TOM1L2 (AK055959) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK055959, the custom clone sequence may differ by one or more nucleotides TCACCTTCTTGACCCTCTCTCCATCATTCAGCTGCCAGCCCAGGCTTCACACCCAAGCTGGCTCAGCAGC CGAGCCTGGCACCGAGGGTCCCTGCAGGCTCCCTGGGCAGGGAGAGGGCCAAGGACAATTGGGAGGGCAG CAGGCAGCCCGCAGATGGTGGCCATGTGGCACGCTGCTGAGACGACACTACCAATAAACCAAACTGCCAC GCACACACTGCAGGCTCACACCCGCACACCTGGTCCTGGCTTGTGGAGGGACTTGCACTGGAGGTATTGC TCTGGCTTGCCTGGGGAAGTGTCACAGATGTGCACATGGGCCACACTCAGTGCTGTGGCCTGGCCTGCCG TAGCCTCACACATCTGCCTCAAGAGCCAGCAGCAAACAGCTTTGTGGCATCCTTGGAGGTTCCCAGTGAC TGAGAGGCCTGGGCAGCACTAAGGCCCCTGCTCTGCTCCCTGGCATAGGCCTTGGCCTCCTTGCACAGAC TCTCCTGACAGTCTGGCCTCCTCCCACCTCAGAGGTCCTCTGGCCACCAATTTATACTCTCTTCCCAAGC TGATCCTGAAGGCAGGCAGAGCAGCTAGGGGCTGGTAACATGTATTCCCATCCCGTGTCCTCACTGGTGT GTCTCCTGGCCATGGGCAAGGGACTCGGGTCATCCCAGGCCCTGGTCCAGCCAGACACCTGGCCCCACAC CTCCCACCAGGCTGCTGTTGCTCAGAAGGAGAATACTGCAGCTGTATGCATCCCCGTGTGTCTTGGGCCC AGCTCGCTGGGTCAGTCTGCTCCTGTCCCCTCCAGCATGCACTGCCTAGGTCAGCACAGGTTCCTGATCC ATGGTGTAGTTAGCCCTTCCCACCTGGCAGGACGGCCCAGACCACCAGCCAAGCAGGGAAAGGGGGCTGG GCTTGACGGGAGAAGAGACCAGCGACATGGTCGTTGGGGAGCATTCAGGATGGCGACTTCAGCTGGGGGT CATGCTGAGCACCAACAGGAACTATTCCAGTGAAGAGCAAGTGCTGCCCGACCCAGGACCCTGTGCCAGG CTAGCAGCCCTCCAGCTCCCTCCAGAGAGGAAACCTCTGTCTGGCTGAGGGTGGGACTAGCTGGGATGTC TCACTCCAGTTGCTCAGGTTCACCCAGGAAGCTCCTCCGTGGAGTGGCCAGCCTGATTCTAGCCCTGTCC TCTCTGGCAGCACATGCCACACCTGCCTGGGCCTTCTGCTCCCTGATGCTTGATGAGCCCCTGCCTCCTC AATGTTTCTCAAAGACAGACCCCCCTGAGGCCAGCTTGAATGTGAAGACTGCTGAAGTCAGCTGGCTTCA CTTGAGCTGCAGAAAAGGTGGCTGGGATGGCCCAGGTGCACCCAGAGGCCCCAGCCCTTTGGCTGCCTTT GGGTTGTGACTTGGGTTGTCTCTGAGGCCCTGCCAGAGCTGGGCCTGCGGGTGGTGGGCGGTCCGACCTC GGGCAGTCAGTGCTCCGCAGCCTCAGCACTGCATCCCAGACCCAGTGTCCTCAGAGGGAAGAGCCAGCCT CCCTGCCTCATGGAACCAGGAGTCCCAAAAAGTCAGGAGCCTGGAGGCTCTGAAAGGAGCAGGGATTCCA TAGTGCGTGAAGCTGAAATAGGCGCCCTCCTGGGGAGCCCCCAGCAAAACTGTTTTTCATACCCACTCCC AGAACTGCCCCGCTCCAGCTCCAGCGCCAGCGCCAGCTGGTTGCCAGGCGTCATTGGAGAGGCCTGGCTG CCCCAGGGGCAGCAGGGAGTGGTGGACCTGTATGGGCTGGCAGGAGGCCATTGGCCATGCTGACAAGTGT CACCTGCCTTCCTAGCCTGGAGCCACCCCTCAGGTGGCCTGCTTGCACCTCCTATCCGGAGGTAGCCTGC CCCACCTGTAGGCAGAGGGGGCTCTTGCTTGAGGCCTGCACAGGAAGCAAGTATAGCCCCGGTGCCCCAG AGTGGGTTCCACTTAGCCCTGGCGAGATGGCCTGTCCTGAGATCTCTGCTCCCAGACCCCACCATCTGGG GAGCACAGTCCTTAGGCTGCCTGGTCCAGGAAGGGGGTGCGGCTCTGTCAGGAAACCTGGACTCTCAAGG CCCACCAGCCTCTCCGTGAGTGTTAGAAATCACAGATACAGTATATACTTAATTACACTAAATTATTGCT GGGATTCCTTATAAGCACTAATTATACCTGATTATAGGTTAAAATATTTATTTTGTCAAAATATTTTCTT GGGAATGTGTTTAACCCTTTCTGCGTTCATTGTTGCTGAGATGTGAAAACTAACCATTCCCTCCTGCCTA CCTTTTTGGCCACTGGGCGGCAGAGAATGGCGCTATGTGCAGTTGGGCCCCCGGCACCATGGGCCTTTGG CCTGCCTGCTGCAGAGTAGCCCTGCCTGGGCAGTCTCCAGGCACTGAGCAGGCCATCTGTGGCCAGGCTG AGAGAATGACTGGCTCGCTTACCAGCGTGCATGGGACAAGGAGCTTTGGAGCCTCAAGGGGTTGTTGCTG GCCTGGGCTAGAGGGAAAGGTGACCATCCGTCTGTCCTCCTGTCTTTCTATTAGCGCCTCCATGTGAGTG ATGGTGCCTTGGTTCACTAGCCTTCCCCCACCACCCCACCATGCCACCTGGTGGTCTTGGGGCCTGTGCT GTCACTCCAGCCCCTGGGGAGGAGAGGACCCAGCCCGGAGAGTTGGGGCAAGGGCTCCACATGGCCCAAG GGCAACAGATGCTCGCAGGGCAGCTGCTGCCGATGCTCACGCTCCTGCCCCCCTCCTTCCCGCTGCCACA CCCCACCCTGGGCCCCCGCAGACACGCATCTCTAACTCAGTTGGGCCCAGCCTTCTGGATAGCTTGGGGT AGGCCATGGGCCCACCTGGGGCCAGGCCAGCCCCTGGGGCAGCTCTGGAAGAGCAGTGTGGAGGAGCACT TGCTTGCAGCCTGGCTTCAGCCTCTGGCACTGCTGGAGTGGTCCCTGGGAGCTTCTGCACTGTCGGCTGT GGGGACGTCTCACCCACTTGGGTTACAGTAGGCCTTCCCCACCCAGAGAGAAGTGTTTCCACCCCAGAGA CATTGCCTGTCAGCCCCTGAAGTGCTCGCCTCCCCCAGTGCCCGTCACCAGCCCTTCCTATCTGTGGGGT CCAAGTCAGGCTTCCCCTGCGGCCACCAGCCATAGGGAGCAGCCATCAGCCCCCGAGTCAGAACTGCTTC TGTCTGTCCATACCTCCAGGCTCTCCCGGAGAGGGGGACGGATATTTATTTCCTAAAGTTTGCACTTAAT TGTGAGGATTCTCAGGATTGTTGGGGGCTACTGAAAAGAGGAATGTGTTGAATGTCGCGTTTGCTGTCCA CTCGTCCTAGAAGTTTAGTGTTTTTGTCACTGTCATGTGTTTCTGTGGGCAGAGCTGGTTCTGGAGGGTG GGTCAGTGCACCCGAGGCTCAGAGCATCCATCCACCCCACTGGCCCTCCTTCCAGATACCCTCTCTCTAA TTGGGTTCTTGCATGTAAAATACTCCACAATAAATAAATAATTGAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK055959 unedited GGGATGTGTGTTAAAAATTTATTTCTNCCNNNNTTTCTTTACCCCGCCCGNTGNCGCAAA GGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGNACTCATTTAGGTGACACTAT AGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGATTGCTCTG GCTTGCCTGGGGAAGTGTCACAGATGTGCACATGGGCCACACTCAGTGCTGTGGCCTGGC CTGCCGTAGCCTCACACATCTGCCTCAAGAGCCAGCAGCAAACAGCTTTGTGGCATCCTT GGAGGTTCCCAGTGACTGAGAGGCCTGGGCAGCACTAAGGCCCCTGCTCTGCTCCCTGGC ATAGGCCTTGGCCTCCTTGCACAGACTCTCCTGACAGTCTGGCCTCCTCCCACCTCAGAG GTCCTCTGGCCACCAATTTATACTCTCTCCCCAAGCTGATCCTGAAGGCAGGCAGAGCAG CTAGGGGCTGGTAACATGTATTCCCATCCCGTGTCCTCACTGGTGTGTCTCCTGGCCCTG GGCAAGGGACTCGGGTCATCCCAGGCCCTGGTCCAGCCAGACACCTGGCCCCACACCTCC CACCAGGCTGCTGTTGCTCAGAAGGAGAATACTGCAGCTGTATGCATCCCCGTGTGTCTT GGGCCCAGCTCGCTGGGTCAGTCTGCTCCTGTCCCCTCCAGCATGCACTGCCTAGGTCAG CACAGGTTTCTGATCCATGGTGTAGTTAGCCCTTCCCACCTGGCAGGACGGCCCAGACCA CCAGCCAAGCAGGGAAAGGGGGCTGGGCTTGACGGGAGAAGAGACCAGCGACATGGTCGT TTGGGAGCATTCNAGATGGCGACTTCAGCTGGNGGTCATGCTGAGCACCACAGCAACTAT TCCAGTGAGAGCAGTGCTGCCCGACCCAGNACCCTGTGCCAGNCTAGCAGCCCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK055959 unedited AACTACTATGTANCGTCGGCCGCNCTTCTANNGATCGAGTTTTTTTTTTTTTTTTTTGTT CAATTATTTATTTATTGTGGAGTATTTTACATGCAAGAACCCAATTAGAGAGAGGGTATC TGGAAGGAGGGCCAGTGGGGTGGATGGATGCTCTGAGCCTCGGGTGCACTGACCCACCCT CCAGAACCAGCTCTGCCCACAGAAACACATGACAGTGACAAAAACACTAAACTTCTAGGA CGAGTGGACAGCAAACGCGACATTCAACACATTCCTCTTTTCAGTAGCCCCCAACAATCC TGAGAATCCTCACAATTAAGTGCAAACTTTAGGAAATAAATATCCGTCCCCCTCTCCGGG AGAGCCTGGAGGTATGGACAGACAGAAGCAGTTCTGACTCGGGGGCTGATGGCTGCTCCC TATGGCTGGTGGCCGCAGGGGAAGCCTGACTTGGACCCCACAGATAGGAAGGGCTGGTGA CGGGCACTGGGGGAGGCGAGCACTTCAGGGGCTGACAGACAATGTCTCTGGGGTGGAAAC ACTTCTCTCTGGGTGGGGAAGGCCTACTGTAACCCAAGTGGGTGAGACGTCCCCAAAGCC GACAGTGCAGAAGCTCCCAAGGACCACTCCAGCAGTGCCAGATGCTGAAGCCAGGCTGCA AGCAAGTGCTCCTCCACACTGCTCTTTCAGAGCTGCCCCAGGGCTGGCCTGGGCCCCAGT GGCCCATGGCCTACCCCAGCCATCCAGAATGCTGGGCCCACTGANTTANAAAGCGTGTCT GCGGGGGCCCAGGTGGGGTGTGGCANCCGGAAAGAAGGGGGCAAGATCTTGAACATCGCA GCAACTGCCCTGCGAGCATTTGTTGCCTTGGGCCATGTGGAACCCTTGCCCAA
Restriction Sites	NotI-NotI
ACCN	AK055959
Insert Size	3700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK055959.1
RefSeq Size	3547 bp
RefSeq ORF	3547 bp
Locus ID	146691
Gene Summary	This gene belongs to a small gene family whose members have an N-terminal VHS domain followed by a GAT domain; domains which typically participate in vesicular trafficking. The canonical protein encoded by this gene also has a C-terminal clathrin binding motif. This protein has been shown to interact with Tollip, clathrin and ubiquitin and is thought to play a role in endosomal sorting. This gene resides in the 3.7 Mb deletion of chromosome region 17p11.2 that is associated with Smith-Magenis syndrome. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Apr 2017]

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