGAGTCACACACATGGCACAACAACGGGTCTGTCTGTGGACAGCACTCA CTGTCTGGAAGCCAAGGTTGGGACCATCGGGACTGCTGTTTGGGGACACTCCTATGCACT CTCATTCCGTGGCTGGAGTAAAAACATCAGGGCCGCAGCTCATGGCCTGGAGGACCTGGG AGTGCTCCAAAGGACAAACTAGGATCTGGACAGGAGCCACCCGAGTCACAAAACACTGGG AGGGGTTGCTGTGCACACAAAAAGTANCGACGGCGGCTGCCGGGGCTCCTGCCAGGCACA CACAGGGGTCTGACCAAAGCTCTGACCGAGTCATATTGTTCCCGTGCTGGCAGGCCTGGC CCCCACGGGTAAGCACGAGACCCACTAATATAACGGAAAGCCGGGCCTCAGGGGAGCAGC CACTGGGGAAGGGAAGCTGCAACAGGCACACAAGCAGGACTCCGGAAACGGTGGCTACAT CCAGGGAGGCGTGGATGGCCCTCAGTGTCACCCCATGGCTCAAAGGCCCCAGGGTCAACC CTTGGCCAGACCCNAGGGGGAACCTCTGGGTGTTAGGCGCCTGCCCAGGGCTGGAAGCTT ACTCTGCCCAATCACTGACTTTGGGCTCTGGGAAACAGCCCTCATAATTCCCATTCACGA GGAAGTGGCCCCAAAAGGGGGAAAGAGGACTTCCCCGGGGC
Restriction Sites	NotI-NotI
ACCN	AK002110
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK002110.1
RefSeq Size	3073 bp
RefSeq ORF	3073 bp
Locus ID	4728
Domains	fer4
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary	This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]

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