JNK1 (MAPK8) (AK094000) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK094000, the custom clone sequence may differ by one or more nucleotides TTTTTGTTTGTACATTTTTCTCAGAAGAGGGTTCACTACTTTTATACAATTCTCAAAAGATCCCATGACC CAAAGAAGATGATAAATAGTGTTGATGTGGCATCCACCATTAAGGTTAAGTGTGGTGTGCCCTGTGAGTC TGAATGTCTACTTAAGAACCTTAAGTAGACATTAAGAACCTTAAGAAGGTTTTTTGTTTGTTTTTGTTTT TTTGTTGTTGAGATGGAGCCTTGCTCCGTTGCCCAGGCTGGAGAGCAGTGGCGCAATCTCAGCTCACTGC AACCTCTGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGGCGCCT GCCCCCAAGCCCGGCTAATTTTTGTGTTTTTAGTAGAAATGGGGTTTCACCTTGTTGGTCAGGCTTGTCT CAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC CACACATGGCCGAAGGTTCTTCTTAAGTGGACATTCAGACTCACAAGGCACATCGCAGTTAACATCAGAA TCACTTCTGATGATAATATAAGTGAAGAATATAAGACAGGAGGCGCATATATTAATACCAGCAGAGCAGC TCCCAGTGTGTCTCTTCAGTTGGAACAGTTGTTGCAGTGGTCTACTTGCTGTCCAGAAGCCTGATAAGAG AAAAAGATTCCCATGGAGAAATGTTCTTCGAAGTGATAACCATGCTTACTCATAAGGAGTTGAAATGTAG CTTACCTGCTAGTTTTCCTCCAATAAAAATGTGTTTATCTTTCATTCTGATTTGTTGTGAAGCTTTTGCA CACTCTAATTTAAATCTTGGTAGCATATATCTAGTTGAGTACCCACAGTGCACCAGGCTCTATTCCAGGG CCCAGGAAATGGAAGTCAGTAAGACACGTGGTTCAAGCCCTCCCTGAGACAGATGGTAGTACAGAATGGT ATGTGGTATGATGTGCTGTAGCACAAGTTGCTGGTAGAGTGAAAAAGAAAGCTTCTACCCCAGCACTATC AAACCAACTTCAGAAACAGTGAATTACAGGAAAGATTAGTACTTCCTTTTAATATGATCCATTGTTGAGT GTCAAGGAATTGTTATTAATTAACATCCTTGAATCTTAGGCCGACATTTAACTGACTGTCATTGTAAGGA CACTGTTTGAAGTACTTCACATGTATAAAAATTTCCACTTAAACCATACATGCGTTGTGAGATTGGCTCT TAGACTTTGAAAAGTTCATTTTTGTTTACTTCTTTTACAGAATTGATATATAAGGAAGTTATGGACTTGG AGGAGAGAACCAAGAATGGAGTTATACGGGGGCAGCCCTCTCCTTTAGGTGCAGCAGTGATCAATGGCTC TCAGCATCCATCATCATCGTCGTCTGTCAATGATGTGTCTTCAATGTCAACAGATCCGACTTTGGCCTCT GATACAGACAGCAGTCTAGAAGCAGCAGCTGGGCCTCTGGGCTGCTGTAGATGACTACTTGGGCCATTGG GGGGTGGGAGGGATGGGGAGTCGGTTAGTCATTGATAGAACTACTTTGAAAACAATTCAGTGGTCTTATT TTTGGGTGATTTTTCAAAAAATGTAGAATTCATTTTGTAGTAAAGTAGTTTATTTTTTTTAATTTCAAGT GATGTAATTTAAAACCTAAGTTGTGTTTCAAAACAGCAACAAAACTGTATTGTATTTTTTTTGCTGTAAT TAACTGTATAATGTAAACCTAATTATTTTATCATGGTTTAAATTTTTTGCATATTTGCTTTATCTTATGC TGCTGATTTTTTTAACTGAATTTGTAAGATTTTGTTTATCAAAGCAACTATTATGTGGTAACTTGCCTAT ATCATGAATTATTTAAGATTTTTATAGTTTTTTTTAATTAGAATTTATTTCAGATGTTTTGTTCATGATA CTATCCTTCAGGGTTATGTGCTTATCAATGAAATAACCCCAGAGGAGTGAGGGAAAATAACTTGTAGCCA GTTATATTCAGGAATAACTACTGTAAATGATGAACGTGTTAGGAGACCTCCAATATTTGCTACTTGCCAA TCCTAATTTAGTTACAAGAATTGGTAGGCAATCCTACTTAATTTTGGCAAAAGCCCCGTCATCTAAATGG CAGAATAACTCAGAGCATGTCTTTGAAGATGCTGGGCGTCTACCACCACCTTATGTCCCCACCCTACCCA ACAAAAATAAGTAAAAAGAATATGGTGTATTCTACAAATTTGTGGCATGCTCAAAGTTTATGATCACATA AAGGCAAGAGGATACTTCATGAATAATACATTTCAATGCAAATAAACAGATGGTTCACTTCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094000 unedited GGTGCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTTTTTAGT AGAAAGGGGTTTCACCTTGTTGGTCAGGCTTGTCTCAAACTCCTGACCTCAGGTGATCCA CCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACACATGGCCGAAG GTTCTTCTTAAGTAGACATTCAGACTCACAAGGCACATCGCAGTTAACATCAGAATCACT TCTGATGATAATATAAGTGAAGAATATAAGACAGGAGGCGCATATATTAATACCAGCAGA GCAGCTCCCAGTGTGTCTCTTCAGTTGGAACAGTTGTTGCAGTGGTCTACTTGCTGTCCA GAAGCCTGATAAGAGAAAAAGATTCCCATGGAGAAATGTTCTTCGAAGTGATAACCATGC TTACTCATAAGGAGTTGAAATGTAGCTTACCTGCTAGTTTTCCTCCAATAAAAATGTGTT TATCTTTCATTCTGATTTGTTGTGAAGCTTTTGCACACTCTAATTTAAATCTTGGTAGCA TATATCTAGTTGAGTACCCACAGTGCACCAGGCTCTATTCCAGGGCCCAGGAAATGGAAG TCAGTAAGACACGTGGTTCAAGCCCTCCCTGAGACAGATGGTAGTACAGAATGGTATGTG GTATGATGTGCTGTAGCACAAGTTGCTGGTAGAGTGAAAAAGAAAGCTTCTACCCCAGCA CTATCAAACCAACTTCAGAAACAGTGAATTACAGGAAAGATTAGTACTTNCTTTTATATG ATCCATTGTTGAGTGTCAAGGAATTGNTATTAANTAACATCCTTGAATCTTTAGCCGACA TTTAACTGACTGTCATTGTAAGGACACTGTTTGAAGTACTTCACATGTATAAAAATTTCC ACTTAAACATACATGCGTTGTGAGAATGCCTCTTAGAA
Restriction Sites	NotI-NotI
ACCN	AK094000
Insert Size	6000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094000.1
RefSeq Size	2372 bp
RefSeq ORF	2372 bp
Locus ID	5599
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Protein Pathways	Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

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