GSDMA (AK096262) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096262, the custom clone sequence may differ by one or more nucleotides GCAGAACTTTCTAGAGACGGCGGGACTGAGCCGGTTCGGGCTCAGTGCTGCGGGCGCTTCGGAATTTTTT TTTCTTTTTTTTTTTTTGGTTCTTGCCTCCTTCTGGCTGCCTGCCCCCTCCCCCTTCTCCTGGGACCCTC GGACTCGGGATCTCCCCGGCGGCCTCCCTTGGCCCTTCCTCCCTCCCTCCCATCCCCGCCCACTTCCCTG TCTAACCGGATTCTTCCAGATTCTTCTCGATCGATCTCCCGACATCACTCCACCAGTCCGCTGCGCCTGC GGCGGGGTGTGGGGAGCGCAGGCTGCGGGATGGCAGTGGCGGCGCCCCCTCCCCGGGACCGGCCGTCACC ACGGGCGGCGTGGCCTCTCTGCTATGACCCGCGTAGACCCTTCCCTACTTGGGGGAAAGAGGGCAAGGGC GTGCGCGGGGAGCGGGGAGTGAGTGGACAGCGCTGCCCCGCTGCAGAGAGCCGGGGAAAGCGACCTCGAT TTCCCCAGATCCGAGAAGCCCCGGCCAGGCAGCGATGCCTCGCTCAGCGCCTGCAGATGCTGCGAGCAGC GGGGTTGGCAAGGCATGGCCCGCCCAGCTCGGAACTCAGATGGGGCACACACTCAGGGGACCCTTCCCGG CGGTAATAAAACTGCCGTTTCCGCGAATGCTTACAAAGATGGGTTCCCCTCACGAAAAAAGTCACTCGGT TTTCCTAAAGGCCCACAGAAGGAGTATTTTCTTCTCAATGTTTGCTTCCCGAGTGTTTTTCGACAGGCTG TGGAGCGCGCAGGTGCCGGTGTCACCTAAGCTATGGTCGTGGGACCTAGAGAGCCATCTAGAGAGGGGCC TGGTTTTGCTCAGCACCTAACCCCAGTTTAGCATAGTGCCTGGTGAGTGATGTCCCAATAGTTACTGGCT GCATGAATTTTCCTTTTTTAAATTTCTTTTTCCAAGTCTTGGCTGATTTTTTTTTCTTTTTTTTTTTTTT TTGAGACAGGGTCTTGCTCTGTCGCCCAGGCTGCAGTGCGGTGGTGCGATCTTGGCTCTCTGTAGCCGGG ACTACAGGCGTCCGCCACCACGCCGACTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGC CCAGGCTGGTCTCGAACATCTGGTCTCAAATGATCCGCCCGCCTCGGCCTCCCAAAGTTCTCGGATTACA GGCGTGAGCCACTGCGCCCAGCCTGAATTTTTATTCCAATAATAACTGTATTTTTGGACTTCCCGTTATG TGCCCGGCACTGTGCGATTGGACGCCATTAGTCTCATCAAACTAAATTGAAAGCTTTTTAGAGATGAACT TTCAGCGTCATAATGTGATGATTTATTGTAGTCCCTGCCGATGGAACTTGAAAGGGATGCAGAAGAAAAA GCCCTTCGCTTCAGCAATTTACAGCCCAAATGAGTTGAGCCATAATGAAGACGTCTCTTTAGGTGGCTGG AGACAGCTTCAGAGGATACGGGGCGCTGCCTCTGTAGGGGTTAAATCCACTACTTTTATGCAGAAGTAAC AGTGCCAGGGGTCACCCAAACTCCGGGAGACCAGGATGGTGGGTGCTGGCTTCCGGGGTGGCAGTTTACC GCAAACTGTCCTTGGCTTGCACCTACTGATTCTCCACGTGTGGCGGTTTCTTTTCTGCGTCTTTTTCTTC ATCGGAAGCGGACAGTGTGGGACCCCCCCTTTAATTCTGTTCGGCACGTACATAGTGGAGCCCTTCAGAT TGCCTGCCGTTACATTAACACATAGCCTAATACCCTAGTGGAATGAGCATGTGCTTTGGAGTAAGGGACG TTGGGCGGCCCATGTGTTATGTTAGCTGTTTAAATTGGGGCAGGTTAGTTAATCTTCATGTCTCGATTTC TTGTCTGTAAAATAGAGATAGTAACATTTGTAAAAGAGGGTGATTTAATGGTTAAAAAAGCGGGAATCTG TGTATGGTATCTAGCTCAGTCCTCGCCTCCAACTCCGCAGCTGGTAAATAACGGTCGTTTTAGTGTGATC TGATAGGCGCTGCAGTGGAGATGGGGACAAAGGGCTAAGAAAACAGAGGTCTGTGAGACAGGGAAGGGCA AGAGGGCAAGGTTCCCGGTGAGAAGCTCTGTCCCCACACAGTGAAGGAGGAAACTAGGGGACCACAGGCA GAAACTAGGAGAAACCACAGGAGGAAACTAGGGGTCTTGAATCCTGGAGCAGGAACTTACTGTTTCTCTT AGAATATTTCCTGGTCGTTTATGTGATATTTTTCTGATCATAAAAAGTACATATACTGT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096262 unedited NGTTCAGAATTGTATACGACTCACTATAGGCGCGCCGCGAATTCGCACGCGGGGCAGTGG CGGCGCCCCCTCCCCGGGACCGGGCGTGGGACGGGCGGCGTGGCCTCTCTGCTATGACCC GCGTAGACCCTTCCCTACTTGGGGGAAAGAGGGCAAGGGCGTGCGCGGGGAGCGGGGAGT GAGTGGACAGCGCTGCCCCGCTGCAGAGAGCCGGGGAAAGCGACCTCGATTTCCCCAGAT CCGAGAAGCCCCGGCCAGGCAGCGATGCCTCGCTCAGCGCCTGCAGATGCTGCGAGCAGC GGGGTTGGCAAGGCATGGCCCGCCCAGCTCGGAACTCAGATGGGGCACACACTCAGGGGA CCCTTCCCGGCGGTAATAAAACTGCCGTTTCCGCGAATGCTTACAAAGATGGGTTCCCCT CACGAAAAAAGTCACTCGGTTTTCCTAAAGGCCCACAGAAGGAGTATTTTCTTCTCAATG TTTGCTTCCCGAGTGTTTTTCGACAGGCTGTGGAGCGCGCAGGTGCCGGTGTCACCTAAG CTATGGTCGTGGGACCTAGAGAGCCATCTAGAGAGGGGCCTGGTTTTGCTCAGCACCTAA CCCCAGTTTAGCATAGTGCCTGGTGAGTGATGTCCCAATAGTTACTGGCTGCATGAATTT TCCTTTTTTAAATTTCTTTTTCCAAGTCTTGGCTGATTTTTTTTTCTTTTTTTTTTTTTT TTGAGACAGGGTCTTGCTCTGTCGCCCANGCTGCAGTGCGGTGGTGCGATCTTGGCTCTC TGTAGCCGGGACTACANGCGTCCGCCACCACGCCGACTAATTTTTGTATTTTTAGTAGAG ACGGGGTTTCGCCATGTTGCCCAGGCTGGTCTCGACATCTGGTCTCAAATGATCCGGCCG CCTCCGCCTNCCAAAGTTCTCGATACAGGCGTGGAGCACTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096262 unedited ACTATGNAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGCATTACA TTTTTGACAAATTATACTGGGAACATTCTAACTTAGGCTGGGTGACAGATTTCCAGCTTT CATTGTACTGGTATACTGCATAACATGTATGCATACATGAACACACACACCTATATGCAT TTCAGTCTTTTGAACGATCCAAATATCATACAATTATTTGGATGTTAAGTGCATTGGCAG CCTAGGAAGGTCAAGATATGAACTGTAGAAACAAGGGGTCTTCATGTGTAAATGATGGGC CCAGAGTTTGGGATCTGAGCCAGGTCAGCTCTAAGAGGCTCAGTGTGACGAAAACAAAGT TTCTGATCCCAGCAGGAGTCACCTACAGATAAGGTGGATGAGAAAAAGTGTGCTGACTTC CTCCCGCAAAAAAAGGGGACATTTTGGAAGAATTTCGAGTGGTACCGGTTACACATCTTT AATGAGAATACTAACTTAAATGGCCTGGGGAAAGGGTGAGGAGATAGAGGAAAGAGACTA AAACACGTGCACACTTTCTACAGTATATGTACTTTTTATGATCAGAAAAATATCACATAA ACGACCAGGAAATATTCTAAGAGAAACAGTAAGTTCCTGCTCCAGGATTCAAGACCCCTA GTTTCCTCCTGTGGTTTCTCCTAGTTTCTGCCTGTGGTCCCCTAGTTTCCTCCTTCACTG TGTGGGGACAGAGCTTCTCACCGGGAACCTTGCCCTCTTGCCCTTCCCTGTCTCACGACC TCTGTTTTCTTAGCCCTTTGTCCCCATCTTCACTGCAGCGCCTATCAGATCACACTAAAC GACCGTTATTTTACAGCTGCCGAGTTGGAAGCGNAGACTGAGCTAGATACATACCCAGAT TCCNGCTTTTTTAACATTAATCACCCTCTTTACAATGTACTATCTCTATTTACGACAGAA ATCAGACTN
Restriction Sites	NotI-NotI
ACCN	AK096262
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK096262.1
RefSeq Size	2299 bp
RefSeq ORF	2299 bp
Locus ID	284110
Gene Summary	May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).[UniProtKB/Swiss-Prot Function]

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