Cytohesin 3 (CYTH3) (AK023609) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARNO3; GRP1; PSCD3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023609, the custom clone sequence may differ by one or more nucleotides TACTTTCTAACAGTAGTATTTTTAGAATGGCAGCTATAAAGTTAACTCCTGGACACAAGTATATACTGTG CACTGAAAAAATATCCATCTACACAGCACCCAAGGGGAGGGCTGGGGGCACCGGCACGGGGGCAGCGTGC AGCCCTGCCCTGTCAGGCTGTCAGACAAGCCCCGGGGGGCAGCAGGTGGGCTCGGGACGGGCTGGGGGAG GGACGGCCATGGCACTTGGGGGCTCCAGGGTGACTCCCATGAGGCCTCCCTTCAACCAGGCTTTTTGGCC CCACAAATACTTTAAGCAAATCATTAAAATTATAACAGTTAATGGTTTGGGGGTGTTTAGGCTGTAACTG CTAACTCCTAGGAAACAGCCTTTTCCCTGGACACAGATGGTCCATACGCTGAGCCACGTGAAACTGCTGA TGTTTTGTTTAGATGCACACACATGGCAGCGTTTCATACAGGTCAGCAGGTTAGACCGGCTTTTGACCAT ATTCATCGCTATTTAAAACCTGTGGCAAAATGAACGCTTATTTTACAGACTTTCTAATTTGACCAGATTT CTTAATGAATAGACACAGAATTAACTAAAAACAGTCTCACCCCATGTAGTGCGCCGTGTCCTGAGAGAGG TGCCCTCCCTACGAGGAGGGAAGAACAGGCCCTGGGGTGCAGAGGCCCGGCACGTAGAGAACCCAGATAG ACGCCGGTGGTGGAACTGGTCAAACTCCACGCCCGCCTGGGAGGTTGTCAGGTTGCTGTGGATGTAAGGA TAGGAGGTGCCCAGTGCTCCGCTCAAGGAAGGCTGGATCTGGGCCCCACCTACAGAGAGGGCTCAGGGCT GGACCGGGGGCATTGTGTGCTTGGGCCGACCCGGGCCGGTGGCAGACGCTGTTCTCTGTCGGGAGATTTG CGTCCCCAGGACCCTGTTACACAGTGGGCTGTTGGGTTGGTGGCTGGCTTTTCCTCTATGGACTTCCTCT TCCTGCCCCACCTGCATAGGCACACACACCTTGAATCTGCACCCTCTGGAGGGCATCTGTACTCCTGTGC AAAATGCCCAGTCCAGAGACAAAACCTCAGACTTTGTGCACCTAGGTTTCCTTCTCAGCAGCGGAGACTG TTCTTTGAGTTGCCTTGAAGTGGAGGCCGAGCGGCTGCGGGCCCTTCGCCTCCCTGCGGCTGACCTTGAT GTAGCTTTAAGTCACACTAGACTGCAGAGGGGTCCGAGGCCAGAAACCCCTGTCCTGCATCAGACTTTCA TTCCCACGTTCTTAGGCTTTGTTACTGATACCTCAAATCGGAAGTTTTAGTTCTGAGAAAGGCAAGTCAG CGCTCTTGAAATGCCTGACTGGTAGATATGCAACTCTGGCCTCCAGTCTTCCATGAAAATAAATGCTGCC TGGACCCCCACCCAGACCACACACTGACGGCCGGCTCCGGCGGTGCCCACCCCTCAGGCTGGCCCGGCAC CCAAGACTGGCCACAGCCAGCTCTGTCAGCATGTTGTGCTCGGACAAGCTGTTTCCTTCTTCTGACCAAC CCAGGTGTGACCTGGGGATGCAGAGCTTTCTGTTTTGGGTGTTGGGAGGAGCAGCAGGAAGGAGTCGCCA GATGATCAAGCTCCCCCTTTGCTGTCATCTGTGAATGAGCTTCGCCAGGTGGTGGGCACCTGGGAGCCAT GCAGAGGCTGTGGTGCTGAGTTAGACTCCAGGTACTTTGTGGTCAAAGGAAATCGCCTAGCTCCAGGCTG TGTTAGGACAGTATTAGCATGAAGGCTGTGCGACCATCATGCCTGCTGATCCTTGAGGCAGGCCTGGTCC AGAAAACTCTGGGTCAGTGACTGCGCAGGGCCAGCCGCTACCAGGACGGCCCTGAAACAGGACACATCTG TTTTTTGTCCCTCACCCTGGGCAGGCCGCGTCACAATCACAGTCCTCCTCCTCCCCACCCTGACGTCTGA GCGCAGGGCTTGAATTGTTAGTCCCAACTCTGGCCAAAGATACTTTTTCCAGAGACAGAGGCCAGGAGGC AGTGAGGGGAGCCCCGCGGGGAGGCGGCGGCGACTGCCACAGCCCTTCCAGCCTGTCTTGCTGGCCGCCC TGGTTCATATTTGAGTTTAATTGTACTGACCCTGGACCCAGATAAGCAGCAACTTTGTGTCTTTGGGGTC ACAGAACATTTTGGGGCAGTTTAATGTGGTACCAAACTGAAAATAGGAGCTATTTATAGATGGAGCAGCA CTTAGTGCTTCATAGAAAGCAATGCCTATTTTTAAAGTTACAAACGCAGATATCTACATAGATATGCTTT GCTGAGAAGTTAGGTCTGTGGTAGACCAGAAACCACAAATTGACTTTTTTTCTTAGAAAATATTTCTATT TGCGGTAAATATAGTAATATGTAAATAATGTACATCTGTTGATTTCTGGAGTGTCTGTTATTCAATGATG TATATACTCCCACAGCTCGCATGAAGGAACAGCCTCTATTGATACTTGGTTGTAAAGTGAAGTAAGATTG GAGGGTGGATGGCTGTCAGAGCTCTTGCAGATACTGTGTTCACTAAATAAAAATCACATGTATTGTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023609 unedited AAAGGTGCAGGATTTGTAACACGATTCACTTNAGGGCGGCACGCGAATTCGCACCAGNAA AGCCACTGTTTTNATAGCAAAACAGGNAAAGGAAAAGCTACCAGTTTTTTATTCAGAATT TTTCTCAGATATATAGGATTATAGCTTTTATATGCCTTTTTATATTCTGAAATTATAACA AAAGATACTTTCTAACAGTAGTATTTTTAGAATGGCAGTTATAAAGTTAACTCCTGGACA CAAGTATATACTGGGTGCACTGAAAAAATATCCATCTACACAGCACCCAAGGGGAGGGCT GGGGGCACCGGCACGGGGGCAGCGTGCAGCCCTGCCCTGTCAGGCTGTCAGACAAGCCCC GGGGGGCAGCAGGTGGGCTCGGGACGGGCTCGGGGAGGGACGGCCATGGCACTTGGGGGC TCCAGGGTGACTCCCATGAGGCCTCCCTTCAACCAGGCTTTTTGGCCCCACAAATACTTT AAGCAAATCATTAAAATTATAACAGTTAATGGTTTGGGGGTGTTTAGGCTGTAACTGCTA ACTCCTAGGAAACAGCCTTTTCCCTGGACACAGATGGTCCATACGCTGAGCCACGTGAAA CTGCTGATGTTTTGTTTAGATGCACACACATGGCAGCGTTTCATACAGGTCAGCAGGTTA GACCGGCTTTTGACCATATTCATCGCTATTTAAAACCTGTGGCAAAATGAACGCTTATTT TACAGACTTTCTAATTTGACCAGATTTCTTAATGAATAGACACAGAATTAACTAANAACA GTCTCACCCCATGTAGTGCGCCCGTGTCCTGAGAGAGGTGCCCTCCCTACGAGGAGGGAA GAAACAGCCCTGGGGTGCAGAGGCCCGCACGTAGAGAACCCANTAGACGCCGGTGTGGAA CTGGTCAACTCCACGCCGCTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK023609 unedited AGTTAAATCCTTTTTTTTTTAACAATACATGTGATTTTTATTTAGTGAACACAGTATCTG CAAGATCTCTGACAGCCATCCACCCTCCAATCTTACTTCACTTTACAACCAAGTATCAAT AGAGGCTGTTCCTTCATGCGAGCTGTGGGAGTATATACATCATTGAATAACAGACACTCC AGAAATCAACAGATGTACATTATTTACATATTACTATATTTACCGCAAATAGAAATATTT TCTAAGAAAAAAAGTCAATTTGTGGTTTCTGGTCTACCACAGACCTAACTTCTCAGCAAA GCATATCTATGTAGATATCTGCGTTTGTAACTTTAAAAATAGGCATTGCTTTCTATGAAG CACTAAGTGCTGCTCCATCTATAAATAGCTCCTATTTTCAGTTTGGTACCACATTAAACT GCCCCAAAATGTTCTGTGACCCCAAAGACACAAAGTTGCTGCTTATCTGGGTCCAGGGTC AGTACAATTAAACTCAAATATGAACCAGGGCGGCCAGCAAGACAGGCTGGAAGGGCTGTG GCAGTCGCCGCCGCCTCCCCGCGGGGCTCCCCTCACTGCCTCCTGGCCTCTGTCTCTGGA AAAAAGTATCTTTGGCCAGAGTTGGGACTAACAATTCAAGCCCTGCGCTCAGACNTCAAG GTGGGGAGGAAGAGGACTGTGATTGTGACGCGGCCTGCCCAGGGTGAGGGACAAAAAAAG ATGTGTCCTGTTTCAGGNCCGTCCTGGTAGCGGCTGGCCCTGCGCAGTCACTGACCCAGA GTTTTCTGGGACCAGCCTGCCTCAGGGATCAACAGCATGATGGTCGCACAGCCTTC
Restriction Sites	NotI-NotI
ACCN	AK023609
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023609.1
RefSeq Size	2587 bp
RefSeq ORF	2587 bp
Locus ID	9265
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq, Jul 2008]

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