NECTIN1 (AK056455) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD111; CLPED1; ED4; HIgR; HV1S; HVEC; nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056455, the custom clone sequence may differ by one or more nucleotides TGCCCAAGGCCCACCCGTCTATTTCCACCTCATTCCCCCTTTAAGCTGAGAATGGGGTCTGCCTATAGAA GCTTCCAGAGACAGCCGAGACTGGGTCCCCGGTGCCTCCACCCTAGGATCTCAGGCCTGCCCCAAACTGC TTCTTACAGCGTGGAGGTTTCTCTGATTTCTCCCTTATCTAGCAACACTTGGTTCTTTTGGCTAGTCAGG GCTGGGATCCCAGGGGGATCCACCTGCAGGAGTCAGAGGTAAAGAAGCATCCACACGGAGGGTAGTGGGG AAGGAAGCCCACATGGCAGAAGCTCTGCAAACACAGTCCGAGGGACTGAGGAGTCCATACAATGCCTCAC ACACAAAGGCCATTGTCAAAGCCTCGGGCCTGGCAGAAAGTGAGGGGGGTGGGGCCGCTAGGCCACCTCC TGCTCAGCCACCCCTGGTCCTCATTCTGGGATGAGGGGAGGGCCTAGGAGAAGCCCTCCCTTTCAGTGCA GATTCCCTTCTTGAATGGGACATTGAGTTCCTATCAGCCACCCCAGACTGGGTCTTCCCATGTATATAAT GGAGGAAGACCCCTCCCAGCCCTCTCAGTCCCCCAGCTCAAAGTGCCTCAGTTCCTCCATCTGCTCCCAG ATCCCCTGGCACTCTAGACAGGCATGCCCATGGGAAGGACCCTGGGATGGAGAGGCAGGCTTCGCGGCCC TTCATGCCTGCCCACTGGGATTCCAGTTAACTCACCCCAGGGTGTCAGGGGCTCTGCAGGATGGCAAGTG TTGGGGAGGAGGGAGGCAGACCACTCTGTGCCCCACGGGCACTGACCCACCCTGCCATAGGCTTCCTAAG GGAGCCCCCCAACCCCTCCCCTCCCCTCCCCTCCCTGGTGCTGCTATTGAACCCCCTGCCAGCTCCATTT CTGCCCTGGCAACCCTGGGCCCAGCAGGTGGGTCGTCGTTGTCTGGCCCTCACTGCCCTGGCCCCAGCCC AGGCTGCCCAGTAGCCACGTAGCAGAAGTCTGAAGCCATGCCAGCCCTGGTGCGGCTCTCCCCTCTACAC ATTTGGGGTACTGGATAGGGTGAAGTTAGGGGAGAAAATTGTGGAGTCTTTCCAGCCACAGGGCAGAGGG AGGTGGAGGAGCTGGGCTGCTTTTCCCAGCAGTATTAGTGTCCCCCAGGACAGGGGACCTTTTCCACATT ACATCACTGCCCCATCCCACCTTACAACACTCTGGCCCCTCTGCTTGGTCCCCTTTTCCCCCAGGCAGGA GGCAATCCCAGGGGCCTGCCTGATAGAGGCATTTCCTGTCCCTGTCTCCTCCTGCATCTCCTTTATCCTG CACTGCCACCCTCTATTCCCCATTCTGTGTTGGACTTTGAAGGCCCCAAGCCCAGCCAAAGCACTGAGTT CCCCCTTAAGACACCTCCACACCCTCCCCACAAGCAAAGCACAAATTTTGGGGTCCATGTAGCATGGGCC ACGTAGGAGGCTCCTGACTTGCCAGGGGCCCAGCCTCAGCATACCCACCGAGCAGCTGCCAGCCTGGGCT GAGGGTGGGCATGAGGCAGGAGCCAGCACTTGGACCTAGGGATGTGAGGTTTTCTGTGCCCCAAGTTTGT GGGAAGGTGGGCACTACTGCTGGGCCCACAGACACAGCCAGCTGGCAAAAGGGAGGTCTAGCCCAGCAGA GAGATGAGGACATTTTGCTTCTCCTTCATGCCCACAGCATGAGCTGAGCTTCTGCTTTGCTGGAAATGAA ATAAACTTGGTATGAATTGTGCCAAGGCCTCCCCAGTTGTCATCCTGCCTCTTGTTGCCCTCCCTGTCCT TGCCCCCCACCCCACACCTATGCCCCTGTTTCCTTACAGATTTTGATATTGTTCTAATGTGTAATAGAAC CAGCCGAGTCCCCTTTTATCAGAAGGGTCTGAAAAGCAGCAGCACAGAGTAGGTGAACACAGGCCTGCAA GTGCGACCACCTCAGACCCAGTACGTGTGCCCACAGTGGACACACTCACACCTCCAACACACCCACGCGC AGGCATGTGTACACGCATGTACACACGCATGCATGCACAGCCAGATGGCCACTCAGCACAGATGTGGCAG AGGGAATGGTCTGATCCTGCTGAAAGCCATTAAGGAGAAACGAATTTCCCAGTGCCCGGGCTGCAAGAGA GCCTTATAGGGGCCCTGTTTCCTGGGCATGCGCTTCCTCTGCCAGCTAACCCCCACTTGCCCAAGTCACT GGTGCAATAACTTTTCTGCCTTCCTCAGAGCAGAGAAATTGGGAATTGTGTTAGGTGGGTGTGGGCAGCT CTGCTGAGCCAAGCAGACACGGATGTCCCCTCTTCTGGGAGGAGGGTAGTGCTCCCAGGCCTCAGGAGTC CAGACAGAGACCCCCAAAGCCTGACTGCCAACAGAAACCCTCTCCTAGTGAGGGGCAGGTGGGTGTGCCC CAGGTCCCCACACCCACAGGGAGGCTTCACACACTGCCCAGTACCGGGGATGCCAGGAGGCAGGCCCCTC TGCTGCTGCCACTGCTGCCAACACTGCCCAGCTTGTGAGGCCAGGAGGAGCCCCTGTCCCACTCGGTGCT GCTGCTCTTCTGACCCCTGCTGTGAGGAATGGGATTCTTGGTCGAAAAAATTGGTTTTCCTTTTTTGTAT AAATGAAAAGAATCCAGGAGAAGCTGCCACCCTCCCCTCCCAGCGTGATGCGCTACCTTGCTTCGGCGTC TTGTCGCCCTTTCCGCCTTTGGTCCAGGGACAGCCCAGCAGATCCTCCTGGTTCTGACCTGGGGGGTGTT TGCATCACCCCCTTTTACTTGTATTAAAAAAAAATGATGGGTTGAAAATGTACTGAGGATTAAAAATGTA CTTTTTTATAAATAAAGTGTTTAAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056455 unedited TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGTAAAGA AGCATCCACACGGAGGGTAGTGGGGAAGGAAGCCCACATGGCAGAAGCTCTGCAAACACA GTCCGAGGGACTGAGGAGTCCATACAATGCCTCACACACAAAGGCCATTGTCAAAGCCTC GGGCCTGGCAGAAAGTGAGGGGGGTGGGGCCGCTAGGCCACCTCCTGCTCAGCCACCCCT GGTCCTCATTCTGGGATGAGGGGAGGGCCTAGGAGAAGCCCTCCCTTTCAGTGCAGATTC CCTTCTTGAATGGGACATTGAGTTCCTATCAGCCACCCCAGACTGGGTCTTCCCATGTAT ATAATGGAGGAAGACCCCTCCCAGCCCTCTCAGTCCCCCAGCTCAAAGTGCCTCAGTTCC TCCATCTGCTCCCAGATCCCCTGGCACTCTAGACAGGCATGCCCATGGGAAGGACCCTGG GATGGAGAGGCAGGCTTCGCGGCCCTTCATGCCTGCCCACTGGGATTCCAGTTAACTCAC CCCAGGGTGTCAGGGGCTCTGCAGGATGGAAGTGTTGGGGAGGAAGGAGGCAGACCACTC TGTGCCCCACGGNCACTGACCCACCCTGCCATANGCTTCTAAAGGGGAGCCCCCCCAACC CCTTCCCTTCCCTTCCTGGTGCTGCTATTGAACCCCCTGCCAGCTCCATTTCTGCCCTGG CAACCCTGGGCCCAGCCAGTGGGTCGTCGTTGTCTGGCCCTCACTGCCCTGGCCCCAGCC CAGGCTGNCCAGTAGCCACGTAGCAGAAGTCTGAGCCATGCCAGCCCTGGTGCGGCTCTC CCTCTACACATTTGGGTACTGNATAGGGTGAAGTTAGGGGAGAAAATTGTGGAGTCTTTT CACCCCAAGGCANAGGGAGGTGGAGGAGCTGGGCTGCTTTTTCCACAGATTAAGTGTCCC CAGAAAAGGGCACCTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056455 unedited GTCCCTCTATGNACGCGGCCGCAATCTANGTCGAGTTTTTTTTTTTTTTTTTTGTTTTAA ACACTTTATTTATAAAAAATACATTTTTAATCCTCAGTACATTTTCAACCCATCATTTTT TTTTAATACAAGTAAAAGGGGGTGATGCAAACACCCCCCAGGTCAGAACCAGGAGGATCT GCTGGGCTGTCCCTGGACCAAAGGCGGAAAGGGCGACAAGACGCCGAAGCAAGGTAGCGC ATCACGCTGGGAGGGGAGGGTGGCAGCTTCTCCTGGATTCTTTTCATTTATACAAAAAAG GAAAACCAATTTTTTCGACCAAGAATCCCATTCCTCACAGCAGGGGTCAGAAGAGCAGCA GCACCGAGTGGGACAGGGGCTCCTCCTGGCCTCACAAGCTGGGCAGTGTTGGCAGCAGTG GCAGCAGCAGAGGGGCCTGCCTCCTGGCATCCCCGGTACTGGGCAGTGTGTGAAGCCTCC CTGTGGGTGTGGGGACCTGGGGCACACCCACCTGCCCCTCACTAGGAGAGGGTTTCTGTT GGCAGTCAAGCTTTGGGGGTCTCTGTCTGGACTCCTGAGGCCTGGGAGCACTACCCTCCT CCCAGAAGAGGGGACATCCGTGTCTGCTTGGCTCANCAAAGCTGCCCACACCCACCTAAC ACAATTCCCAATTTCTCTGCNTCTGAGAANGCAGAAAAGTTATTGCACCAGTGACTTGGG CAAGTGGGGGTTGGCTGGCANNAGAAGCGCATGCCCAGGAAACAGGCCCCTATAGGCTCT CTTGCAGCCCGGGCACTGGGAAATCGTTTCTCCTTATGGGCTTCACCAGATCAGACATTC CCTCTGCCCATCTGTGCTGAGTGGCCATCTGGCTGTGCATGCCTGCGTGTGTACATGC
Restriction Sites	NotI-NotI
ACCN	AK056455
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056455.1
RefSeq Size	2897 bp
RefSeq ORF	2897 bp
Locus ID	84853

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