DTX3L (AK092957) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092957, the custom clone sequence may differ by one or more nucleotides ATCCATTGCATTTGCAGATATGCCTCAGGATTATTTTATTAGTACAATTTTTTTTTCTTTTTGAGACAGT CTCACTCTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCTCGGCACACTGCGGCCTCCACCTCCCGGGTT CAAGCGATTGTCCTGCCTTATTCCCTGGTGTATCTGGGATTACCGGTGTGCACCACCACACCCGGCTAAT ATTTTGTGTTTTTAGTGGAGATGGGGTTTCGCCATGTTGGCCGGGCTGGTCTCGAACTGCTGGTCTCAAG TGATCCACCTGCCTCAGCCTCCCAAAGTGGTGGGATTATAGGCATGAGCCACCGTGCCCAGCCTAGCGTC ATGTATTTATACTGTTATTTCCACCTCCTAAAAGTCAACAAGGACACTTAGCTCAAGAAGGCTGATGTTG AGAGAGAACGCTTATCAGAACAGGGATCTCTGAGGAAAAAGGGAGCTTAAATGTGTGTCCCTGGAATTTC TTCTAGCCCTTCCTTTCCTTGGTGACATGGCCACTGCTATGGGCGGGAATTAGAGAAGAGCCCTGACCAG TGGTGTTGATGTCTTTGCCAAGTATACTGTCAGGTCAGTGTCATAAATTGATGATATATATAGAGAGACT TCATTTAATAGAAAATAATATTTTAAGGACCACCTTGCTATTTTACTTCTCTAATAATGTTTTGAATATT TTGATAAATACCTTTAGCAACATAGTGGTTCTCATCTCAGCCCTCTCTGTCCTTGAAAGAATGTTATTTA TAACAGGGTGGTTTCTGGACTGCCCTTCTGGAGTAAAACTTGGGCATTCTAACCATATGCTTGCTGGAAA CCATAATAATGTAAAAAGTCGTCATTGGGTTTTCAATTGCAATTACATTGATATAATTTTGTATATAAAA CAGTCTGTTACTGGGAGCAGCATTGTTCATGTAAGAAAGATGTTCGCTTCATATAATACATGGAAACAAA GTTATATTTTCAATGTTAGCCATTACTGTGACACTCCAAAAAAGGCTGGTGATGGGGAGAAAGAAGAGCT TTAAAAAAACCTACAGACAATTCACTTGCCTCTCGGGCTTTTCTGCTTTGCCTGCCTTGACCGAATTAGA GTATCTGCATTTCTGCACTAAGCTGGTTGAGGTTTGCTAAGTCAATGGTAATGTTTTACAGAGCTTGGCT GTTAGCCAGCATTTGTGGAAAACGACCAATGTCTGTCATAGCTAGTGCTATAATTTATTCAGTTGGCATA TTGTTTATGGAGACAGTGAGATAAGGGGATTAGGATCCAGAGAGACTATTAAAATATGTGAGTCCATCTG TTGTGATCTGAGGTTTCATTAGAGAAAACCTGTCTGCTAGTACAACAAATGGAGACTTAGTAAGGATGTT TTTATATAAAGGATTATAAATCAGCAGAGGTAAATACATTAATCTTGGCCAAACAGGTCATGTCCTTTTG CGTTTTCCTGATGGCAGGTTCTGCCCCCTGCCGCCCGGGCACTGTTGGTCATAGCGTGACCTTGGCATCA GCTGTGAGCCGGTAAACATGGAAATGTAGGCCCCTCCCAACCTGAGTCAGACCCTTAGCCTGGGGTCCAG GAAACTGTTTTAACAAGCCCCTGGGTGATTTTTTTTCACATGTTCAAGTTTGAGCGCCTCGAGGGCCTGT GAATGGTTGCATATAAGGTACAGCTGGCCATATGTCTTAAATGACTTCAAAGGGGTGTGAGCCAGGCCTC TTCCCACACCAGACTTCATGAACCATGCCTGGTATTGTCATGTTTTTGTGAGCAGCCGTGAATAGGGCTG GGGGAGAGAGATGTTCAGCCAAGAAAGTCTAAAATAGAAAGGGAATGTTCAGTTATAACAAAACAAATTT TTGTAATTAGAGTGCTGGGTTGTGCTCAGCATCATTGGAGTTAAATGTGGAGCAGTGGCTTACACTTGTA ATCCCAGCACTTTGAGAAACTGAGGTGGGCGGATCCCTTGAGGTCAGGAGTTCGAGGCCACCCTGGGCAA CATGGTGAAACTCCATCTCTAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092957 unedited GGATTTTGTAATACGACTCACTATAGGGCGGCCGCNGATTCGGCACGAGGAGCCACCGTG CCCAGCCTAGCGTCATGTATTTATACTGTTATTTCCACCTCCTAAAAGTCAACAAGGACA CTTAGCTCAAGAAGGCTGATGTTGAGAGAGAACGCTTATCAGAACAGGGATCTCTGAGGA AAAAGGGAGCTTAAATGTGTGTCCCTGGAATTTCTTCTAGCCCTTCCTTTCCTTGGTGAC ATGGCCACTGCTATGGGCGGGAATTAGAGAAGAGCCCTGACCAGTGGTGTTGATGTCTTT GCCAAGTATACTGTCAGGTCAGTGTCATAAATTGATGATATATATAGAGAGACTTCATTT AATAGAAAATAATATTTTAAGGACCACCTTGCTATTTTACTTCTCTAATAATGTTTTGAA TATTTTGATAAATACCTTTAGCAACATAGTGGTTCTCATCTCAGCCCTCTCTGTCCTTGA AAGAATGTTATTTATAACAGGGTGGTTTCTGGACTGCCCTTCTGGAGTAAAACTTGGGCA TTCTAACCATATGCTTGCTGGAAACCATAATAATGTAAAAAGTCGTCATTGGGTTTTCAA TTGCAATTACATTGATATAATTTTGTATATAAAACAGTCTGTTACTGGGAGCAGCATTGT TCATGTAAGAAAGATGTTCGCTTCATATAATACATGGAAACAAAGTTATATTTTCAATGT TAGCCATTACTGTGACACTCCAAAAAAAGCTGGTGATGGGGAGAAAGAAGAGCTTTAAAA AACCTACAGACAATTCACTTGCCTCTCGGGCTTTTCTGCTTTGCCTGCCCTTGACCAATT AGAGTATCTGCATTTCTGCACTAAGCTGGTTGAAGTT
Restriction Sites	NotI-NotI
ACCN	AK092957
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092957.1
RefSeq Size	2052 bp
RefSeq ORF	2052 bp
Locus ID	151636
Protein Families	Druggable Genome
Protein Pathways	Notch signaling pathway
Gene Summary	DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]

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