FAM178A (SLF2) (BC015131) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C10orf6; FAM178A
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC015131, the custom clone sequence may differ by one or more nucleotides GGGGGAAGAGAGAACCGCCATGAAGAGAGAAGGGGGTGCCGCCCACCTCTGCTCCGACAGCCTCCCGGAG TCCCAGCAGCAAGACGGCAACCACGCACCCAACTTCTCCAGCCACGGCTCATGCCGCCGTCGCCAGCGGC GCCGACATGACAAGGCGCTGCATGCCCGCTAGGCCAGGTTTCCCCTCATCCCCAGCCCCGGGGTCGTCGC CCCCGCGCTGCCATCTGAGACCCGGTAGTACCGCCCATGCTGCAGCGGGAAAGAGAACAGAGAGTCCTGG GGACAGGAAGCAGTCAATTATAGATTTCTTCAAACCAGCTTCAAAACAAGACAGACACATGTTGGATTCA CCACAAAAATCAAACATCAAATATGGAGGAAGTAGATTGTCTATCACTGGGACAGAGCAGTTTGAAAGGA AACTATCCTCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015131 unedited GGCGGCCCGCGAATTCGCACGAGGGGATAGCCGCCATGAAGAGAGAAGGGGTGCCGCCCA CCTCTGCTCCGACAGCCTCCCGGAGTCCCAGCAGCAAGACGGCAACCACGCACCCAACTT CTCCAGCCACGGCTCATGCCGCCGTCGCCAGCGGCGCCGACATGACAAGGCGCTGCATGC CCGCTAGGCCAGGTTTCCCCTCATCCCCAGCCCCGGGGTCGTCGCCCCCGCGCTGCCATC TGAGACCCGGTAGTACCGCCCATGCTGCAGCGGGAAAGAGAACAGAGAGTCCTGGGGACA GGAAGCAGTCAATTATAGATTTCTTCAAACCAGCTTCAAAACAAGACAGACACATGTTGG ATTCACCACAAAAATCAAACATCAAATATGGAGGAAGTAGATTGTCTATCACTGGGACAG AGCAGTTTGAAAGGAAACTATCCTCACCAAAAGAATCTAAACCCAAAAGGGTGCCACCAG AAAAGAGCCCTATTATAGAAGCTTTCATGAAAGGTGTTAAAGAGCACCATGAAGATCATG GTATACATGAGTCACGTCGGCCTTGTCTGTCACTAGCCTCCAAATATTTAGCCAAAGGAA CAAATATCTATGTTCCTTCTTCATATCACTTGCCAAAGGAGATGAAGTCACTAAAGAAAA AACATCGATCCCCAGAGAGAAGAAGTCACTATTCATTCATGANNAATATGAGGAGAATGA TAGAGATCGAGGGCAAACCCATGCAGACTCCAAAAGCAGACACAGTGGCAGAAGCTGACA TCTNAATAACAGCTNCAGAAGCCTTAACAGCAGGAGCAGCCTGTCCAGNCACCACCCGGA GAAACCCACTGGGAGCTAATTCCAGTTGCACTAGCTTCTACTGCANAGACGAGACTAAAG AGGTGAGAAGGAGCAATGG
Restriction Sites	NotI-NotI
ACCN	BC015131
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC015131.1
RefSeq Size	463 bp
RefSeq ORF	463 bp
Locus ID	55719
Gene Summary	Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).[UniProtKB/Swiss-Prot Function]

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