Retinoblastoma binding protein 6 (RBBP6) (AK023612) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MY038; P2P-R; PACT; RBQ-1; SNAMA
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023612, the custom clone sequence may differ by one or more nucleotides TGTATGAGAGAATAAACAAACTTACTCAGACTGAAGAGTATGTTTTCAAAAATTAAAGAAGTAGCAATTA TATGGGAGCTGTTATTTATTAAAGCAATTATCTGAGCCCTTAAAGGGCTGTTTGTTTTTACAATGTTGTA TAATGGCCTTGCAGGAAAATTAGATTTCTTATTCTCTCTTCCAGTGTACATTGTCTTGAGTATTGTCACA TTTAGAGACCATAGGGAGATATGGGACTCCTAGTATTCAGTCGACACTCTGAACAGATTTTCTGTGTGAT ACATTTTTTCATTTTAATTGAGTCCCCTCTTCCTCCCCTCCAACTGATAGCTTTTTATAACTGTGTGAAA TATCTAGAGAAGAATGAAGTAGGCTGAGCTACATTTTCAAGCTTAACATTCTTTAGAAAACTAAGAAAAT GCCCAATATTTCAACATTAAGGTGTCAATAGGGGCTAAATGATCACTTTAAGATGTTTTCAGTTTGGAAC TTGAATGTGTGTTTAATTTTTTGTGAACAGATTGATGACTCTTCCGCGTCTATTTCTCTGGCCCAGCTTA CAAAGGTATATATATATATATATTCTTGAAAATATAAGTTTTTTTCTAACATCATATTTTTTTATGTGGA ACGGTTTTTTAAGAGGGGTTGGGGGAGGACATATTTTGCAAAAGCATTGTACATATTATTACTATCCTGT AAAGAGTAGTTGCTGTTTTGAGAAGATAGGGGAATACTTTGTCAGCCTCAGCGTATTGGAGTATTGTTCA TCTTCAAGACAGAAGAAACACTTGAAGCTACACTTAATTATTTTTTTTTCCCTCATACATTTTGTTTAAT TATACATTGGAAACTAACAAGGTTCTGGAGGAATTTCCTGGTTTTGGCTATAAATTATGTGGACCTTTTT TAAGCAGCCAAAACAGAAGATTTCCTTTACACGGACGATTGCATGGTTATTTCTTGAATATTTGACTTGA TCCCATGAAGGTCAATATTTTAAATGTGATGCAGTTGTTTCTTTATGCTATAGACTTGTAGTCTGTCTGC ATTAGGAAAAGTTTTGAACAACCTAATTTGTTTGAACACAGACTACATGAATTGTGTAAGTGTGTAAAGC TGTTTGCAGGAATATACCAATGTGTATGTTTGGCAGAGGGGCAAATTGTTACCTCCTGAATCACTGTATA TGCCATGTTTTGCGATAAGATTGCTTGCATTTTCTGCTCAACAATGTGTATCTTCTGTTTGGGAAAGCAC TAGTGATGGATTACTTTTTAAAGCAATACATTTAGCTTGCAAATTGTGCCTTTAAAAAAAAAAATAGGCA GACTTTTGAGGGCCAAGAAGGAAGCTGTCCAGTTTTCCAAAAATCCTTTTTCCCTGCTATCAGAAATGTG AAACCAAATTTAGCAACCAAGATTAATGAAAAGATGGGTTTTCCATTAGTGCTGTCCCTATCTTGTTCTT GGCTTTGTTATGTCCTTTCCCCTAGACTGTATCCCGACAAAATGTCCTAGTAACAAATTGCTTTTTAAGC TCCTGTTCTGGGAAAACTAAGCATTAAAATTGATTATTCTAAAACATAAAGTGGACTAAAGCCATCCTAT TTTATAATTTTCTAATGCAAAGTGGTTTAGTATAGAGTTAACACTTAGAAGTTTATAGTTTACTGTTTTT ATTCTTATGTACTGTAAGGACCATATTTGAGTTTTTGGTCTATTCCTACCATTGTTTCTTTGTGGGGAGG AGTTGGGGCGGTTTGGGGGATTGGTTTTTTTTTTTTGTTTTTTTAAACTACAGGTATTTGTAAAACAATG TTTGGGTTCAAACAAATTAGTTGTTAAACATCTGTAATCCAGTTTTCTGTAAATGTTGCTGTTGTTCTAA GCTCTGTTAATGTTAAGCATTCTTTGTATATAAAATTACAATAAAATGTTAAAACTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023612 unedited GTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCCC TCCAACTGATAGCTTTTTATAACTGTGTGAAATATCTAGAGAAGAATGAAGTAGGCTGAG CTACATTTTCAAGCTTAACATTCTTTAGAAAACTAAGAAAATGCCCAATATTTCAACATT AAGGTGTCAATAGGGGCTAAATGATCACTTTAAGATGTTTTCAGTTTGGAACTTGAATGT GTGTTTAATTTTTTGTGAACAGATTGATGACTCTTCCGCGTCTATTTCTCTGGCCCAGCT TACAAAGGTATATATATATATATATTCTTGAAAATATAAGTTTTTTTCTAACATCATATT TTTTTATGTGGAACGGTTTTTTAAGAGGGGTTGGGGGAGGACATATTTTGCAAAAGCATT GTACATATTATTACTATCCTGTAAAGAGTAGTTGCTGTTTTGAGAAGATAGGGGAATACT TTGTCAGCCTCAGCGTATTGGAGTATTGTTCATCTTCAAGACAGAAGAAACACTTGAAGC TACACTTAATTATTTTTTTTCCCTCATACATTTTGTTTAATTATACATTGGAAACTAACA AGGTTCTGGAGGAATTTCCTGGTTTTGGCTATAAATTATGTGGACCTTTTTTAAGCAGCC AAAACAGAAGATTTCCTTTACACGGACGATTGCATGGTTATTTCTTGAATATTTGACTTG ATCCCATGAAGGTCAATATTTTAAATGTGATGCAGTTGTTTCTTTATGCTATAGACTTGT AGTCTGTCTGCATTANGAAAAAGTTTGAACAACCTAATTTGTTTGACACAGACTACTGAA TTGTGTAAGTGTGTAAAGCTGTTTGCAGGATATACCAATGTGTATGTTTGGCAGAGGGCA AATNGNTACTNCTGAATACTGNATATGCCATGTTTGC
Restriction Sites	NotI-NotI
ACCN	AK023612
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023612.1
RefSeq Size	1947 bp
RefSeq ORF	1947 bp
Locus ID	5930
Protein Families	Druggable Genome
Gene Summary	The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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