FBLIM1 (AK090827) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090827, the custom clone sequence may differ by one or more nucleotides GTGTAAACATCTCTATGGGGTGATGCATGCAGATAAATGTTTATGTGAACATATAAAGTCTGCATGTGTG GGTGAACACTCCACATCAGGTCATTGTCCATCATTACATATCTGTGGTGTCTGGCTCATTCGTCTCGTTT TCTGTCATTTTACCTACTTTTACCTGAAGTACAATGACTATTTAATAACTATTAAATGTTTCATTTCAGC TACACCTCTATGGTATTTTTTTCACCTCATATCATACCTTCCTCCCTCACTGGACTTCAGTGTGGGACCT GCAGGTCTCAGGCCTTCTGGGAAGTCCCAGCTTTTCTTGAATGTTTTCTCTCTCTGGCCAGCTCAGGCTG GAGCAGTGGGTGTTGGTGCCATCCTGTGGCTATAGGTGATAAGCCGGCTGTTCTTCACTGACCTGGTCTT ACCTTGAACCCTGGAGTTCTCACATGCATGGATTTAAATGCTGTGTCTTTCTAAGGGCACATTATGGCCA TTTTCCTTATCTCCCTCCCAAGGGGCTGTTTGCTGTGTTTATTGACTCCCTGTTTAAAAAACATATATAT ATATATATATATAGACGAATTAGGTTGTACCCCATAAATTGCATGTTTGAAAAATATTTCAAAGAATGGT TTATTTTTTTTTGCTGATTTTTATGTATCTTCTAAATTGTATATATTTATGGGGTATAAAATGATGTTGT GATTTTTCAATACAGTGTGGAATGAGAATCAAATTAACCTATCCGTTACCTCAAGTACTTGACTTTTTTT GTGATGAGAAGGCTGTTTATCACTTTCACTCACCAGCTCCTGCTGTCTTCCATGCTGGGGACAGGAAAGG CTCCACGCTTGACTTTCCCTCCTGTTTTCTTATCTGCTGTCAACCCCTGAATGTACCCTCCAGGATTGCC TTCTAGACCTTCTACATCTAGCTCGATCATTGTGCACCCACCTTCCTCCCAGGGACTGTCCTTAGGGAGT GACTTTATGCACAAACTTCACATGCTCTTCCTGCCTCCTTCATCCCCATAAGCCCCCATCTCAGCCCCAT CTGATTATTTTCCCTGTCTCATTTCCTGTCATGCTGTTGGTTTTCTAACCTGAAGGGTAACTTCTTACTC CTTAGTTTTTTGCCTTAATTTCTAAGTCATAGGCTACTTTCAGGCTAAAAGTTTTCCTCCAGCCATGGTT TTAGTCTCATTCTATAAGTCTTGCTAGGTAGTTTATTCATTGGAAATTTTTTGTTTTTGAGACAGAGTCT CGCTCTGTCACCCAGGCTGTAGTACAGTGGTGCCATCTCAGCTCACTGCAACCTCCGGTTCCCGGGCTCA AGCAGTTCTCTTTCCTCAGCCTCCTGAGTAGCTGGGATTATGGGTGCCTGCCACCACACCCAGCTAATTT TTGTATTTTTAGTAGAGACGGCATTTCACCATGTCAGCCAGGTTGGTCTCGAACTCTTGACCTCAAGTGA TCCGCCCACCTTGGCCTCCCAAAATACTGGGATTACAGGGGTGAGTCACCACACCCCACCCCTTGGCACT ATATTTAAGTGATGATATTTTTGGACACTGAGGTAGCCTCATTCCACAGGTTTAGATCTGAAGCATTTTC ACTATTGCTTCTTTCCATGTATTTCCTGGTTTGCAGGTCAGTTTCTTCTTTTAATTCATCATTGACACAG TGGTTTATATGGCTTTTAATTTCAAAAGATTTTTTAAAATATCTTTTTGCCGATTTTTATCTATGTTAAT TTTAACTAGACATGTTCCCTGGAAGGACATCACTAGTTTTTTTTTTTTTTTTTTTTTCACTCTGTTTATA ATTTAGGTGGATTTTCACATCTCAAGCCAGCTAAGAGTATGGGAAATAGGACTCGTTAAAGCACAGATAT CACACAAGTGATTCAGATTCACGAGGGTAATCCAGATGAAGGTATATCATTGTACCTGGCCCGCATCACT CAGTAACTGTCACCTTTGATTTATTGATTTACTTGAGATGGAGTTTGGTTCTGTCCCCGAGGCCGGAGTG CAATGGCGTGATCTCTGCTCACTGCAACCTCTGCCTCCCAGGTTCAGAGTGATTCTCCTGCCTCAGCCTC TTTAGCTGGGATTACAGGCACCCACCAACATGCCCAGCTAATTTTTGTATTTTTAGTAGAGAGGAGCTTT CACCATAATGGCCAGGCTGGTCTTGAACCCCAAATGATCTGCCCACCTTGGCCTCCCAAAGTGCTAGGAT TACAGGCTGGAGCTACAGAGCCCAGCCTGTCACCTTGATTTAAATGAATGCAGCTTTCTTGGTGTCTTGA TGTTTGTGAATTTCTGTGGAGTTTGATACTGCTCCTTTTGCTGCTTGTTTTTCCAGAGTCCCCTTATCAC TTGACGTGCTGTTATATTATGTTTGATTTATTACAGATACCCCATCAGCCTGAGTCTCTTAAAGGCCTTT ATACACATATGTCTACACATAGGGATTTGGATGATCTCGGGATCCCACATCCTCGCTGTCCCCTGTCCCC CCGCAACATCCCCCACCAATACCTTTCTGAAGTTTTCTAGTCCCTCCTTTTTGTTTGTGCTCCTTAAAGC CCAGCCCCATGCCTGACTTTGGTTCCCAGTGAGCATTGTACATTTGTGGATATTAAATCTTTGGCAAAGT CATTTACCTGGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090827 unedited AATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGACTTCACA GTGTCAGTGCAGTTTGTGTAAACATCTCTATGGGGTGATGCATGCAGATAAATGTTTATG TGAACATATAAAGTCTGCATGTGTGGGTGAACACTCCACATCAGGTCATTGTCCATCATT ACATATCTGTGGTGTCTGGCTCATTCGTCTCGTTTTCTGTCATTTTACCTACTTTTACCT GAAGTACAATGACTATTTAATAACTATTAAATGTTTCATTTCAGCTACACCTCTATGGTA TTTTTTTCACCTCATATCATACCTTCCTCCCTCACTGGACTTCAGTGTGGGACCTGCAGG TCTCAGGCCTTCTGGGAAGTCCCAGCTTTTCTTGAATGTTCTCTCTCTCTGGCCAGCTCA GGCTGGAGCAGTGGGTGTTGGTGCCATCCTGTGGCTATAGGTGATAAGCCGGCTGTTCTT CACTGACCTGGTCTTACCTTGAACCCTGGAGTTCTCACATGCATGGATTTAAATGCTGTG TCTTTCTAAGGGCACATTATGGCCATTTTCCTTATCTCCCTCCCAAGGGGCTGTTTGCTG TGTTTATTGACTCCCTGTTTAAAAAACATATATATATATATATATATAGACGAATTAGGT TGTACCCCATAAATTGCATGTTTGAAAAATATTTCAAAGAATAGTTTATTTTTTTTTGCT GATTTTTATGTATCTTCTAAATTGTATATATTTATGGGGTATAAAATGATGTTGTGATTC TTCAATACAGTGTGGAATGAGAATCAAATTAACCTATCCGTTACCTCCAGTACTTGACTT TTTTTGTGATGAGAAGCTGNTTATCACTTTCACTCACCAACTCCTGCTGTCTTTCATGCT TGNGACAGNAAAAGCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090827 unedited TATGAACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TCCACCCCAGGTAAATGACTTTGCCAAAAATTTAATATCCACAAATGTACAATGCTCACT GGGAACCAAAGTCAGGCATGGGGCTGGGCTTTAAGGAGCACAAACAAAAAGGAGGGACTA GAAAACTTCAAAAAGGTATTGGTGGGGGATGTTGCAGGGGGACAGGGGACAGCGAGGATG TGGGATCCCGAGATCATCCAAATCCCTATGTGTAGACATATGTGTATAAAGGCCTTTAAA AGACTCAGGCTGATGGGGTATCTGTAATAAATCAAACATAATATAACAGCACGTCAAGTG ATAAGGGGACTCTGGAAAAACAAGCAGCAAAAGGAGCAGTATCAAACTCCACAGAAATTC ACAAACATCAAGACACCAAGAAAGCTGCATTCATTTAAATCAAGGTGACAGGCTGGGCTC TGTAGCTCCAGCCTGTAATCCTAGCACTTTGGGAGGCCAAGGTGGGCAGATCATTTGAGG TTCAAGACCAGCCTGGCCATTATGGTGAAAGCTCCTCTCTACTAAAAATACAAAAATTAG CTGGGCATGTTGGTGGGTGCCTGTAATCCCAGCTAAAGAGGCTGAGGCAGGAGAATCACT CTGAACCTGGGAGGCAGAGGTTGTAGTGAGCAGAGATCACGCCATTGCACTCCGGCCTCG GGGACAGAACCAAACTCCATCTCAAGTAAATCAATAAATCAAAGGTGACAGTTACTGAGT GATGCCGGCCANGTACAATGATATACCTTCATCTGGATTACCCTCGTGAATCTGAATCAC TTGTGTGATATCTGTGNCTTAACGAGTCCCTATTTCCCATACTCTAGCTGGCTTGAGATG TGAAAATCCCCCTNATTATAAACAGAGTGGAAAAAAAAAAAAAAACTAGTGATGTCCTTC CAGGGACATGTNTTGTTTAAATTACATAGATAAAAAATCGCAAAAGAATT
Restriction Sites	NotI-NotI
ACCN	AK090827
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK090827.1
RefSeq Size	2672 bp
RefSeq ORF	2672 bp
Locus ID	54751
Gene Summary	This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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