ATP5H (BC032245) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP5JD; ATPQ; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase D chain, mitochondrial; My032 protein
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for BC032245 edited ATGGCTGGGCGAAAACTTGCTCTAAAAACCATTGACTGGGTAGCTTTTGCAGAGATCATA CCCCAGAACCAAAAGGCCATTGCTAGTTCCCTGAAATCCTGGAATGAGACCCTCACCTCC AGGTTGGCTGCTTTACCTGAGAATCCACCAGCTATCGACTGGGCTTACTACAAGGCCAAT GTGGCCAAGGCTGGCTTGGTGGATGACTTTGAGAAGAAGTTTAATGCGCTGAAGGTTCCC GTGCCAGAGGATAAATATACTGCCCAGGTGGATGCCGAAGAAAAAGAAGATGTGAAATCT TGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGGATTGTAGAATATGAAAAAGAGATGGAG AAGATGAAGAACTTAATTCCATTTGATCAGATGACCATTGAGGACTTGAATGAAGCTTTC CCAGAAACCAAATTAGACAAGAAAAAGTATCCCTATTGGCCTCACCAACCAATTGAGAAT TTATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032245 unedited GGGTTCAAAATTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACCGCCGGGT CGGTGAAGGATCCCAAAATGGCTGGGCGAAAACTTGCTCTAAAAACCATTGACTGGGTAG CTTTTGCAGAGATCATACCCCAGAACCAAAAGGCCATTGCTAGTTCCCTGAAATCCTGGA ATGAGACCCTCACCTCCAGGTTGGCTGCTTTACCTGAGAATCCACCAGCTATCGACTGGG CTTACTACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGAGAAGAAGTTTA ATGCGCTGAAGGTTCCCGTGCCAGAGGATAAATATACTGCCCAGGTGGATGCCGAAGAAA AAGAAGATGTGAAATCTTGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGGATTGTAGAAT ATGAAAAAGAGATGGAGAAGATGAAGAACTTAATTCCATTTGATCAGATGACCATTGAGG ACTTGAATGAAGCTTTCCCAGAAACCAAATTAGACAAGAAAAAGTATCCCTATTGGCCTC ACCAACCAATTGAGAATTTATAAAATTGAGTCCAGGAGGAAGCTCTGGCCCTTGTATTAC ACATTCTGGACATTAAAAATAATAATTATACAGTTCAAAAAAAAAAAAAAAAAAACTCGA CTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCCGGTTGCATTCCCTG TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTCCACTTCAGTGCCCACCAGCCTT GTCCTATAAAAATAAGTNGCATCATTTTGTCTGACTAAGTGTCCCTCTATATACTATCGG GTGAAGGGCGTGGATTGGACCAGGGGCAATTTGGAAAAACAACTTTTGGCCCGCGGGGTC ATTGGGAACCACC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC032245 unedited GGTACTACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGA ACTGTATAATTATTATTTTTAATGTCCAGAATGTGTAATACAAGGGCCAGAGCTTCCTCC TGGACTCAATTTTATAAATTCTCAATTGGTTGGTGAGGCCAATAGGGATACTTTTTCTTG TCTAATTTGGTTTCTGGGAAAGCTTCATTCAAGTCCTCAATGGTCATCTGATCAAATGGA ATTAAGTTCTTCATCTTCTCCATCTCTTTTTCATATTCTACAATCCTGGCCTTTGAGAGA GACACCCACTCAGCACAAGATTTCACATCTTCTTTTTCTTCGGCATCCACCTGGGCAGTA TATTTATCCTCTGGCACGGGAACCTTCAGCGCATTAAACTTCTTCTCAAAGTCATCCACC AAGCCAGCCTTGGCCACATTGGCCTTGTAGTAAGCCCAGTCGATAGCTGGTGGATTCTCA GGTAAAGCAGCCAACCTGGAGGTGAGGGTCTCATTCCAGGATTTCAGGGAACTAGCAATG GCCCTTTGGTTCTGGGGTATGATCTCTGCAAAAGCTCCCCCATCAATGGTTTTTAGAGCA AAGTTTTCCCCACCCCTTTGGGATCCTTCCCCGACCCTGGCTGTGGCCAAATTCTCGGGC CGCCCTTAGTGAGTCCTATTAAAAAAATTCTGACCGTTCATTAAACAAGCTTTGGTTATT TAAACCTCCACCGGTCCCCCCCTCCCGCCCTTTTTGGTTACCGGGGGGGGTTTTTCCAAA TTTTGGAAAGTCCCGTTGATTTTGGGGCCCAAAAAACCCCCTTTGACGTCAAGGGGGGGG AAACTGGAAATCCCCCTGGGTAAAACCCCTTTCCCCCCCCTTGTGGGTCTGCCAAAA
Restriction Sites	NotI-NotI
ACCN	BC032245
Insert Size	420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032245.1, AAH32245.1
RefSeq Size	592 bp
RefSeq ORF	414 bp
Locus ID	10476
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.