PSG2 (AK002132) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CEA; PSBG2; PSG1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK002132, the custom clone sequence may differ by one or more nucleotides GCAGAAGGAGGAAGGACAGCACAGCTGACAGCCGTGCTCAGGAAGTTTCTGGATCCTAGGCTCATCTCCA CAGAGGAGAACACACAGGCAGCAGAGACCATGGGGCCCCTCTCAGCCCCTCCCTGCACAGAGCACATCAA ATGGAAGGGGCTCCTGCTCACAGCTACTCAGGAGGCTGCGGCAGGAGAATCACGTGAACCCAGGAGGCAG AAGTTGCAGCGTGCCGAGATAGCGCCACTGCATACCAGCCTGGGCGACAGTACGAGACTCCGTCTCAAAA AAAAAAAAAAAAAAAAAAAAAAAAGAGAAAAAAGAAATAAAAGGAAAGAAGGCTCTGTTGGAGCCTGGAT AGGGGAAAATATACCAGAGAGGGACAGGGGTCAAAACAGGAAAGTCACATTGAACTGGAATTGGTAAGAG GTAGGAAAATCTTAAGTGTTCTGTTTTCCTGATTAATCATCAGGGGCCACATTTTGAAAAATGATAATAA TAACTATATCAGATGACACTTCAAATAAAAATATAAGCAGGACGTGAAACACTGTCCTCAGCAAAAAACC TCAACAATTGGGGGAGAAAAAAAAAACACCAAGGGTGTGGAGGGCCCTGAGAAGTCTCACATCTACAGGA GTCTGCAGCCTGTTCCAGGCACTGGGGTGCAACCAAGATCACAAAAGTCCCTGTCCTCACTGAGCTCATG CTGTCATGGGGAGGAAGACAGAGGTCAAAACAGGAGAGGCAGAGTGGAGAGGGCAGAAAGACAATCTTTG AAGACCATCAGCCAACTGCCTTAGGAGGCTTAGGAGAGTCCATAGGAAGTCTAATGTCCCCAGAAGCAGA AACAGAAGACAGAAGATGTACCTGGTAGCAGCTTGTCCACAGGGATCTGATGTAACGGTGTTTTCTCATG GAAGCAAATTAATAATAAATGCTGTTTGTGTGAAACCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK002132 unedited NNNGGAGTCAGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGACA GCACAGCTGACAGCCGTGCTCAGGAAGTTTCTGGATCCTAGGCTCATCTCCACAGAGGAG AACACACAGGCAGCAGAGACCATGGGGCCCCTCTCAGCCCCTCCCTGCACAGAGCACATC AAATGGAAGGGGCTCCTGGTCACAGCATCACTTTTAAACTTCTGGAACCTGCCCACCACT GCCCAAGTCACGATTGAAGCCCAGCCACCAAAAGTTTCCGAGGGGAAGGATGTTCTTCTA CTTGTCCACAATTTGCCCCAGAATCTTACTGGCTACATCTGGTACAAAGGGCAAATCAGG GACCTCTACCATTACATTACATCATATGTAGTAGACGGTCAAATAATTATATATGGGCCT GCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGATCCAGAATGTCACC CGGGAGGACGCANGATCCTACACCTTACACATCATANAGCGAGGTGATGGGACTAGAGGA GTAACTGGATATTTCACCTTCACCTTATACCTGGAGACTCCCAGCCCTCCATCTCCAGCA GCAACTTANACCCCAGGGAGGCCATGGGAACTGTGATCTTAACCTGTGATCCTGAGACTC CGGACACAAGCTACCAGTGGTGGATGAATGGTCAGAGCCTTCCTATGACTCATAGGTTTC AGCTGTCCGGAACCCACGGACCCTCCTTCTATTGGTGTCCCAAAGTTACTGCAAGACCCT ATGATGTTAAATCGGAACTCAGGGATGGCCACCCGCATGAACCAGTCCCCTGAATCTCCT CCTGGTCCCAAACTCCCAGAATTCACCCTCAAACCCCATTAACGC
Restriction Sites	NotI-NotI
ACCN	AK002132
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK002132.1
RefSeq Size	949 bp
RefSeq ORF	949 bp
Locus ID	5670
Protein Families	Secreted Protein
Gene Summary	The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily of genes. Members of the CEA family consist of a single N domain, with structural similarity to the immunoglobulin variable domains, followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif, which has been shown to function as an adhesion recognition signal for several integrins, in the N-terminal domain (summary by Teglund et al., 1994 [PubMed 7851896]). For additional general information about the PSG gene family, see PSG1 (MIM 176390).[supplied by OMIM, Oct 2009]

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