Laminin (LAMA1) (AK091949) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LAMA; PTBHS; S-LAM-alpha
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103296 sequence for AK091949 edited (data generated by NextGen Sequencing) ATGCTGATCGGAGGCAACATTGAGGTACATGTCAATCCTGGGGATGGGACAGGCCTGAGA AAAGCTCTCCTGCACGCTCCCACGGGTACCTGCAGTGATGGACAAGCGCATTCCATCTCC TTGGTCAGGAATCGGAGAATTATCACTGTCCAATTGGATGAGAACAATCCTGTGGAAATG AAGTTGGGCACATTAGTAGAAAGCAGGACGATAAATGTGTCCAATCTGTACGTCGGGGGA ATTCCAGAGGGAGAGGGGACGTCACTGCTCACAATGAGAAGATCGTTCCATGGCTGTATC AAAAACCTGATCTTCAATTTGGAACTTTTGGATTTCAACAGTGCAGTTGGCCATGAGCAA GTCGACCTGGACACCTGCTGGCTGTCAGAAAGGCCTAAGCTGGCTCCCGATGCAGAGGAC AGCAAGCTCTTGCCAGAGCCCCGGGCTTTTCCAGAACAGTGTGTGGTGGATGCAGCTCTG GAGTACGTTCCCGGCGCTCACCAGTTTGGTCTCACACAAAACAGCCATTTCATCTTGCCT TTTAATCAGTCGGCTGTCAGAAAGAAGCTCTCGGTTGAGCTAAGCATCCGCACGTTCGCC TCCAGCGGCCTGATTTACTACATGGCTCATCAGAACCAAGCAGACTACGCTGTGCTCCAG CTGCACGGGGGCCGCCTCCACTTCATGTTTGACCTTGGCAAAGGCAGAACAAAGGTCTCT CACCCTGCACTGCTCAGTGATGGCAAGTGGCACACGGTCAAGACAGACTATGTTAAAAGA AAAGGCTTCATAACTGTCGACGGCCGAGAGTCTCCCATGGTGACTGTGGTGGGAGATGGA ACCATGCTGGATGTGGAGGGTTTGTTCTACCTAGGAGGCCTGCCCTCCCAGTACCAGGCC AGGAAAATTGGAAATATCACCCACAGCATCCCTGCCTGCATTGGGGATGTGACGGTTAAC AGCAAACAGCTGGACAAGGACAGCCCGGTGTCTGCCTTCACGGTGAACAGGTGCTACGCA GTGGCCCAGGAAGGAACATACTTTGACGGAAGCGGATATGCAGCTCTTGTCAAAGAGGGC TACAAAGTCCAGTCAGATGTGAACATCACACTGGAGTTTCGAACCTCCTCGCAGAATGGC GTCCTCCTGGGGATCAGCACTGCCAAAGTGGATGCCATTGGACTAGAGCTTGTGGACGGC AAGGTCTTGTTCCATGTCAACAATGGTGCTGGCAGGATAACAGCTGCATATGAGCCCAAA ACCGCCACTGTGCTCTGTGATGGAAAATGGCACACTCTTCAAGCTAACAAAAGCAAACAC CGTATCACTCTGATTGTTGACGGGAACGCAGTTGGCGCTGAAAGTCCACACACCCAGTCT ACCTCAGTGGACACCAACAATCCCATTTATGTTGGTGGCTATCCTGCTGGTGTGAAGCAA AAATGCCTGCGCAGCCAGACCTCGTTCCGCGGGTGTTTGAGGAAGCTAGCTCTGATTAAG AGCCCGCAGGTGCAGTCCTTTGACTTCAGCAGAGCGTTCGAACTGCACGGAGTTTTCCTT CATTCCTGTCCTGGGACNGAGTCCTGA Clone variation with respect to AK091949.1 1450 t=>c;1578 c=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091949 unedited CCATATGGATCCGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGCTACATTG AATTGCCACCCAAATCTTTGTCACCAGAATCAGAATGGCTGGTAACATTTGCCACCACGA ACAGCAGTGGCATCATCCTGGCTGCCCTCGGCGGGGATGTGGAGAAGCGGGGTGACCGTG AGGAAGCACACGTGCCCTTCTTTTCCGTCATGCTGATCGGAGGCAACATTGAGGTACATG TCAATCCTGGGGATGGGACAGGCCTGAGAAAAGCTCTCCTGCACGCTCCCACGGGTACCT GCAGTGATGGACAAGCGCATTCCATCTCCTTGGTCAGGAATCGGAGAATTATCACTGTCC AATTGGATGAGAACAATCCTGTGGAAATGAAGTTGGGCACATTAGTAGAAAGCAGGACGA TAAATGTGTCCAATCTGTACGTCGGGGGAATTCCAGAGGGAGAGGGGACGTCACTGCTCA CAATGAGAAGATCGTTCCATGGCTGTATCAAAAACCTGATCTTCAATTTGGAACTTTTGG ATTTCAACAGTGCAGTTGGCCATGAGCAAGTCGACCTGGACACCTGCTGGCTGTCAGAAA GGCCTAAGCTGGCTCCCGATGCAGAGGACAGCAAGCTCTTGCCAGAGCCCCGGGCTTTTC CAGAACAGTGTGTGGTGGATGCAGCTCTGGAGTACGTTCCCGGCGCTCACCAGTTTGGTC TCACACAAAACAGCCATTTCATCTTGCCCTTTAATCAGTCGGCTGTCAGAAAAGAGCTCT CGGTTGAGCTAAGCATCCGCACGTTCGCCTCCAGCGGNCCTGATTACTACATGGCTCATC AGAACCAAGCAGACTACGCTGTGCTCCAGCTGCACGGGGGCCGCCTNCACTTCATGTTTG ACCTTNGGCAAGCAGAACAAGGTCTCTCACCTGCACTGCTCANTGATGGCCAGTGCACAC GTCAAGACNGACTATGTAAAGAAAAGCTNCATACTGTCGACGCCGAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091949 unedited NNGGGTTTGCTCCTGTTTTGGTACTTTTTTAAAAGCTCAAATGNAAAAACATAAAATACT ATCAAGTAATCAACAGAACATTCAATGTGTATAAAGATTTTTTTAAAAATACGTTTAAAA AGAGAGCCAGGGAATTCAATTTACATTTTAGACCATTTAATGGAGGTATTTGTTGCACAT GTGGTTTTAGTGTAACGTAAACACAACATCTCTCCCCAGAAACACTTAACCTGAGTTGGA AATGAAATATGAACTGAAGAGATTCTTAATTCACACATACACTTCTCCTCAAAATATTAG CAATGATTCCAACTGAGGATTCTGCTTGAAGTTCAGGACTCGGTCCCAGGACAGGAATGA AGGAAAACTCCGTGCAGTTCGAACGCTCTGCTGAAGTCAAAGGACTGCACCTGCGGGCTC TTAATCAGAGCTAGCTTCCTCAAACACCCGCGGAACGAGGTCTGGCTGCGCAGGCATTTT TGCTTCACACCAGCAGGATAGCCACCAACATAAATGGGATTGTTGGTGTCCACTGAGGTA GACTGGGTGTGTGGACTTTCAGCGCCAACTGCGTTCCCGTCAACAATCAGAGTGATACGG TGTTTGCTTTTGTTAGCTTGAAGAGTGTGCCATTTTCCATCACAGAGCACAGTGGCGGTT TTGGGCTCATATGCAGCTGTTATCCTGCCAGCACCATTGTTGACATGGAACAAGACCTTG CCGTCCACAAGCTCTAGTCCAATGGCATCCACTTTGGCAGTGCTGATCCCCAGGAGGACG CCATTCTGCGAGGAGGTTCGAAACTCCAGTGTGATGTTCACATCTGACTGGACTTGTAGC CCTCTTTTGACAGAGCTGCCTATCCGCTTCCGTCAAAGTATGGTTCCTTCCTGGGCCACT GCGTTACAC
Restriction Sites	NotI-NotI
ACCN	AK091949
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091949.1, BAG52448.1
RefSeq Size	2825 bp
RefSeq ORF	1587 bp
Locus ID	284217
Domains	LamG
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
Gene Summary	This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]

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