Laminin alpha 4 (LAMA4) (AK027151) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMD1JJ; LAMA3; LAMA4*-1
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK027151, the custom clone sequence may differ by one or more nucleotides TTTTAAAGAGCTGAGCCAAAAAAGCAACAGACAAGGCAAGTGGGCATTCGACCACATGCCAGACTAAAGA ACTTCTCTTCCTCTTAAATATAACCCATAGATCAGTCCTTCAAATCCCAAAAGTAAGTACATGTCGCCCA TGCTGTGCATACAGGGTGGTGTCCTAGCCTTGAATCATTTCTGTGCTCTTAGATCACATTCTGTCCTTAA CCACGCACACAAAAACACACACATACACACAGACGCCCACACAAACCCTTTCTTCAGAGGCCACATTTGA GTTGGGCATTATTTTTGACAAATACTGTGTGAGATCTCTGAAACAGAATCTCCCAAGTTGAGAGAATGGA ATCAGCTTCTCGGAGAAGAGCTCCATTCACGCTGAGCCAAATCTGCTTGAGCAGTTATTTGTACACAAGT GCCATCAATGTTGATGGGAATTTGGAATGAAGCACTGAAAACAGCATGTTAGGCCAGTGAGAATAATCCT TGAAATCTCTGTGGTAATTCAATTTCAGCAGTGGTTGGACCACCCAGGAGCCACTGGAAGCATGGTGCAT TTCAAGGTCTGCCTATTTTTTCAACAATTTTCTCTTTGAAACTTTAAGCTCTTCAGTCAGCACAATGTCA AAATGATCAGCAGACATGGGCAATTTTCTAGTCCAGCAACTTGAGATACAGTAAAAATTCGAAGAATAAA TTACATATGAAAGGCAGTTGGTTGGGACTGAACTGTGACTGATGATATAGGAATAGCACCAGATTTTATA GCATTCTGTCATTCTAAAACTGGCCATCAAGTGAAGTAACTTAGGAATGGAAAACCAGACGTTGTATGTC CTAACTGATACGTGGGAGCTAAGCTATGAGGACGCAGAGGCATAAGAATGATACAATGGACTTTGGGGAC TTACAGGGAAGAGTGGAGTGGGGAGGGATAAAAGACTACAAATATGGTGCAGTGTATACTGCTCGGGTGA TGGGTGCACCAAAGTCTAACAAATCACCACTAAAGAACTTACTCATGTAACCAAATACCACCTATACCAC AATAACTTACGGAAAAATTAAAAAATTTTTTAAAAGTAAAACTGGCCATCACATTACCTTTCATGCATTA TTTTTGGGATGATTTCGTGTGTTATAGAACTTTCACATGCCAGGAAGATTTACGTTTAGGAAATACATTA TGCCCACCCACCCCTATCTGTGCTTATACAACATTCTTTTCTCATACATTTAATCTAGCCTTTATACGGA TTATTTGTAGGAAAATCAAGCCTCCAGAAAAGGACAACTTGTTCAGAAGGAAAGCATGGACACCATTAAC CACGCAAGTCAGCTGGTAGAGCAAGCCCATGATATGAGGGATAAAATCCAAGAGATCAACAACAAGATGC TCTATTATGGGGAAGAGCATGAACTTAGCCCCAAGGAAATCTCTGAGAAGCTGGTGTTGGCCCAGAAGAT GCTTGAAGAGATTAGAAGCCGTCAACCATTTTTCACCCAACGGGAGCTCGTGGATGAGGAGGCAGATGAG GCTTACGAACTACTGAGCCAGGCTGAGAGCTGGCAGCGGCTGCCAATGAGACCCGCACTCTGTTTCCTGT CGTCCTGGAGCAGCTGGATGACTACAATGCTAAGTTGTCAGATCTCCAGGAAGCACTTGACCAGGCCCTT AACCATGTCAGGGATGCCGAAGACATGAACAGGGCCACAGCAGCCAGGCAGCGGGACCATGAGGTACAGC GGACACTCACCATCAATGCATTTCAGCATCAGCAGTGTGATGGTACACCAAGAAGGGGCTGTTGATATAA ATTTTTTTAAAATATTGGTATAGTTAAATACTTATATTTTAAAATATTGGTGTGTTTTTTGGTGCTATAA ATTACTAACTTGTGTGTTCCTAAAATCAAGTTGAAACTAGGATAATTGTCTAGTTCTTGCTTTGATAAGA ACGCAGTAGTTCTGATGCTTGTGTCCATGTGTATGGGTCTGTTATTCTTGCAAGTGGGTAAGCAATGCAT AGCTTTTTTTATACTGAGAGCACTAGAAGGCCAAAGCATCTCAAAACCATGGGTTCTGGGTATGCATAAT TTTTGGAAAGGCACGATAAGCAAATCTCACAGTCTGGCTGGTCAGCAGCTGCAGGGATAAGGAGACTAAT TGCCAAGGCCATGCAAATGCAAAGAGGAAGGTGAGGAGGATTCCCAGATGTGATCATAGCTTGCAAAGAT GGTCATCTCATGGGGCCAGAATTCGTGCATGCCCACACCTACAAAGCTGAGAACTGGTAATGGTCATGTG TCCGCTTCAAGCAATGCATGTATTCACAGCCGCCAGCATCAAGAGGGGCCATCTTTTGATAGGATGACAC ATGCATGTTCAAACATATTTTAAAAGATTAGGGAGAAAAGAATACTGTGAACAAATAGAAACCAATTATG AGCAAATTCACGAATTCGGTTATGTACTATTGTGGCTGGCAAAAGCCACTTAAACTAGGCTCTCTAGCCT GATTTTCAGCCATCATTACCCCTCACTAAGAATCCTTGTAAAATTTCTTCCGTAAAAGTTAATTACACTC ATCACCACTGTTTGCTAATGGGAGCAAACCCCTAGCCAATATTTTTTAGAATAATATGGTTGGGATAACT TTTTTTTTTTTTTTGCTAAATGAATGCATGATTATCTTATACAATAAAAGTATAACACAAAAAAAAAAAA AAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK027151 unedited TCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT TCGGCACGAGGGCGCTCTCCATCGAGGAAGGCAAATCCGGGGTGCTGAGCGTATCCTCTG GGGCCGCCGCTCATAGGCACGTGAATGAAATCAACGCCACCATCTACCTCCTCAAAACAA AATTGTCAGAAAGAGAAAACCAATACGCCCTAAGAAAGATACAAATCAACAATGCTGAGA ACACGATGAAAAGCCTTCTGTCTGACGTAGAGGAATTAGTTGAAAAGGAAAATCAAGCCT CCAGAAAAGGACAACTTGTTCAGAAGGAAAGCATGGACACCATTAACCACGCAAGTCAGC TGGTAGAGCAAGCCCATGATATGAGGGATAAAATCCAAGAGATCAACAACAAGATGCTCT ATTATGGGGAAGAGCATGAACTTAGCCCCAAGGAAATCTCTGAGAAGCTGGTGTTGGCCC AGAAGATGCTTGAAGAGATTAGAAGCCGTCAACCATTTTTCACCCAACGGGAGCTCGTGG ATGAGGAGGCAGATGAGGCTTACGAACTACTGAGCCAGGCTGAGAGCTGGCAGCGGCTGC ACAATGAGACCCGCACTCTGTTTCCTGTCGTCCTGGAGCAGCTGGATGACTACAATGCTA AGTTGTCAGATCTCCAGGAAGCACTTGACCAGGCCCTTACCATGTCAGGGATGCCCGAGA CATGAACAGGGCCCCAGCAGCCAAGCAGCGGGACCCTGAGAAACAACAGNAAGAGTGAGG GAACCAATGGAGATGGTGACATGTCTCTGAGCCATTTGCGGACTCTCTGACACACCTCGT TTAACTCTTTCAGACCTGATGATTTAATAAGAAAGCCGTCGGGATTATTGCCAAATCAAT GGGCCACAGTGACTACCG
Restriction Sites	NotI-NotI
ACCN	AK027151
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK027151.1
RefSeq Size	2747 bp
RefSeq ORF	2747 bp
Locus ID	3910
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
Gene Summary	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

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