PNPO (AK026905) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026905, the custom clone sequence may differ by one or more nucleotides TATATATAGAGCACCTGGCACAGTGCCCGGCAGAGGGTGGGTTTGTAGATTCCCTCTGTAACTTCTCAGC CTTGTTTGCACACTGGCTTTTTTGTCCTGCTGGCTTCCTGGCCTCTGCTTCGTGGCCTTGCGACTTCATG CTTAAGCACGGAGCAGGGTTGGAAGGAAGAAACATGGAGTAGTGGCCCCTGACTGGAAGCTTCTTCGGAC AGGTGTACAACCCTTCCAGAGCCCCTGTAGTTGCTGCCACTGTCACTGCTGCTGCTGCAACCTCAAGATA AGGATCACACTGCCCAAGTTCAGCCTGCTGTCTGGGTGTCAGCCTGGAGCCCAGGCAGCACGGTGGCCAT TCATTGCTGCTTGTCAGAGAAGAATGCAGCTATCTTCTTTCCTGCGTGTGCTGGCAGCCGTCTGGGTGGC ATTAGTAACCCAGCCACCCCGTGGGCCTTCTCCACTTCAGCTCAGGTCTTTGCTGAGAGCCTGAGTTGTA GACGGAGGCTGTGAATGCGGGCTTGGTGAAGTGGGCTGAGCACGCCAGCAGATGGGTGGGCGATGGGCTT CAGCAGTGTCCCACTGCTGACTGTCAGTCTGGGAGTGGTGGCGTCCCCTTCTTCCCTCTGCCTCACAGAA GTTGCCTCCTGAAGTTGCCGCCTTAGTCTGAAACTGGGGGGTGCAGGGGTGTAGGGGTAGCGGGCAGCTT TGCAGAGGAGAGGTTAGGCAGGCAGGCAAAGCTTGATGGGTTTTGTGTTTTGCAGAGCACCGTGGCCTCC TGGGATGCTAAGGTCACCCTCAGGTCATCCCAGGTGTTTGCTCATGGCAACTCGCTTCCCTGGCGGTTGT CAAAGCCCCTCCAGGCCCATGCTTGTCTTCACCAAGGCCTTTCTTTCTCCTTCAGAGGCCCCTGGGAGCA GCTCCTGAGCTGGTGCTGAGGAGCCTCAAGGTACAAGATGGGAAAGAAATTGGAGGCCACAGGCTGGACA CCTGATTGGAACAAGAGCTATAGCCTGAGCGTCCAGGTGTCCAGCCAAGTTCCCAACCCATCTTCCCTGA GCCGCTTAAATTTACATTGTTCTCTTGCTCCCTGGAGGAATGGCAAGTTTTCTGTTTTCCTCTCCCTACA TGGATCCATATCTTCCTATAGCCACACAGAAGGTGCCAAGTAAATGTTTGTTGAATGAATGACTGACCTC TGGACAAGAGGTTTTCCTGCTTCCCCATTGATTCCAGCTGATCTCTGGGCTTCTTTCCATGGCTACCGAA GGAAGAGTAAAGTTTCTCTTCAAGCAGCCGTGCCTCTGGGCTCTCAGCTGTGCCCATCTTTCTCTTCCCC ACAGGGAGGATGTCCCATCCCAGAGCCTCCCTGGGCCCCTGCCTCTGCTGGCCCATACGTGTGTGGGCTG GGATTCTGTCCTCCTGTCCTTGTCCTCATCTGCTCCCTATGGTTTTGCTCCTTCTTCCATCCCCCCACTC ATCTGGGACCCTCCAGCCACTAAGAGACTCCACAGCAGCCCTAGGCCAGCTCTAGCCTAGTGTCTTCCCT TTGCTAGGTCCCCACTCTCCTGCATGAGGCGTCCACGCCAGGCACTGTCTTCACTGTGTAATGTCACCCA CTCCATCTTAGGGATGCTTGGGTCATATTTTGAAGGGGGGGTGTTTGAGACCTCTCCCTTCTCTCTTCCC CCATCTCACCTCCACGCCTTCGGAGAGAGAAGTGATCATGTGACCGCGGAAACAGGGATCAGAAAGGAAA TCAAATAACAGGAATTCCATCCTGGACACTGGGGCCTGACAAAGAGCTCTTGGACCAGTGCTGGATGCAA TTTGGGCGGTTTGGTTTGAATGGGGGAAATATGAGTTTCCAGAACAGGGTATTTGAAATCATGGCTACTC AGAAAATTGAGGCAGTGGTCACTCTGGCTGTAAATGCGGCACTCTGTGATTGTCAAGACCTTTGTAATTG AGGGTGCCTTGGCTGGGTCCAGGATATACTTCATCATAAGCCATATCTGGAGCCAGCATGAATTACAGGG GACAGGAATTCCCATTCATCGGTCACTTCCCACATGGGGCTAGGGATTTCGTGTGTACACTCATTCCATC TTCTCACGTGGCTCTGTGAAGTAGGTTTTGATATTCCCTTTTTACAGATGAGAGAGTGGAGACTCTGAAA AGTTAAATAACTGGCCCAGATTTAGTTAGTAAACAGCAGAGGTGGACTTTGACCCGTTGCTCTCACTGGC CCCAAAGCCTGTGTTCATGTTACACACTGGTCCCCTCCCACTCCAGGTGTCTGTACTTTTTGTGTCACCT TTGAGAAAGGTGGTCTTTTAGTTTCTTTAGCCACACGGTGAGCAGCTTGGACTCTGGGGATACACTAAAC TTGCCAGCTCTCTTCAATCCTCACATCCTGTGTTTCATTGCTAGTGTCCCTCCAGGATGGATATTCCAGT CCTCGCAGCTCAGGGCTCTGCACTCCCCATGAAAGAAGCATAACAATTAGCACCAAAAGCAAGCTACTGG GGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAAATCACACCATTG CTCTCTAACCTGGGAGACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026905 unedited TGTAATACGACTTACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGTAGCGGGCATCT TTGCAGAGGAGAGGTTAGGCAGGCAGGCAAAGCTTGATGGGTTTTGTGTTTTGCAGAGCA CCGTGGCCTCCTGGGATGCTAAGGTCACCCTCAGGTCATCCCAGGTGTTTGCTCATGGCA ACTCGCTTCCCTGGCGGTTGTCAAAGCCCCTCCAGGCCCATGCTTGTCTTCACCAAGGCC TTTCTTTCTCCTTCAGAGGCCCCTGGGAGCAGCTCCTGAGCTGGTGCTGAGGAGCCTCAA GGTACAAGATGGGAAAGAAATTGGAGGCCACAGGCTGGACACCTGATTGGAACAAGAGCT ATAGCCTGAGCGTCCAGGTGTCCAGCCAAGTTCCCAACCCATCTTCCCTGAGCCGCTTAA ATTTACATTGTTCTCTTGCTCCCTGGAGGAATGGCAAGTTTTCTGTTTTCCTCTCCCTAC ATGGATCCATATCTTCCTATAGCCACACAGAAGGTGCCAAGTAAATGTTTGTTGAATGAA TGACTGACCTCTGGACAAGAGGTTTTCCTGCTTCCCCATTGATTCCAGCTGATATCTGGG CTTCTTTCCATGGCTACCGAAGGAAGAGTAAAGTTTCTCTTCAAGCAGCCGTGCCTCTGG GCTCTCAGCTGTGCCCATCTTTCTCTTCCCACAGGGAGGATGTCCCATCCCAGAGCCTCC CTGGGGCCCTGCCTCTGCTGCCCATACGTGTGTCGCCTGCGATCCTGCCTCCTGTCCTTG NCCCATCTGCTCCTATGGGTTTGCTTCTTCTTCCATCCCCCCTNATCTGGTACCCTCCAC CACTAAGAACTCCACGCAGCCCTAGCCAGTCTACCCTATGTCTTCCTTTGCTAGGTCCCA CTTCTGCTTGAGCGTCACCCAGGCTGCTTATTTGGTATGCACCCTCATTTGGATGCG
Restriction Sites	NotI-NotI
ACCN	AK026905
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026905.1
RefSeq Size	2654 bp
RefSeq ORF	2654 bp
Locus ID	55163
Protein Pathways	Metabolic pathways, Vitamin B6 metabolism
Gene Summary	The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]

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