JMJD1C (AK027280) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KDM3C; TRIP-8; TRIP8
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK027280, the custom clone sequence may differ by one or more nucleotides TTGCGGGGAGGGCCCGAGGTCGCTGTGTCCGGGGCAGAGCGGCCGGTTCGTCCCGAGTCTGCGCTCTTTC GGGTCCGCTGCTGTGTCCCGGTCCGCTCTCCTCAGCATGAGCGGCCGGTAGGAGTGAGGTTTCATCGGTT CCCTCGCACTGGAGGAGGCAGCGGCCGCTTCGGCAGCGACAGCTATGGCGGTAGAGACGCGGGCAGAGCT GGTGGGTAAGCGGTTCCTGTGTGTGGCGGTCGGCGACGAGGCACGTTCGGAGCGCTGGGAGAGCGGACGC GGCTGGCGAAGCTGGCGAGCGGGGGTCATCCGAGCCGTGTCACACAGGGACAGCCGCAATCCGGACCTGG CGGTGTATGTGGAATTTGATGATCTTGAATGGGATAAACGAGAGTGGGTTAAAGTTTATGAAGATTTTTC AACTTTCTTGGTGGAATACCACTTAATCTGGGCCAAAAGGAATGACCCTAGCCAGACTCAGGGATCAAAG AGCAAACAGATTCAGTGGCCTGCATTGACTTTCAAACCTCTGGTTGAAAGAAATATACCCAGTTCAGTCA CTGCAGTAGAATTCCTTGTAGATAAGCAACTGGATTTTTTAACTGAAGATAGTGCCTTTCAGCCCTACCA GGACGACATAGACAGCCTAAACCCAGTTCTCAGGGACAACCCGCAGCTTCATGAGGAAGTGAAAGTCTGG GTAAAGGAACAAAAGGTTCAGGAGATTTTTATGCAAGGTCCTTATTCCTTAAATGGATACAGAGTGAGAG TATATAGACAAGACTCTGCCACCCAGTGGTTTACTGGCATAATTACTCATCATGATCTCTTCACCCGCAC CATGATCGTTATGAATGATCAGGTACTAGAACCACAGAATGTCGATCCTTCTATGGTTCAAATGACCTTT CTAGATGATGTTGTTCACTCTTTGTTAAAAGGTGAAAATATTGGCATTACATCACGACGCAGGTCTCGTG CCAATCAAAACGTCAACGCTGTTCACAGCCATTATACACGTGCCCAAGCAAATAGTCCCAGACCAGCAAT GAACTCCCAAGCTGCTGTACCAAAACAGAATACACACCAGCAACAGCAACAAAGAAGTATCCGTCCGAAT AAGAGGAAGGGCTCAGATAGCAGTATACCAGATGAAGAGAAGATGAAGGAGGAAAAATATGATTATATAT CACGAGGAGAAAATCCTAAAGGTAAAAACAAACACTTGATGAATAAAAGAAGGAAACCTGAGGAGGATGA AAAGAAACTAAATATGAAAAGACTTCGAACTGACAATGTTTCAGACTTTTCTGAGAGCAGTGACTCAGAA AATTCAAATAAGAGAATAATAGATAATTCCTCAGAACAGAAGCCAGAGAATGAATTGAAAAATAAAAATA CTTCAAAAATAAATGGAGAAGAAGGAAAACCCCATAATAATGAGAAGGCAGGAGAAGAGACCCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK027280 unedited TGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCGGTGGCGGCGGCTGGGC TGCCTCGGGGTCTTCGACGGCCGCGTTGGGTTTTGTTTCAGTTTTCTTGTGTGGCGCTGC AGCACAAGGCTGCGAATCTGGGAGTCCAAAGGAGTGTTTGCGGGGAGGGCCCGAGGTCGC TGTGTCCGGGGCAGAGCGGCCGGTTCGTCCCGAGTCTGCGCTCTTTCGGGTCCGCTGCTG TGTCCCGGTCCGCTCTCCTCAGCATGAGCGGCCGGTAGGAGTGAGGTTTCATCGGTTCCC TCGCACTGGAGGAGGCAGCGGCCGCTTCGGCAGCGACAGCTATGGCGGTAGAGACGCGGG CAGAGCTGGTGGGTAAGCGGTTCCTGTGTGTGGCGGTCGGCGACGAGGCACGTTCGGAGC GCTGGGAGAGCGGACGCGGCTGGCGAAGCTGGCGAGCGGGGGTCATCCGAGCCGTGTCAC ACAGGGACAGCCGCAATCCGGACCTGGCGGTGTATGTGGAATTTGATGATCTTGAATGGG ATAAACGAGAGTGGGTTAAAGTTTATGAAGATTTTTCAACTTTCTTGGTGGAATACCACT TAATCTGGGCCAAAAGGAATGACCCTAGCCAGACTCAGGGATCAAAGAGCAAACAGATTC AGTGGCCTGCATTGACTNTCAAACCTCTGGTTGAAAGAAATATACCCAGTTCAGTCACTG CAGTAGAATTCCTTGTAGATAAGCAACTGGATTTTTTAACTGAAGATAGTGCCTTTCAGC CCTACCAGGTCCTTATTCCTTTAATGGATACAGAGTGAGAGTATATAGACCAGACTCTGC CACCCAGTGGTTTACTGGGCATANTACTCATCATGATCTCTTCACC
Restriction Sites	NotI-NotI
ACCN	AK027280
Insert Size	6000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK027280.1
RefSeq Size	1464 bp
RefSeq ORF	1464 bp
Locus ID	221037
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene interacts with thyroid hormone receptors and contains a jumonji domain. It is a candidate histone demethylase and is thought to be a coactivator for key transcription factors. It plays a role in the DNA-damage response pathway by demethylating the mediator of DNA damage checkpoint 1 (MDC1) protein, and is required for the survival of acute myeloid leukemia. Mutations in this gene are associated with Rett syndrome and intellectual disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

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