ADCY5 (AK098381) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AC5; FDFM
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098381, the custom clone sequence may differ by one or more nucleotides GTGTTTCTGAGAAAAATATTTATTAAATAAAACAAAACAAGTTCTCCGTGCCCTTCTTTAGACTATGCTA GTTGTATGCGTGTAAGAGACACACAAGCAAACGAGGACGCCACTCGGGGGGAGGGCGGGGATCCCCACTT GTCTTTTTTGTATTTTTTATTTTGTATTATTGAAAGCCTTGGAGATCTCACAGATAGATATGCCAAATTC TATATTTTGTAAATTCTCTATATTAGAAAACAGCTGTGCACAGCAGGGCGGGTGTGCTCATTTGTACTGT GTGTATGTCGGTGTATGTACTGGTGTATATGTGTGTGTGTTCATGCTGTGGAACTGGTCTCACACAGGAT GTGTTTCCCTCATTTCAGATTTGGCAGTTTTGGGTTTTCCAAGGTACCACCAGAGCAGTGGGTGTGTGCT TTTGGGGTACCATATGCTCAGATTAAGTAGGAGGATGCATGGACACACTGCCCCATCTTTTCTGACACAC GCACACGTATGTACACACATGCACACACCCTCCTTCCCCTAAGCAAAACGCAGATGGAATAAGAAAACAA AAAGCTGCTTTCCCATCCCAGGCCGAGCTGGAACCAAGGGAAGCAATCTCATCCTGCGACAGGCAGTGTG GTGCCCTCCACACCCTGAGATTTCAGACGTTTGCGGCTTACAGAGGCAGCGCCCACAGATTCCAGAGTGC TTACAGAAGGCCAGGTGCTTTGCAGGCTGGGACGAGGAAGCCAAGCCTCCCTGGCCTACTCAGTTGGCCA AGGTGCAGGTGGCTCTTCCTGGAGATGTTCACTCAGACTGGGGGATGCAATGTGCAGCCTTCAGGTTTGC GGAAAGGGAGTGGCCTTGACCTCCACCGGCAAACCAGGCAGAGGAATGGGTAGAGCCCAGCTTTAGAGTC CACAGGGAAAGCTAGCAGGAATTTTGTTTTAGTGGGAGGGGGCAGTTAAACATACCAAGAAAAAAATACT ATTTTTATAACCTATGAGGAAGACATTTGGAAAATGATACTCTAGCACAGAATTCAGTGGAATCCTTAGG GCCCATGCCCAAATCTTTCCATTGCTTCTCAGGTTAGAATGATCTTCACCTCCAACATGAGCTTGGAGGT GATGAGGCAGTGGCTCTGTGCCAGCTGCCACAATGTGACTTTGATGTCCACCTGTACCACCTCTCACTGG GCTCTAGCACCACCCTCCCCTCCCCGCACACCAACTGAACACAGCTCTGAGAAGCAAAGTGTGTGGACCC AAAACTGCCAAGCCTGAGTCTGTCCCGTGCTTCTGCTGCTCCATCCTTTGAGTTCTGCATTGCCATCCTG ACGTCGGCCACAGGAGGCCTGCTTTCTTCCAGCTGTTGTTCTCAAGTTCCCTGCCCCTCCACATCGCCCG CCAGTGGTGTTGGGTTTTCGTTCTGCTTCCAACCTGAGTAAAGTGTGTGTGCTGAGTTCATCCCATGTTC TCCCATGGTCATGGCTTCCCGGCCCCATGGGGACCCCTCTCCCATCCCAGCAGTGACTGGTGACAGTGTG CAGGTGCAGTGCTAGCTCTTCGTTCCCTCTAAAGGGTGTGCACTCTTTTTATTCCTACTCTTGCAAAAAC AGATACGATTATGATTTCCCATGGAAATTGAAAAGTCTATTTAAATAATTTAACTATTAAACACTTTCAC TGGT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098381 unedited NGGTTCAGATTAGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACAGACA GTGCCCTAGGAGGGAAACAGAACTAATTACGAGGGGGAGGCAAGAGGACGCCAAGCAAGG AGTGGTGATTCTGAGAAAAATATTTATTAAATAAAACAAAACAAGTTCTCCGTGCCCTTC TTTAGACTATGCTAGTTGTATGCGTGTAAGAGACACACAAGCAAACGAGGACGCCACTCG GGGGGAGGGCGGGGATCCCCACTTGTCTTTTTTGTATTTTTTATTTTGTATTATTGAAAG CCTTGGAGATCTCACAGATAGATATGCCAAATTCTATATTTTGTAAATTCTCTATATTAG AAAACAGCTGTGCACAGCAGGGCGGGTGTGCTCATTTGTACTGTGTGTATGTCGGTGTAT GTACTGGTGTATATGTGTGTGTGTTCATGCTGTGGAACTGGTCTCACACAGGATGTGTTT CCCTCATTTCAGATTTGGCAGTTTTGGGTTTTCCAAGGTACCACCAGAGCAGTGGGTGTG TGCTTTTGGGGTACCATATGCTCAGATTAAGTAGGAGGATGCATGGACACACTGCCCCAT CTTTTCTGACACACGCACACGTATGTACACACATGCACACACCCTCCTTTCCCTAAGCAA AACGCAGATGGAAATAAGAAAACAAAAAGCTGCTTTCCCATCCCAGGCCGAGCTGGAACC AAGGGAAGCAATCTCATCCTGCGACAGGCAGTGTGGTGCCCTCCACACCCTGAGATTTCA GACGTTTGCGGCTTACAGAGGCAGCGCCCACAGATTCCAGAGTGCTTACAGAAAGCCANG TGCTTTGCAGGCTGGGACGAAGAAGCCAAGCTNCCTGCCTACTCATTGGCCAGGTGCANG TGGCTCTTCTGAGATGTCACTCGACTGTGGATGCATGTGCACN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK098381 unedited GTACAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCGGGAAAGGGTTTAATAGTTAAATTAT TTAAATAGACTTTTCAATTTCCATGGGAAAACATAATCGTATCTGTTTTTGCAAGAGTAG GAATAAAAAGAGTGCCCCCCCTTTAAAGGGAACCAAAAGCTAGCCCTGGCCCTGCACACT GTCACCAATCACTGGTGGGATGGGAAAGGGGTCCCCATGGGGCCGGGAAGCCCTGACCCT GGGAAAACATGGGATGAACTCAGCACACCCCCTTTACTCAGGTTGGAAGCAAAACGAAAA CCCCACCCCCCTGGCGGGCGATGTGGAGGGGCAGGGAACTTGAAAACAACAGCTGGAAAA AAGCAGGCCTCCTGTGGCCAACGTCAGGGTGGCAATGCCAAACTCAAAGGATGGAGCAGC AAAAGCACGGGACAAACTCAGGCTTGGCAGTTTTGGGTCCACACACTTTGCTTCTCAAAA CTGTGTTCAATTTGTGTGCCGGGAGGGGAGGGTGGTGCTAAAACCCAATGAGAGGTGGTA CAGGTGGACATCAAAGTCACATTGTGGCAGCTGGCACAGAGCCACTGGCTCATCACCTCC AAGCTCATGTTGGAGGTGAAGATCATTCTAACCTGAGAAGCAATGGAAAGATTTGGGCAT GGGCCCTAAGGGATCCACTGAATTCTGTGCTAGAGTATCATTTTCCAAATGTCCTTCCTC AAGGGTATAAAAAAAGGAATTTTTTCTTGGGATGGTTAACTGCCCCCTCCCACTAAACCA AATTCTGCTAGCTTTCCCCGGGGACTCTAAAGCTGGGCTCTACCCCATCTCTGCCCGGGT TGCCGGNTGAAGGTAAGGCCCCTCCCTTTTCGCAAAT
Restriction Sites	NotI-NotI
ACCN	AK098381
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK098381.1
RefSeq Size	1684 bp
RefSeq ORF	1684 bp
Locus ID	111
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
Gene Summary	This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

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