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FUK (BC032542) Human Untagged Clone
CAT＃: SC104328
FUK (untagged)-Homo sapiens, L-fucose kinase, clone MGC:45494 IMAGE:5534376, complete cds
CNY 7,552.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110046B12Rik; FLJ39408; fucokinase; L-fucose kinase; OTTHUMP00000082728 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC104328 sequence for BC032542 edited (data generated by NextGen Sequencing)
ATGGAGCAGCCGAAGGGAGTTGATTGGACAGTCATCATCCTGACCTGCCAGTACAAGGAC AGTGTCCAGGTCTTTCAGAGAGAACTGGAAGTGCGGCAGAAGCGGGAGCAGATCCCTGCT GGGACGCTGTTACTGGCCGTGGAGGACCCAGAGAAGCGTGTGGGCAGCGGAGGAGCCACC CTCAACGCCCTGCTGGTGGCTGCTGAACACCTGAGTGCCCGGGCAGGCTTCACTGTGGTC ACATCCGATGTCCTGCACTCGGCCTGGATCCTCATTCTGCACATGGGTCGAGACTTCCCC TTTGATGACTGTGGCAGGGCTTTCACCTGCCTCCCCGTGGAGAACCCCGAGGCCCCCGTG GAAGCCTTGGTCTGCAACCTGGACTGCCTGCTGGACATCATGACCTATCGGCTGGGCCCG GGCTCCCCGCCAGGCGTGTGGGTCTGCAGCACCGACATGCTGCTGTCTGTTCCTGCAAAT CCTGGTATCAGCTGGGACAGCTTCCGGGGAGCCAGAGTGATTGCCCTCCCAGGGAGCCCG GCCTACGCTCAGAATCATGGCGTCTACCTAACTGACCCCCAGGGCCTTGTTTTGGACATT TACTACCAGGGCACTGAGGCAGAGATTCAGCGGTGTGTCAGGCCTGATGGGCGGGTGCCA CTGGTCTCTGGGGTTGTCTTCTTCTCTGTGGAGACTGCCGAGCGCCTCCTAGCCACCCAC GTGAGCCCGCCCCTGGATGCCTGCACCTACCTAGGCTTGGACTCCGGAGCCCGGCCTGTC CAGCTGTCTCTGTTTTTTGACATTCTCCACTGCATGGCTGAGAACGTGACCAGGGAGGAC TTCCTGGTGGGGAGGCCCCCAGAGTTGGGGCAAGGCGATGCAGATGTAGCGGGTTATCTG CAGAGCGCCCGGGCCCAGCTGTGGAGGGAGCTTCGCGATCAGCCCCTTACCATGGCCTAT GTCTCCAGCGGCAGCTACAGCTACATGACCTCCTCAGCCAGTGAGTTCCTGCTCAGCCTC ACACTCCCCGGGGCTCCTGGGGCCCAGATTGTGCACTCCCAGGTGGAGGAGCAGCAGCTT CTGGCGGCCGGGAGCTCTGTGGTCAGCTGCCTGCTGGAGGGCCCTGTCCAGCTGGGTCCT GGGAGCGTCCTGCAGCACTGCCACCTGCAGGGCCCCATTCACATAGGCGCTGGCTGCTTG GTGACTGGCCTGGATACAGCCCACTCCAAGGCCCTGCATGGCCGGGAGCTGCGTGACCTT GTCCTGCAGGGACACCACACGCGGCTACACGGCTCCCCGGGCCACGCCTTCACCCTCGTT GGCCGTCTGGACAGCTGGGAGAGACAGGGGGCAGGCACATATCTCAACGTGCCCTGGAGT GAATTCTTCAAGAGGACAGGTGTTCGAGCCTGGGACCTGTGGGACCCCGAGACGCTGCCC GCAGAGTACTGCCTTCCCAGCGCCCGCCTCTTTCCTGTGCTCCACCCCTCGAGGGAGCTG GGACCCCAGGACCTGCTGTGGATGCTGGACCACCAGGAGGATGGGGGCGAGGCCCTGCGA GCCTGGCGGGCCTCCTGGCGCCTGTCCTGGGAGCAGCTGCAGCCGTGCCTGGATCGGGCT GCCACGCTGGCCTCTCGCCGGGACCTGTTCTTCCGNNAGGCCCTGCATAAGGCGCGGCAC GTGCTGGAGGCCCGGCAGGACCTCAGCCTGCGCCCGCTGATCTGGGCTGCTGTCCGCGAG GGCTGCCCCGGGCCCCTGCTGGCCACGCTGGACCAGGTTGCAGCTGGGGCAGGAGACCCT GGTGTGGCGGCACGGGCACTGGCCTGTGTGGCGGACGTCCTGGGCTGCATGGCAGAGGGC CGTGGGGGCTTGCGGAGCGGGCCAGCTGCCAACCCTGAGTGGATGCGGCCCTTCTCATAC CTGGAGTGTGGAGACCTGGCAGCGGGCGTGGAGGCGCTTGCCCAGGAGAGGGACAAGTGG CTAAGCAGGCCAGCCTTGCTGGTGCGAGCGGCCCGCCACTATGAGGGGGCTGGTCAGATC CTGATCCGCCAGGCTGTGATGTCAGCCCAGCACTTTGTCTCCACAGAGCAGGTGGAACTG CCGGGACCTGGGCAGTGGGTGGTGGCTGAGTGCCCGGCCCGTGTGGATTTCTCTGGGGGC TGGAGTGACACGCCACCCCTTGCCTATGAGCTTGGCGGGGCTGTGCTGGGCCTGGCTGTG CGAGTGGACGGCCGCCGGCCCATCGGAGCCAGGGCACGCCGCATCCCGGAGCCTGAGCTG TGGCTGGCGGTGGGGCCTCGGCAGGATGAGATGACTGTGAAGATAGTGTGCCGGTGCCTG GCTGACCTGCGGGACTACTGCCAGCCTCATGCCCCAGGGGCCCTGCTGAAGGCGGCCTTC ATCTGTGCAGGGATCGTGCATGTCCACTCGGAACTCCAGCTGAGTGAGCAGCTGCTCCGC ACCTTCGGGGGCGGCTTTGAGCTGCACACCTGGTCTGAGCTGCCCCACGGCTCTGGCCTG GGCACCAGCAGCATCCTGGCAGGCACTGCCCTGGCTGCCTTGCAGCGAGCCGCAGGCCGG GTGGTGGGCACGGAAGCCCTGATCCACGCAGTGCTGCACCTGGAGCAGGTGCTCACCACT GGAGGTGGCTGGCAGGACCAAGTAGGTGGCCTAATGCCTGGCATCAAGGTGGGGCGCTCC CGGGCTCAGCTGCCACTGAAGGTGGAGGTAGAAGAGGTCACGGTGCCTGAGGGCTTTGTC CAGAAGCTCAATGACCACCTGCTCTTGGTGTACACTGGCAAGACCCGCCTGGCTCGGAAC CTGCTGCAGGATGTGCTGAGGAGCTGGTATGCCCGACTTCCTGCTGTGGTGCAGAATGCC CACAGCCTGGTACGGCAAACTGAGGAGTGTGCTGAAGGCTTCCGCCAAGGAAGCCTGCCT CTGCTGGGCCAGTGCCTGACCTCGTACTGGGAGCAGAAGAAGCTCATGGCTCCAGGCTGT GAGCCCCTGACTGTGCGGCGTATGATGGATGTCCTGGCCCCCCACGTGCATGGCCAGAGC CTGGCTGGGGCAGGCGGTGGAGGCTTTCTCTATCTGTTGACCAAGGAGCCACAGCAAAAG GAGGCCTTGGAGGCGGTGCTGGCCAAGACCGAGGGCCTTGGGAATTACAGCATCCACCTG GTTGAAGTGGACACTCAGGGCCTGAGCCTGAAGCTGCTGGGGACCGAGGCCTCAACCTGT TGCCCTTTCCCATGA Clone variation with respect to BC032542.1 522 c=>t;1428 t=>c;1656 c=>n;1657 c=>n >OriGene 5' read for BC032542 unedited
TCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT CGGCACGAGGGCCAGAATGGAGCAGCCGAAGGGAGTTGATTGGACAGTCATCATCCTGAC CTGCCAGTACAAGGACAGTGTCCAGGTCTTTCAGAGAGAACTGGAAGTGCGGCAGAAGCG GGAGCAGATCCCTGCTGGGACGCTGTTACTGGCCGTGGAGGACCCAGAGAAGCGTGTGGG CAGCGGAGGAGCCACCCTCAACGCCCTGCTGGTGGCTGCTGAACACCTGAGTGCCCGGGC AGGCTTCACTGTGGTCACATCCGATGTCCTGCACTCGGCCTGGATCCTCATTCTGCACAT GGGTCGAGACTTCCCCTTTGATGACTGTGGCAGGGCTTTCACCTGCCTCCCCGTGGAGAA CCCCGAGGCCCNCGTGGAAGCCTTGGTCTGCAACCTGGACTGCCTGCTGGACATCATGAC CTATCNGCTGGGCCCGGGCTCCCCGCCAGGCGTGTGGGTCTGCAGCACCGACATGCTGCT GTCTGTTCCTGCAAATCCTGTATCAGCTTGGACAGCCTTCGGGGAGCCACGATGATTTGC CTTCCAGGGAGCCCGGCCTACGCTCAGAATCATGCNGNTACCCTACTGACCCCCAGGGNC CTTGTTTTTGGCAATTACTACCCAGGCACTGAGGCAGAATTTAACCGGGGTGTCAGGCCT GATGGGCGGGTGCCACTGCTTTTGGGGGGTGGCTTCTTTTTTGGGGGGACGGCCGGACGC CTCCTATCACCCCGTGAGGCCGCCCTGTAGCCTGCACTACCTAGGTTGGACTCTGGGCCC GCTGTCCCCGGTNTTTGTGTTTTTGCATTCTCCCTGATGCTGGAACCGACCAGGGGAGTT CTTGGGGGGGAGCN >OriGene 3' read for BC032542 unedited
TNCTANGATCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTCAAGGACAGAGGAATTTATTCCAAAATGCCAGTCCTGAAAACCCATTTCCCAC AGGCCCCAGGTCACTTGGCACCCCCCGTAGTTTTGCTAGTTAAGGCCTGGGATGGCAGTG AATCACTGGGTCACTGCTACCAACTTGGCAAGGGACCGCCACCCTAACTCCCACGCTAAA ATGAGGGTTGAGGGTAAGTCTACAACTTTAAGCCATAGGGAATGCCTCAAAGGGCCTTTT CAGGGTTGGCCTTGCTAAGGGACTCCCAGCCATATGCCAAGGGTGGCTGTCAACCCTTTC CAGGGCCCTTGGGGAATGAAAAGGCCACCCATAGGATTTGTAAAGGCCACGTGGGAAACT AAACTTGGGACCTTCCTATGTCCAGGAGGAACAAAGGCTACATTTAGGCCCCTGTCCACC AAGTCAAAGTCCTGTCCCAGGCACTTTTTGGTTGCCCAAATCTTGGCCTGGGCAACTTCC TTTGGGAGCAAATTCCCAAAGGTGGGTGGGGAGTAAGAGGGGGGGAGGTCCCCTGGGGGA AAGAAAGGGGGGGGAAACTGTACCCCCGGTTTTTCTCCTGGTGCCAAAAAGAAACCCCCT TTTTGGGGAAAGGGCCCCAGGGTGGAGGCCCTCGGCCCCCAAAAATTTTAGGGTCAGGCC CCGGAAGGTTCCCTCTCAAACCCGGGGGTGCGCTTGAAATTCCCCAAAGGCCCCTGTTCT TGGGCCCAAAACACCCCTCCAAGGGCCCCCTTTTTTGTGGGGCTCCCTTGGCAAAACAAA AAAGAAAACCCCCCCCCCCCGC |
| Restriction Sites | NotI-NotI |
| ACCN | BC032542 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC032542.1, AAH32542.2 |
| RefSeq Size | 3925 bp |
| RefSeq ORF | 3255 bp |
| Locus ID | 197258 |
| Domains | GHMP_kinases |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq, Jul 2008] |
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