SNX14 (AK000362) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RGS-PX2; SCAR20
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000362, the custom clone sequence may differ by one or more nucleotides AAAAAACTGCCGGTAAGCGTCTGTGTGCGCCGCCAAGTCGGTGGGGCGGGGACGCGAGGTGTGGATGGGG GGTCGCCTTGACCTCTGCCTCAGCCAGTAGCGCAGCCTCGGCCTCGCCGTTACGGAGATGGTGCCCTGGG TGCGGACGATGGGGCAGAAGCTGAAGCAGCGGCTGCGACTGGACGTGGGACGCGAGATCTGCCGCCAGTA CCCGCTGTTCTGCTTCCTGCTGCTCTGTCTCAGCGCCGCCTCCCTGCTTCTTAACAGGTATATTCATATT TTAATGATCTTCTGGTCATTTGTTGCTGGAGTTGTCACATTCTACTGCTCACTAGGACCTGATTCTCTCT TACCAAATATATTCTTCACAATAAAATACAAACCCAAGCAGTTAGGACTTCAGGAATTATTTCCTCAAGG TCATAGCTGTGCTGTTTGTGGTAAAGTGAAATGTAAACGACATAGGCCTTCTTTGCTACTTGAAAACTAC CAGCCATGGCTAGACCTGAAAATTTCTTCCAAGGTTGATGCATCTCTCTCAGAGGTTCTTGAATTAGTGT TGGAAAACTTTGTTTATCCGTGGTACAGGGATGTGACAGATGATGAATCCTTTGTTGATGAACTGAGAAT AACATTACGTTTTTTTGCATCTGTCTTAATAAGAAGGATTCACAAGGTGGATATTCCATCTATTATAACC AAGAAACTATTAAAAGCAGCAATGAAGCATATAGAAGTGATAGTTAAAGCCAGACAGAAAGTAAAAAATA CAGAGTTTTTACAGCAAGCTGCTTTAGAAGAATATGGTCCAGAGCTTCATGTTGCTTTGAGAAGTCGAAG AGATGAATTGCACTATTTAAGGAAACTTACTGAACTGCTTTTTCCTTATATTTTGCCTCCTAAAGCAACA GACCGCAGATCTCTGACCTTACTTATAAGAGAGATTCTGTCTGGCTCTGTGTTCCTTCCTTCTTTGGATT TCCTAGCTGATCCAGATACTGTGAATCATTTGCTTATCATCTTCATAGATGACAGTCCACCTGAAAAAGC AACTGAACCGGCTTCTCCTTTGGTTCCATTCTTGCAGAAATTTGCAGAACCTAGAAATAAAAAGCCATCT GTGCTGAAGTTAGAATTGAAGCAAATCAGAGAGCAACAAGATCTTTTATTTCGTTTTATGAACTTTCTGA AACAAGAAGGCGCAGTGCACGTGTTGCAGTTTTGTTTGACTGTGGAGGAATTTAATGATAGAATTTTACG ACCAGAATTATCAAATGATGAAATGCTGTCTCTTCATGAAGAATTGCAGAAGATTTATAAAACATACTGT TTGGATGAAAGTATTGACAAAATTAGATTTGATCCCTTCATTGTAGAAGAGATTCAAAGAATTGCTGAAG GCCCACACATAGATGTTGTGAAACTTCAAACTATGAGATGTCTTTTTGAAGCATATGAACATGTTCTTTC CCTTTTGGAGAATGTATTTACTCCTATGTTCTGCCATAGTGATGAGTATTTCAGACAACTTTTAAGAGGT GCAGAATCACCAACACGCAATTCAAAATTGAACAGAGGTAGCCTAAGTTTGGATGATTTTCGGAACACAC AGAAAAGGGGAGAATCATTTGGAATCAGCAGAATAGGTAGCAAAATTAAAGGAGTATTCAGAAGTACCAC AATGGAGGGAGCTATGTTGCCTAATTATGGTGTAGCTGAAGGTGAAGATGATTTTATTGAAGAAGGTATT GTTGTAATGGAAGATGATTCTCCAGTGGAGGCTGTGAGCACACCTAATACTCCCCGAAACCTTGCTGCAT GGAAAATTAGCATTCCATATGTAGACTTTTTTGAGGATCCCTCCTCTGAAAGGAAGGAGAAAAAAGAAAG AATTCCTGTGTTTTGTATTGATGTTGAAAGAAATGATAGAAGAGCAGTTGGACACGAGCCTGAACATTGG TCTGTCTATAGAAGATATCTTGAATTCTATGTACTTGAATCAAAACTAACAGAATTTCATGGTGCATTTC CTGATGCCCAGCTTCCTTCTAAGAGGATCATTGGCCCCAAAAATTATGAATTCTTAAAGTCAAAGAGGGA AGAGTTCCAAGAATATCTACAGAAACTTCTGCAGCATCCAGAACTGAGTAATAGTCAACTTCTGGCAGAC TTTCTTTCCCCTAATGGTGGGGAAACACAATTTCTTGATAAGATACTACCAGATGTAAATCTTGGGAAAA TTATAAAATCTGTTCCTGGAAAACTAATGAAAGAGAAAGGTCAGCATTTGGAACCTTTTATCATGAATTT CATTAATTCTTGTGAGTCTCCAAAGCCTAAACCAAGTAGACCAGAACTGACCATTCTCAGCCCTACTTCA GAAAACAACAAGAAGCTTTTCAATGATCTGTTTAAAAATAATGCAAACCGTGCTGAAAATACAGAGAGAA AGCAAAATCAGAATTATTTTATGGAGGTGATGACTGTAGAAGGAGTCTATGATTACCTGATGTATGTAGG ACGGGTAGTTTTCCAGATTCCTGACTGGCTTCATCATCTCTTAATGGGAACTCGAATCCTCTTTAAAAAC ACCCTGGAAATGTATACTGATTACTATCTTCAGTGTAAACTAGAACAGCTATTTCAGGAGCACCGTTTGG TCTCACTCATAACACTTCTCAGAGATGCTATATTCTGTGAAAACACTGAACCTCGCTCTCTCCAAGATAA GCAAAAAGGAGCAAAACAGACTTTTGAAGAAATGATGAATTACATTCCAGATCTGTTAGTCAAGTGTATT GGTGAGGAAACCAAGTATGAAAGCATCAGACTTCTGTTTGATGGCTTACAGCAACCAGTACTCAACAAGC AGCTGACTTATGTTTTATTGGACATTGTGATACAGGAACTGTTTCCAGAGCTCAATAAGGTACAAAAGGA AGTTACCTCTGTGACATCTTGGATGTAAACACTTGGATTTGGTATAGAATAACCCATTGAAATTTCTGCT GTGCGAGGGTGGTAGAAATTTACTTTTTTGGGTATATTCTATATATATTATGTACATCGCTGTCTGAAAT TTTAGTTATTTTTTGTTTTTAATAAAGACTAACACAACTTAATGATTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000362 unedited TATAGGCGGGCCGCGAATTCGCACGAGGCTCTCCTCCGCATACAGGCAGTTACTGGGTGC TGGGGCTGTCCTTTGATTTCCGGAAATGGCCGGTCGCCAGTGTTGTCAGAGTTTCCACGG AAGACTGCGCGCTACTCCGGCCAGGAGGGCGGAGCGGCGAGACGCTAGAGGCTTTGCGCT GGGTGACGACTTCGGGCACGTTCTACGGCCGTTCCTCTGGTCGGCTGGGGACCGCGGCCG CGCACGCGCGGCGGGGGGCGTGGGGCTGGGCCCAGCCGGACGCGACCTCAGCCTGCGGCG GCTAACTGCCGGTAGGCGTCTGTGTGCGCCGCCAAGTCGGTGGGGCGGGGACGCGAGGTG TGGATGGGGGGTCGCCTTGACCTCTGCCTCAGCCAGTAGCGCAGTCTCGGCCTCGCCGTT ACGGAGATGGTGCCCTGGGTGCGGACGATGGGGCAGAAGCTGAAGCAGCGGCTGCGACTG GACGTGGGACGCGAGATCTGCCGCCAGTACCCGCTGTTCTGCTTCCTGCTGCTCTGTCTC AGCGCCGCCTCCCTGCTTCTTAACAGGTATATTCATATTTTAATGATCTTCTGGTCATTT GTTGCTGGAGTTGTCACATTCTACTGCTCACTANGACCTGATTCTCTCTTACCAAATATA TTCTTCACAATAAAATACANACCCAAGCAGTTAGGACTTCAGGAATTATTTCCTCAAGGC ATAGCTGGGCTGTTTGTGGGAAAGTGAAATGTAAACGACATAGGCCCTTCTTTGCTACTT GAAAACTACAGCCATGGGCTAGACCTGAAATTCTTNCAGGNTGATGCTCCTTTCCAAGGT CTTGGAAATAAGGTGGGAAAACTTGGTATCCGGGGCCCGGGAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK000362 unedited AACGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTAATCAACAAGTTTGTGT TAGTCTTTGTTAAAAACGAGAAATACCTAAAATTTGTGACAGCGTTGTACATAATATATA TAAGAATATACCCCAAATAGTAAATTTATACCACCCTCGCACAGCAGAAATTTCAATGGG TTATTCTATACCAAATCCAAGTGTTTACTTCTCTATATGTCACAGAGGTAACTTCCTTTT GTACCTTATTGAGCTCTGGAAACAGTTCCTGTATCACAATGTTCAATAAAACATAAGTCA GCTGCTTGTTGAGTACTGGTTGCTGTAAGCCATCAAACAGAAGTCTGATGCTTTCATACT TGGTTTCTTCACCAATACCCTTGTCTAACACATCTAAACCAAAAGGCCAAATGTGAAATA ATTTTATATTATAAAGTGACTCACCCTTAACTGGATCATAAAGCAGAATCACCACCCTGT TTCCCAGATACTAGGCAAAAACTGCTAATCAATACTCCCCAAACCAGTGTTTCTATAAGA GGCTCCTGGAAAGAGGTTTCTATGGGCAAGTTGACGAATGCAGTATCATGGTTTCCATTT TTGGAGATTCACAATGCACAAGGCATAATGCACACTCTGAGAAATGCTGGAATGTAATTC ATCATTCTCTCAAAAGTCTGTTTTGCCCCTTTTTGCTTATTTTGGAGAAACCGAGGTTCT GTGTTTTCACCGAATATAGCATTTCTCAAAAATGTTATGAGTGAGACCAACCGGGCTCCT GAAAAACCGGTTTAGTTAACCTGGAGAAAGGAACCAGTTACCTTTTCCAGGGGGTTGTAA AAGGAATCGGGTCGCGTTAAAAATGAATTAAGCCTTTGGGACCTGGAAACTACCGTCTAC ATTTTGGGAATATAGCTCTTTCCAGCATACCTCCGAAAAATTTTAATTGCGTTCTGGGAA TTAAGCGGCTGGTTTTTTAAAAACATGGAAACTT
Restriction Sites	NotI-NotI
ACCN	AK000362
Insert Size	4100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK000362.1
RefSeq Size	3145 bp
RefSeq ORF	3145 bp
Locus ID	57231
Domains	RGS, PX, PXA
Protein Families	Transmembrane
Gene Summary	This gene encodes a member of the sorting nexin family. Members of this family have a phox (PX) phosphoinositide binding domain and are involved in intracellular trafficking. The encoded protein also contains a regulator of G protein signaling (RGS) domain. Regulator of G protein signaling family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. Alternate splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

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