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ADO (NM_032804) Human Untagged Clone
CAT＃: SC103692
ADO (untagged)-Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C10orf22 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC103692 sequence for NM_032804 edited (data generated by NextGen Sequencing)
ATGCCCCGAGACAACATGGCCTCCTTGATCCAACGGATCGCCCGCCAGGCTTGCCTCACC TTCCGGGGCAGCGGGGGCGGCCGCGGCGCTTCCGATCGCGACGCGGCTTCTGGCCCGGAG GCGCCGATGCAGCCGGGCTTCCCCGAGAACCTGAGCAAGCTGAAGAGCCTCCTGACCCAG CTCCGCGCCGAGGACTTGAACATCGCCCCGCGCAAGGCCACACTGCAGCCGCTGCCGCCC AACCTGCCGCCAGTCACCTACATGCACATCTACGAGACGGACGGCTTCAGCCTGGGCGTG TTCCTGCTCAAGAGCGGCACGTCCATCCCGCTGCACGACCACCCGGGCATGCACGGCATG CTCAAGGTGCTGTACGGCACCGTGCGCATCAGCTGCATGGACAAGCTAGACGCGGGCGGC GGGCAACGGCCGCGGGCCTTGCCGCCCGAGCAGCAGTTCGAGCCGCCGCTGCAGCCCCGG GAGCGAGAAGCCGTGCGGCCGGGCGTGCTGCGTTCGCGGGCCGAGTACACCGAGGCCAGC GGCCCCTGCATCCTCACACCGCACCGGGACAACCTGCACCAGATCGACGCCGTGGAAGGG CCTGCCGCCTTCCTGGACATCCTGGCCCCGCCCTACGACCCGGACGATGGCCGGGACTGC CACTATTACCGGGTGCTGGAGCCGGTCAGGCCCAAGGAGGCCTCCAGCTCGGCCTGTGAC CTGCCTCGAGAGGTGTGGCTCCTGGAGACCCCACAGGCCGATGACTTCTGGTGCGAGGGA GAACCCTATCCAGGTCCCAAGGTCTTCCCTTGA Clone variation with respect to NM_032804.5 >OriGene 5' read for NM_032804 unedited
CGCGTGCGCGCTCAGAGGGGGCTCAAGGCGAGCGCGCCGGGCAGTTGCGGGCGCGTGGCT GCTGAGGTTGGCGGCGGTGCCGCGCGCCCGACGGGCCGGTGGTTGCGGGGCCTCCCGCCT CGACCCGGGCTGGGGGCAGCCGTGGCGGCCGCCGGGGACCGCAAGGGGCGGAGGAAAGGA GGGGGCCGGTCCCGGCACGCAGAGGAGCAGCCGACCATGCCCCGAGACAACATGGCCTCC TTGATCCAACGGATCGCCCGCCAGGCTTGCCTCACCTTCCGGGGCAGCGGGGGCGGCCGC GGCGCTTCCGATCGCGACGCGGCTTCTGGCCCGGAGGCGCCGATGCAGCCGGGCTTCCCC GAGAACCTGAGCAAGCTGAAGAGCCTCCTGACCCAGCTCCGCGCCGAGGACTTGAACATC GCCCCGCGCAAGGCCACACTGCAGCCGCTGCCGCCCAACCTGCCGCCAGTCACCTACATG CACATCTACGAGACGGACGGCTTCAGCCTGGGCGTGTTCCTGCTCAAGAGCGGCACGTNC ATCCCGCTGCACGACCACCCGGGCATGCACGGCATGCTCAAGGTGCTGTACGGCACCGTG CGCATCAGCTGCATGGACAAGCTAGACGCGGGCGGCGGNCAACGGCCGCGGGCCTTGCCG NCCGAGCAGCAGTTCGAGCCGCCGCTGCAGCCCCGGGAGCGAAAACGGTGGCGCCNGGCG TGCTGCGTTCGCGGGCCGAGTACACCGAGGCCANNGGCCCCTGCTNCTCACACCGNACCG GGACACCTGCACANATCACACCCNGGGGAAAGCCCTGCGGCTTCCTGGC >OriGene 3' read for NM_032804 unedited
CTATGGAACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGAGGCAAT TATTGCATTTATTATTCACCATAAAAGTGATTAATATCATTGTGCTCATTAGTCTTTCCA AGGGGAAGATAACTTTTATCCCACAGTTGTTATTAAAACATTATCTATATTGGACGGAGT ATATAGAAACACAATAGCAACACACCAAGTCAGTCACGACTAACAGTTTATGCACACAGG TGCAAGATAGTTTCTGCACCACATTTTGTCTTTGTAATAAGCATATTCTTTCCTCAGTGG CTCACTAAAACAAGTTATATTTTGTCTTCACCACTAATTTACTCAGAATTTATTAGTGAA CCAAAAGTTTCACTGTTCTTATTGAACTAAATCTGAGATATCATATTGCTGTTTTGGCCT GCAACAATTTCAGTTACTCTTTTGAAAAGCACCTTAGGTTTTTTCTTCCAAATTGCTCGT AATTGAAAACTTTAAAACTTCTTTTTTTCATAATGAAGTCATAATTCTTCCTTTATTACT ATCCTACAGCCAGGATGAGTTTTCGTAGCTTTAATGTACTAAGTTGTATTATAACACATA CATTGCTATTAATAATCTTGGTAAGCTAATCAACTTTTTGCTACAATATAATCCAAAAAA GTATGTGATTGATAACCACATTCATTACTGTGCTATGGAAAAAAAAACAAGATCTTTTAT TTTGTGAAGTTTTCTAAGAAAATCATTTGCATATCTAAGAATTTATTTTCAAACTAAGTG GCATGAAGAACTGCCTCAAACTGCCCCCAAAGGGCATTTTAAACTACCCCAAAATGTGGA AGCTACCATAAAACACATTTAGCCACAATGGCGGGAAAAACTTGGTACATGGCCCGTGGT CGGGTTTATAAAAAAGTGGCTGGCCCGGGGGAAAACCTTTTGGGTTTGGTTTGGGGTAAA TTAAAAACACTCACAGTGTTTGCGTGTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032804 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032804.5, NP_116193.2 |
| RefSeq Size | 3739 bp |
| RefSeq ORF | 813 bp |
| Locus ID | 84890 |
| UniProt ID | Q96SZ5 |
| Protein Pathways | Metabolic pathways, Taurine and hypotaurine metabolism |
| Gene Summary | Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied by OMIM, Mar 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205583 | ADO (Myc-DDK-tagged)-Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO) |
CNY 3,600.00 |
|
| RC205583L1 | Lenti ORF clone of Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC205583L2 | Lenti ORF clone of Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), mGFP tagged |
CNY 5,890.00 |
|
| RC205583L3 | Lenti ORF clone of Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205583L4 | Lenti ORF clone of Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), mGFP tagged |
CNY 5,890.00 |
|
| RG205583 | ADO (tGFP-tagged) - Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO) |
CNY 5,200.00 |
