KCNE3 (BC032235) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HOKPP; HYPP; MiRP2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032235, the custom clone sequence may differ by one or more nucleotides CCAGCTCCGAAGTCAATACTGAGATCCCAGATGTGTCCAGAGACATCCTGAAGAGGCTCGGGGGTGGAGG AGCCTTAGTGTGTCCACAAAGGGACTCCTGAAACTGACTGAGAGCCAGTGGATTTGCCAGCAGTCTGAGC TTCTACCGAGTCTTCCCCCACCTCAATCCCTGTTGCTATGGAGACTACCAATGGAACGGAGACCTGGTAT GAGAGCCTGCATGCCGTGCTGAAGGCTCTAAATGCCACTCTTCACAGCAATTTGCTCTGCCGGCCAGGGC CAGGGCTGGGGCCAGACAACCAGACTGAAGAGAGGCGGGCCAGCCTACCTGGCCGTGATGACAACTCCTA CATGTACATTCTCTTTGTCATGTTTCTATTTGCTGTAACTGTGGGCAGCCTCATCCTGGGATACACCCGC TCCCGCAAAGTGGACAAGCGTAGTGACCCCTATCATGTGTATATCAAGAACCGTGTGTCTATGATCTAAC ACGAGAGGGCTGGGACGGTGGAAGACCAAGACACCTGGGGATTGCGTCTGGGGCCTCCAGAACTCTGCTG TGGACTGCATCAGGTCTCAGTGTCCCTATCTGTAAGATCAACAAGAAACACGGTTAAGGGAGGTCGTCAC TGGGGTGGGAGAAGAGGGGCTGGTAGACCGAAGCCTTGTGCATAAGGATTTTTTCCCAGGAAAAGATAGA CTTTATAAACAGTGGGAGCCCATGAACAAACATATAAAAGTAGCAACAGATAATGACCAATAACTGGTTC AGTGGCTGGAGTATTAGGGGCCTGGGGATTGGAGAACGGAGAAGAAGTTGTAGCAGAGGGAAATGAGACA GGAAGATGCTCTGGGGACACATTTTTTATGTGTTATCTTCAGCCATGAGAAGCAGTGATGACTATCCCAT ATCACAGATATGATTTACCACCACCACCCTGCCCCCGCTCCCGTGAAGAAAGCAGGGCAAGTGCTGTGCT GCCCATTTGGGCCTGCATAGTGCCATGATTGGAACCCAGGAACTCTGGTCTCCTTGCCTAGTGCTTTTCA AAACTCTGTGCTACACAGGAGTGGATCCAGGCCTGAAGGTCATACAATTCTGGGGACTCTCTTTAAGAAA AAGAATTCTAAAATATCTTACTTTTGCAAACATTATGAAAATATACTGCCACATTAATATGTTGCTAGGG CCCCTGCTAGGACCTTAAGAAGGAGCTCATGTGAGTCAGGACCCTGAATGTTAGGCCTCGTTAGCTCTAT GGTTCATATGCTTCTTGAACCAAGTCACAGGGCACTTCCCAGCCACATTGCCAGGCAACAGGACTAAACT ACCTCCAAAGCAAGCAGTCTTTTCAGTTTTGACTGAGTGATGTGAGAAACTTCTTTTCTTTTCTTTTCTT TTTTTTTTTTGAGACAGTCTCCCTATGTCACCCAGGCTGTGGTGCAGCAACCCAATCTTGGCTCGCTGCA ACCCCCACCTCCCGGGTTCAAGCAATTATCCTGCCTCAGCCACCTGAGTGGCTGGGATTACAGGTTCCTG TCACCACACCCAGTTAATTTATATATATATATATATATATATATATATATATATATATTTAAGTAGAGAC AGGGTTTCACATGTTGCCCAGGCTGGTCTCGAACTCCTGTCCTCAAGTTATCTGCCCATTTTGGTCTCCC AAAGTGCTGGGATTACAAGTGTAAGCCACCACGACTATCTGAGAGAAGTTTTCTGATGTCATGTTGAATC TGCTTCTAAAAGACTGATACTGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGC CAACATGGTGAAACCCCATCTACTAAAAAAATACAAAAATTAGCCAGACCTGGTGGCGGGTGCCCGTATT CCCAGCTACTTGGGAGGCTGAGGCGGGAGAATTGTTTGGGCCCGGGAGGTGGAGGTTGCAGTGAGCCAGG ATCACGCCACTGCACTCCAGCCTGGGTGACAGAGCAAGGCTCTGTCTCAAAAAAAAAACAAAAACAAAAA CAAAAAAGACTGATATCGCACCTAAATTATTATTATATTAAAAGAAGCAGAGTATGAGAGACAGGTACAT GGTCCAGTAGGAAGAGAAGCAGCCCTGATTCTACCACTTAAGGTGATGTATGATCTTAGGCTGGACACTT CTCTCCCTCATCCGTTTTCCTCTTCAACATAATGAAATAGACTTGAAAGTCTCTAAGGCTCTATCAGTTC TGACATTCTAGGCTTCATATACATTAAGTTGAGCCATATGTAATCACTGTGTTTGTAGGTTAGAAACAGC TGAGTATCGTAGTTTCATATATGGTTCCAGCTAATACATGCAATGTGGCTGGTGAACACTTCTGAATTCA GAAACTATCCCAGATCTCAGCTAGAACCATCCACTGTTCTGTTTGTCCAGTTTCAACTTAAGGGATCTCC ATGCGGTCCCTGGAAGTACCCATTGAAACATGCGTATTTGTGTATAGCAGAACTCTGAAATAATATTCTG ACAGCAGTTATCTCTGAGGAATTGGGTTATAGGTGATTTTCCCTTTCCGCATGATAAATTTATGTAATAT TTGACTGACTTGACCGTAAGTATGTTACTTGTATAATAAAAGAAAAAAAGGTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032235 unedited CAACATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCTGGGCTTT CCCAGCTCCGAAGTCAATACTGAGATCCCAGATGTGTCCAGAGACATCCTGAAGAGGCTC GGGGGTGGAGGAGCCTTAGTGTGTCCACAAAGGGACTCCTGAAACTGACTGAGAGCCAGT GGATTTGCCAGCAGTCTGAGCTTCTACCGAGTCTTCCCCCACCTCAATCCCTGTTGCTAT GGAGACTACCAATGGAACGGAGACCTGGTATGAGAGCCTGCATGCCGTGCTGAAGGCTCT AAATGCCACTCTTCACAGCAATTTGCTCTGCCGGCCAGGGCCAGGGCTGGGGCCAGACAA CCAGACTGAAGAGAGGCGGGCCAGCCTACCTGGCCGTGATGACAACTCCTACATGTACAT TCTCTTTGTCATGTTTCTATTTGCTGTAACTGTGGGCAGCCTCATCCTGGGATACACCCG CTCCCGCAAAGTGGACAAGCGTAGTGACCCCTATCATGTGTATATCAAGAACCGTGTGTC TATGATCTAACACGAGAGGGCTGGGACGGTGGAAGACCAAGACACCTGGGGATTGCGTCT GGGGCCTCCAGAACTCTGCTGTGGACTGCATCAGGTCTCAGTGTCCCTATCTGTAAGATC AACAAGAAACACGGTTAAGGGAGGTCGTCACTGGGGTGGGAGAAGAGGGGCTGGTAGACC GAAGCCTTGTGCATAAGGATTTTTTTCCCAGGAAAAGATAGACTTTATAAACAGTGGGAG CCCATGAACAAACATATAAAAGTAGCAACAGATAATGACCAAATACTGGTTCAGTGGCTG GAGTATTAGGGGCCTGGGGATTGGAGAAACGAAAGNAAGTGTAGCANAGGAAATGANACA GGAAGAGCTCTGGGGCACATTTTTTATGTGTATCTCAGCCTGAGAAGCATGATGACTATC CATAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC032235 unedited NGCCCCTCTAGNACGCGGCCGCCTTCTAAGATCGGTTTTTTTTTTTTTTTTTTATTTTTT CAAAATAGAATACCTTTTTTCCTTTTATTATACAAGTAACATACTTACGGTCAAGTCAGT CAAATATTACATAAATTTATCATGCGGAAAGGGAAAATCACCTATAACCCAATTCCTCAG AGATAACTGCTATCAGAATATTATTTCAGAGTTCTGCTATACACAAATACGCAGGTTTCA ATGGGTACTTCCAGGGACCGCATGGAGATCCCTTAAGTTGAAACTGGACAAACAGAACAG TGGATGGTTCTAGCTGAGATCTGGGATAGTTTCTGAATTCAGAAGTGTTCACCAGCCACA TTGCATGTATTAGCTGGAACCATATATGAAACTACGATACTCAGCTGTTTCTAACCTACA AACACAGTGATTACATATGGCTCAACTTAATGTATATGAAGCCTAGAATGTCAGAACTGA TAGAGCCTTAGAGACTTTCAAGTCTATTTCATTATGTTGAAGAGGAAAACGGATGAGGGA GAGAAGTGTCCAGCCTAAGATCATACATCACCTTAAGTGGTAGAATCAGGGCTGCTTCTC TTCCTACTGGACCATGTACCTGTCTCTCATACTCTGCTTCTTTTAATATAATAATAATTT AAGTGCGATATCAGTCTTTTTTGTTTTTGGTTTTGTTTTTCTTTGAGACAGAGCCTTGCT CTGTCACCAGCCTGGGAGTGCANTGGCGTGATCCCGGCCCACTGCAACCTCCGCCTCCNA GGCCCAAACAATCCTCCCGCCTCAGCCTCCCAGCAGCTGGGAATACGGGCACCCGCCACC AGGTCTGGCTATTTTTGTATTTTT
Restriction Sites	NotI-NotI
ACCN	BC032235
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032235.1
RefSeq Size	2684 bp
RefSeq ORF	2684 bp
Locus ID	10008
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
Gene Summary	Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to modulate the gating kinetics and enhance stability of the multimeric complex. This gene is prominently expressed in the kidney. A missense mutation in this gene is associated with hypokalemic periodic paralysis. [provided by RefSeq, Jul 2008]

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