AKR1D1 (AK058142) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	3o5bred; CBAS2; SRD5B1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK058142, the custom clone sequence may differ by one or more nucleotides ATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGGTTATATCACAAGTCAA ATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCTGGCTTGGTGAAATCCCTGGGAGTGTC CAATTTTAACCGCAGGCAGCTGGAGCTCATCCTGAACAAGCCAGGACTCAAACACAAGCCAGTCAGCAAC CAGGTTGAGTGCCATCCGTATTTCACCCAGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCA TTACTGCATATAGCCCTTTGGGGACCAGTAGGAATCCAATCTGGAGGCAGCTCCATAAACTTGCCTTGCT TGATCTTCCCATACTACGAGTAACTCTCCATTCAGTGCATACTCTGCACTCAGAGAAACTGGTTACAGGT GACCTGCAGTGTCCTGGGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK058142 unedited NGGGTCAGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCANAATCACTCC TACAGTCAGGTTCTCCACAATGGATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGA TGGAAACAGCATTCCCATCATCGGACTTGGTACCTACTCAGAACCTAAATCGACCCCTAA GGGAGCCTGTGCAACATCGGTGAAGGTTGCTATTGACACAGGGTACCGACATATTGATGG GGCCTACATCTACCAAAATGAACACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGA AGGAAAGGTGCGGAGGGAAGATATCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGT CCCAGAGATGGTCCGCCCAACCCTGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGT GGATCTTTACATCATTGAAGTACCCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAG AGATGAGAATGGCAAATGGTTATATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGAT GGAAGCTTGCAAAGACGCTGGCTTGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAG GCAGCTGGAGCTCATCCTGAACAAGCCAGGACTCAAACACAAGCCAGTCAGCAACCCAGG TTGAGTGGCCATCCGTATTTCACCCAGCCAAAACTCTTGAAATTTTGCCAACAACATGAC ATTGTCATTACTGCATATAGCCCTTTGGGGACCAGTANGAATCCAATCTGGGTGAATGTT TCTTCTCACCTTTNGTAAGGATGCACTTCTAACTCATGNNGNAAAGGTCANNTAGAAGCA GCTCAATGTTTTGCGTTCACATCACGAGGGGNGGGGTGTCATTCTAAAAAGCTTATCTGA AAGAT
Restriction Sites	NotI-NotI
ACCN	AK058142
Insert Size	2560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK058142.1, BAB71682.1
RefSeq Size	1208 bp
RefSeq ORF	441 bp
Locus ID	6718
Domains	aldo_ket_red
Protein Families	Druggable Genome
Protein Pathways	Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways, Primary bile acid biosynthesis
Gene Summary	The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]

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