Glutamine Synthetase (GLUL) (AK092874) Human Untagged Clone

CAT#: SC103453

(untagged)-Human cDNA FLJ35555 fis, clone SPLEN2004773, highly similar to GLUTAMINE SYNTHETASE (EC 6.3.1.2)



CNY 5,528.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
GLUL mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GLNS; GS; PIG43; PIG59
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for AK092874 edited
GAATTCGGCACGAGGGTTTTGTAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAG
TTTAGTATTTGGATAGTTTAGTGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCC
TCACAAGTATAAGTTCTAGCTTGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCC
TCTGAGCTCAGGGATGCAGAGGCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGC
AGGCATCTGTGTTAATTGTTTCAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGA
GATTTTTGCCTACTATAAAACTAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTG
TGTTGACTTCCACAAGGGAAGGAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCC
TTCTTGAGCCATTTTTGCAGGGGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTT
TTTCTTCTCCAGAACACCTTCCACCATGACCACCTCAGCAAGTTCCCACTTAAATAAAGG
CATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGAGAAAGTCCAGGCCATGTATATCTG
GATCGATGGTACTGGAGAAGGACTGCGCTGCAAGACCCGGACCCTGGACAGTGAGCCCAA
GTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGG
TTCCAACAGTGACATGTATCTCGTGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGA
CCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTACAATCGAAGGCCTGCAGGTGTGTT
ATAGCACAGCTATGGATACCCCTCCTCAATCTGTGAATGCTGTGAAGGGGAGGGAGAAGA
CATTCTGAAATCAGCATTGGGAAGACTAGGCAATTTCAGCACTATTTTAAGAATCTGAGT
GATTCTTTTCCCTGAACTTCTGCTTTGAGGAAGAGATAATATGGCCCATCTTTCTATGGT
CTTCTCTGTTGGTTGCATAAAATAGCATTGGATTTGTCCAGATCTGTTTGCCGGTCTTGG
AGTCCCCAGTAACAGCCTTCCTGCCTGGAATGTAGGCCAGGACAAATGTAAACCAATGGA
CAAATGTTTCTCAAAAATTATAGAATGGCTCCAAGTGCCTGAGAAATGAAGAATAAATCT
GACAACCAGAAGCAGCTGTCTTGTGAATAGAGGGTTAAGTGCCTGGCATTTGGTGCTTGG
GAGGTGGCCAGAATGCAGATAAGGTGAAAGTTGCCCTGTTCTAAATCCACTCCCATGTGA
CTTGGTTGTAACTGAGTTTAGTTAAAACTGAAGTCTTTCAGAGTCTTCCTACAGATGTAC
AATTAACAGCTTCTCTCATTTTTCTGACTCGGTGATCCCAAGAAGGCCTATACTGGGTCA
GTTCATACCATAGTGCACACCTCAGTTGTATAGAATCCAAGGACTATTCTCCCATCAGCA
TCGGTATTCAGCATCTATGTCTTTAGATCCCTGATGGCGTATTATTGACTCTTTTTTCTA
GAGACCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGTGAGCAACCAGCACCCC
TGGTTTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGGGCACCCCTTTGGTTGG
CCTTCCAACGGCTTCCCAGGGCCCCAGGGTAAGTCTCCTTGGGTTAGAGGTGAAATTCCC
AGAAGTGTCTAACTGTGCAGGAATGCCCCTTCCCAGGGATGGGAATGACTTTCAGAATCA
AGAAGCAAAATAATACAGTAAAGGCGAAACAGCCCTCACATCACCAAAGTCCAAAAATGG
ATATGAATATATAAAGTAAGGTTTTAGGGGGAACGTTTGGCCCCACTGAAGCTGTGGTGA
AGAGGAACTCCCCTATTGCCCCTCCCCTGCCCCGCACCTGCAGATGAAGGCAAGGATAGT
GATTCAAGAGGGCAAGGCTTAAGGGCCTTCTGATCTCTGACTTTGGGATTCTCTGGATTT
CTTGACTCTTAGCGTTTTGTCCTGATGCTTCTGTAGGTCCATATTACTGTGGTGTGGGAG
CAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTTGTATGCTG
GAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCCAGGTAAGTATAGCTCCAA
TCCATCAATGAAGAAGGGTAGGTAGGTGCACATAGGACTTTTGCTAGTAAGGGCTGCTGA
TACACCACTCACTAACCCAAAACCTAAGAACGGGTTGGAGTACAGGTGAGAAGAGAACAG
GTTTAGGAGATTCTGAGTTGGAGTGAGCAGTTAGCTTTGTTTTAATGGCCAAGCTTCTCG
TTTCTAGTGGGAATTTCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTG
GGTGGCCCGTTTCATCTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGA
TCCTAAGCCCATTCCTGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAA
GGCCATGCGGGAGGAGAATGGTCTGAAGTGAGTACCTTCTGCTGGGGCCATCTTTAATCT
CCTGTGGCAGAAAACTTGGGAGGAGACTTAGCAATCTCTCAGCAAAGTCTCCTTTGCAGG
ATGACTTGCAAATATTTGCCAAAGATGAGTAAACTTGACTTCTCAGTCTGGACGTACTTT
AGGTGTTGACACTTGCCTTCACATTCTCTCATTTTGTTCCTATTTGAAAAATACCAAATA
ATACTTCTGATTCACAGTGATAAATATTTGTTATAATTTATATAATATATATTAGTCATA
TATCATTATATAAATATATATCGATATATATATTTGTGACATGTCATGGTGACAGGGAAA
AGTTGACAAATTCATGCATTTGAAAATCTTTTAGAACTAAATTAGTAACAATACAGGCAT
GTGGATAAGCTTAATGCTTATGAGGGGGAGAAAGTTTCAAATGATTAGTCTTTTCAACAA
ACAGTAACTTTGTACTGCTTGTCGGGCACTGTTCTCACCACTGAGACACACAGGTAAGAA
GATGCAGCCACTGCCCTCATGAAGTATTTGTTCTACTGGTATCATATTTTGGTGCACTTC
ATTCTTGGCTCCATACCTGGAGACAAGGTTGGACTGCCATCTTTTCTGTTTACTCTAGGT
ACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGCACCAGTACCACATCCGTGCCTATG
ATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAACTGGATTCCATGAAACCTCCAACA
TCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCGCCAGCATACGCATTCCCCGGACTG
TTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTCGCCCCTCTGCCAACTGCGACCCCT
TTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTCTCAATGAAACCGGCGATGAGCCCT
TCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCTGTTGAGCCCCTCCTAGTTCTTCAT
CCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCGATTGTAACTCAAAGGGTGGAATAT
CAAGGTCGTTTTTTTCATTCCATGTGCCCAGTTAATCTTGCTTTCTTTGTTTGGCTGGGA
TAGAGGGGTCAAGTTATTAATTTCTTCACACCTACCCTCCTTTTTTTTCCCTATCACTGA
AGCTTTTTAGTGCATTAGTGGGGAGGAGGGTGGGGAGACATAACCACTGCTTCCATTTAA
TGGGGTGCACCTGTCCAATAGGCGTAGCTATCCGGACAGAGCACGTTTGCAGAAGGGGGA
CTCTTCTTCCAGGTAGCTGAAAGGGGAAGACCTGACGTACTCTGGTTAGGTTAGGACTTG
CCCTCGTGGTGGAAACTTTTCTTAAAAAGTTATAACCAACTTTTCTATTAAAAGTGGGAA
TTAGGAGAGAAGGTAGGGGTTGGGAATCAGAGAGAATGGCTTTGGTCTCTTGCTTGTGGG
ACTAGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTAGTATAAACTGATG
GATATCCCTACCTTGAAAGAAGAAAAGGTTCTTACTGCTTGGTCCTTGATTTATCACACA
AAGCAGAATAGTATTTTTATATTTAAATGTAAAGACAAAAAACTATATGTATGGTTTTGT
GGATTATGTGTGTTTTGCTAAAGGAAAAAACCATCCAGGTCACGGGGCACCAAATTTGAG
ACAAATAGTCGGATTAGAAATAAAGCATCTCATTTTGAGTAGAGAGCAAGGGAAGTGGTT
CTTAGATGGTGATCTGGGATTAGGCCCTCAAGACCCTTTTGGGTTTCTGCCCTGCCCACC
CTCTGGAGAAGGTGGGCACTGGATTAGTTAACAGACGACACGTTACTAGCAGTCACTTGA
TCTCCGTGGCTTTGGTTTAAAAGACACACTTGTCCACATAGGTTTAGAGATAAGAGTTGG
CTGGTCAACTTGAGCATGTTACTGACAGAGGGGGTATTGGGGTTATTTTCTGGTAGGAAT
AGCATGTCACTAAAGCAGGCCTTTTGATATTAAATTTTTTAAAAAGCAAAATTATAGAAG
TTTAGATTTTAATCAAATTTGTAGGGTTTCTAGGTAATTTTTACAGAATTGCTTGTTTGC
TTCAACTGTCTCCTACCTCTGCTCTTGGAGGAGATGGGGACAGGGCTGGAGTCAAAACAC
TTGTAATTTTGTATCTTGATGTCTTTGTTAAGACTGCTGAAGAATTATTTTTTTTCTTTT
ATAATAAGGAATAAACCCCACCTTTATTCCTTCATTTCATCTACCATTTTCTGGTTCTTG
TGTTGGCTGTGGCAGGCCAGCTGTGGTTTTCTTTTGCCATGACAACTTCTAATTGCCATG
TACAGTATGTTCAAAGTCAAATAACTCCTCATTGTAAACAAACTGTGTAACTGCCCAAAG
CAGCACTTATAAATCAGCCTAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAACTCGAC
>OriGene 5' read for AK092874 unedited
NGGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGTTTTG
TAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAGTTTAGTATTTGGATAGTTTAG
TGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCCTCACAAGTATAAGTTCTAGCT
TGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCCTCTGAGCTCAGGGATGCAGAG
GCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGCAGGCATCTGTGTTAATTGTTT
CAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGAGATTTTTGCCTACTATAAAAC
TAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTGTGTTGACTTCCACAAGGGAAG
GAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCCTTCTTGAGCCATTTTTGCAGG
GGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTTTTTCTTCTCCAGAACACCTTC
CACCATGACCACCTCAGCAAGTCCCACTTAAATAAAGGCATCAAGCANGTGTACATGTCC
CTGCCTCAGGNTGAGAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGA
CTGCGCTGCAAGACCCCGACCTGGACAGTGAGCCCAAGTGTGTGGAAGAAGTGCCTGAGT
GGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCG
TGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGGTGTATGTG
AAGTTTTCAAGTACAATCGAAGGCCTGCNNAGTGTGTATAGCACAGCTATGGATACCCCT
NCTCATCTGTATGCCTGTGTGAAGGG
Restriction Sites NotI-NotI     
ACCN AK092874
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AK092874.1, BAG52617.1
RefSeq Size 2265 bp
RefSeq ORF 2265 bp
Locus ID 2752
Domains gln-synt_N
Protein Pathways Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism
Gene Summary The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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