myosin heavy chain 9 (MYH9) (AK025393) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BDPLT6; DFNA17; EPSTS; FTNS; MHA; NMHC-II-A; NMMHC-IIA; NMMHCA
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK025393, the custom clone sequence may differ by one or more nucleotides AGTCAGTATTGTATATGAATTTTCTGCCATGTGGTAGAAAGAGGGTGCCTGTCTTTGTACTCTGTGTGAA CAGGCACACCTGTTTACCTGTCTTGGTTTCACCGGTTTTATAGGAGTTGCAACAGAGGATGGCATTTTAC ACTTACTTTTGTGCCTTTCCTGTTGTAATTGGTCCTGTTTGCCTGAGCTGTTTTTGTGCTACTGTTGTGT GTGAAGATGTCCGACACCTAAACATTTTGCGGGAAGCTTTGCCTGCTTTCCTGAGAGTGGTCCTGTCTAG CTCAGCTGTGGCTCCCAAGACTCCACTTTGCTTCTTTCTGGTGGGAGCAGCCAGGCACCTGGTTTCCCTC TCGAATTCACTTTGAGATGTGTGGGCTGTGCTGTGGCCGCCCCTGGCCTGGCCGGGCCCGCTGACCCTCT GCTTCTACCTCTGCCCCCAAGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCG CCGGGGATGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATAAGC CTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCCAGACCCCGCAGCA CGCCTCTCCCCACCTTCTTGGGACTGCTATGAACATGCCTCCTCCTGCCCTCCGCCCCGTCCCCCCATCC CGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTCCTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTG CTCAGAATCTGATACCAAAGAGACAGGGCCCGGGCCCAGGCAGAGAGCGACCAGCAGGCTCCTCAGCCCT CTCTTGCCAAAAAGCACAAGATGTTGAGGCGAGCAGGGCAGGCCCCCGGGGAGGGGCCAGAGTTTTCTAT GAATCTATTTTTCTTCAGACTGAGGCCTTTTGGTAGTCGGAGCCCCCGCAGTCGTCAGCCTCCCTGACGT CTGCCACCAGCGCCCCCACTCCTCCTCCTTTCTTTGCTGTTTGCAATCACACGTGGTGACCTCACACACC TCTGCCCCTTGGGCCTCCCACTCCCATGGCTCTGGGCGGTCCAGAAGGAGCAGGCCCTGGGCCTCCACCT CTGTGCAGGGCACAGAAGGCTGGGGTGGGGGGAGGAGTGGATTCCTCCCCACCCTGTCCCAGGCAGCGCC ACTGTCCGCTGTCTCCCTCCTGATTCTAAAATGTCTCAAGTGCAATGCCCCCTCCCCTCCTTTACCGAGG ACAGCCTGCCTCTGCCACAGCAAGGCTGTCGGGGTCAAGCTGGAAAGGCCAGCAGCCTTCCAGTGGCTTC TCCCAACACTCTTGGGGACCAAATATATTTAATGGTTAAGGGACTTGTCCCAAGTCTGACAGCCAGAGCG TTAGAGGGGCCAGCGGCCCTCCCAGGCGATCTTGTGTCTACTCTAGGACTGGGCCCGAGGGTGGTTTACC TGCACCGTTGACTCAGTATAGTTTAAAAATCTGCCACCTGCACAGGTATTTTTGAAAGCAAAATAAGGTT TTCTTTTTTCCCCTTTCTTGTAATAAATGATAAAATTCCGAGTCTTTCTCACTGCCTTTGTTTAGAAGAG AGTAGCTCGTCCTCACTGGTCTACACTGGTTGCCGAATTTACTTGTATTCCTAACTGTTTTGTATATGCT GCATTGAGACTTACGGCAAGAAGGCATTTTTTTTTTTTTAAAGGAAACAAACTCTCAAATCATGAAGTGA TATAAAAGCTGCATATGCCTACAAAGCTCTGAATTCAGGTCCCAGTTGCTGTCACAAAGGAGTGAGTGAA ACTCCCACCCTACCCCCTTTTTTATATAATAAAAGTGCCTTAGCATGTGTTGCAGCTGTCACCACTACAG TAATCTGGTTTACAGATGTTTTCCACTGAGCATCACAATAAAGAGAACCATGTGCTAAAAAAAAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK025393 unedited CCCAGCTCGTTTTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACCAGGTCGGCC GANAAAACTGAAGGAGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTAC AAGGACCAGGCCGACAAGGCATCTACCCGCCTGAAGCAGCTCAAGCGGCAGCTGGAGGAG GCTCGAAGAGGAGGCCCAGCGGGCCAACGCCTCCCGCCGGAAACTGCAGCGCGAGCTGGA GGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCTCCCTAAAGAACAAGCT CAGGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCGCCGGGGA TGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATA AGCCTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCC AGACCCCGCAGCACGCCTCTCCCCACCTTCTTGGGACTGCTGTGAACATGCCTCCTCCTG CCCTCCGCCCCGTCCCCCCATCCCGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTC CTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTGCTCAGAATCTGATACCAAAGAGACATG GCCCGGGCCCAGGCAGAGAGCCACCAGCAGGCTCCTCAGCCCTCTCTTGCCAAAAGCACA AGATGTTGAGGCGAGCAGGGCAGGCCCCCGGNGAGGGGCTCAGAGNTTTCTATGAATCTA TGTTTCTTCAGACTGAGGCCTNTTTGGTAGTCGNAGCCCCCGCAGTCGTCAGCCTCCCTG ACGTATGGCAGCAGNGCCCNCACTCTCCTNCTTTTTTTGCTGATCCAACACCACGTGGGG ACTNACACACCTCTGCCCATGGGCCTNCCACTCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK025393 unedited GGTCCTCTATGNACGCGGCCGCCTTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTAGCACATGGGTTCTCTTTATTGGGATGCTCAGTGGAAAACATCTGTAAACCAG CTTACTGTAGTGGTGACAGCTACAACACATGCTAAGGCACTTTTATTATATAAAAAAGGG GGTAGGGTGGGAGTTTCACTCACTCCTTTGTGACAGCAACTGGGACCTGAATTCAAAGCT TTGTAGGCATATGCAGCTTTTATATCACTTCATGATTTGAGAGTTTGTTTCCTTTAAAAA AAAAAAATGCCTTCTTGCCGTAAGTCTCAATGCAGCATATACAAAACAGTTAGGAATACA AGTAAATTCGGCAACCAGTGTAGACCAGTGAGGACGAGCTACTCTCTTCTAAACAAAGGC AGTGAGAAAGACTCGGAATTTTATCATTTATTACAAGAAAGGGGAAAAAAGAAAACCTTA TTTTGCTTTCAAAAATACCTGTGCAGGTGGCAGATTTTTAAACTATACTGAGTCAACGGT GCAGGTAAACCACCCTCGGGCCCAGTCCTAGAGTAGACACAAGATCGCCTGGGAGGGCCG CTGGCCCCTCTAACGCTCTGGCTGTCAGACTTGGGACAAGTCCCTTAACCATTAAATATA TTTTGTCCCCAAGAGTGTTGGGAGAAGCCACTGGGAGGCTGCTGGCCTTTCCAGCTTGAC CCCAACANCCTTGCTGTGGCAAAAGCAGGCTGTCCCGGGTAAAAGGAAGGGAGGGGGCAT GCACTTTGAAACTTTTTAGAATCCAGAGGGAGACCCCGGACAGTGGCGCTGCCTGGGACA GGGGGGGGAAGGAATCCATCCTCCCCCACCCCGACTTTTT
Restriction Sites	NotI-NotI
ACCN	AK025393
Insert Size	1850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK025393.1
RefSeq Size	1968 bp
RefSeq ORF	1968 bp
Locus ID	4627
Protein Families	Druggable Genome
Protein Pathways	Regulation of actin cytoskeleton, Tight junction, Viral myocarditis
Gene Summary	This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

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