H6PD (AK024548) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CORTRD1; G6PDH; GDH
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK024548, the custom clone sequence may differ by one or more nucleotides CTTTAGCCCTACTCTTGGGCCCCCAAGTTAGCAGAGTAATCAAAGCTTCCTACCGTTTGGCCTACTATTC CAGACTAGTCCCTCGAGGGGTTCCCTTCCAAAATATGCAGGGCTCAGGCTCCCAATTCCGGGCCTGTCTG CTTTGCTTGTGTTTCTCCTGTCCCTGTTCTCCCGGAGGGCCCAGGTGGAACTCACGACAGGGAGGGAGAC GCTTCCCAAAAACCTGCAGGGCTATTTCCCAGAATTTGGTTTTCAAGTACAAAACTTTTTGTCCTGTAAG ATATATGCAGCCTCACAGAAGCAGCCTCTGCCTCCACTTTACCAGCTACGTTTTTATCTTAAGCACATGG GGCTCCCTTAGAACTTACTCCACTGATTTAAAAAAAAAAAACTGCCTGGCAGCATCTCAGTGTCAGAGTG AGCACGGCACAGGAAAGGCCCGTGGTGACGAGGGTGAGGTGGCCACAGTGACCGGACGACAAATGAGACT CTGCAAATGAGACTCCAGAGGGTGAAGATCTGCGGTCTCCAGACATCATAGGCCATGTGACCCACTAGGG GCCGCTTACCCCTGGCCGTCCGCTGGCTGAACTGAACGCATTCCCTCTCTCCGCAACTCTCCCGTGAGGC TGCACCCGTGTGGGTAGCACTGGAAGCGGCACTGTTTGCATTGTACATAGGAAGGAAGGAAGTTCTTCCA GCCTCACCAGCACCTGGCAGCGAGTCAGAGCCTGTGAGGGCATCCGAAGCAGTGATGCAGTGTCAACCTC CCAGCTGGTGCCACTCTGCCCTCGGGGGCTCCAAGCATTGTAACTCAGTCATGGGAGCTGCCTCTTTGGA AGTGCAGATTTATTCCTGTAATAATCCTGCCTGCTTTTACCTCTCGTCCACTGACCAGCAAGTGTGAGTC CCGGTGTCAGTCGGCACAGTCCAGTGTCCATCTGCATTTGCTCATGCAGAGGGGGTGAGTTGGGCACTCC CTGTTGTTGGTTTTCCTTTTGCAGCACACTGGGCAGTCTCCCTATAAAACAAAAACCCCACCTTCTGTGC CTTCTGCTTTAGAGCAGAGCTCCCCCTCCCATTTCCTCAGTCTTCCCTGCAAAATCTGTCCACCGGGGAA GGCAGCAGGAACCCTGGGCAGCGGGTGTTCTGGGAAGGCTAGTGACAGCAGATGTCATCCAGGAACAGCC ACACACGGTTCTCCAGGCCGCCGTCAGCAGCTCAAGGTGGGGTATGAGTGAGAAGCTGAGGATCTCGCAG CTTGTTGCTGAGCAAGGTGCAACCGGGCTCATGCTGTCATCAGCACAAGACGGGATGGCAAGGGCTTTCA GACGCATTTCCAAGAGTCCAGCAAGCCAGGGGGAAGATGATCCCTTTGCCGAAGTGTACCCTCTAGCCAA CTTTTGGGAGCGCTTCTGTTTGCAAAGCGCTGGGGATGTGCCTGTCTCTGTGTGACCCACGAACGGGAAG GGAGAGCACTGGAGTAATGACACTTCTGCTGCTGCTTTGATTCTCAAGGCTGATCTTTAAAACCCTCGCC TTGCTGACAGGTGCTTTAAAGGCAGTCTGCATCTTTTCTTCCCTTGGTGTGGGAGAGGTAAACACTTTGA TTTGCTGAAAGCTGTATGGAGTATATTTGAACAGCTAGTAGTTAGCTTTGAAAGTGGAAGTGTGAACAGA CACTACTTGTGTCGCTTTGGGTCCTTCACTTTACCCCCACAGAAGTCTAGAGGCGTCTGTTATAAAGCGT TACGGGGCGCCTGCATGCAGGAGGAAGGACCTGTATTAGCTGGAAATCATCAGGAACCCAGCTTGCCTCC ATCTCTCTGAGATGTGCTGGGTACAGCCTGCCCCTCCTAGTTCTGTCCACCGGGAAGAGCCGGCTGGCGG CAGATCCCCAGGGGCAGAGCCCCTGCTGGATCCTGGGAGCTCATCTTTACCTGTGCCGGAGTGGGAACTG TGATTCCAGCCGGGCAGGTCAGAGTGGAGCAGTGCTAAGAGGCTGTTGCAGGAGAGCTAGACGGGCGGGG CCTGCTGCATCTGGATCATGTTTCTGTGCTCTGCCCCGCGCTAGGGACTCAGGGTCTGGGCTTCTGCCAG GTGAGGAGCAGAGAGACTGTTCCCTTGGGTGGAGAGGTGTGGGCATGAGAGCCACCCATTGCCAAGCAGC AAGAATGTTCGTGCTTTTTTCCAGAGAGGGGAACCCCACTGGTTTTTGTGGAAACAATGGAAACTTACAG ATGCCTGCCTGGGATGATGAGGCACATTCAGAACAAATGCTTTTTTTTTTTTGAGACAGAGTCTCGCTCT GACGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAAACTTTGCCTCCCAGGTTCAAGTGA TTCTCCTACCTCAGCCTCCCGAGTAGCTGGGATTACACCACCATGCCCAGCAAATTTTTGTGTTTTTAGT AGAGACGGAGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCATCCGCCTTG GCCTCCCAAAGTGCTGGGATTACAGGCGGGAGCCACCATGCCTGGCCAGAACAAATGCCTTTTTAAACCT TTTAAGAACATTTTTAAAATGTCTTTTTCTATGTCAAATGTAACGTTTATTTTTTTAAACAATAAAATTG ATTTGCCAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK024548 unedited AGGAATTTGTACTACGATCTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCCACAGTC CAGGTTCCCTGGTGGGGACCAATGATTCCATCCGCATGGAAGCCCACGTGTGCACTTAGG GGCCCATAAATGGCAGAAGGGCCCCTCCTTTGGGAGACCTTGTCAGTCAGCATCTCTAGG GCAACCGTGATTGCCATTTGTAGAGGGGAAGGAATCAAGGGACTTTAAGCTAGATCAAAA TCTGGGGACAAATTCTCCTGCTAACTGCAAGTTAAAATAGGCCCTTCTTACTGAATTTCC CTGTTTGTTTCTCTGCAGACAATGCTTTAGCCCTACTCTTGGGCCCCCAAGTTAGCAGAG TAATCAAAGCTTCCTACCGTTTGGCCTACTATTCCAGACTAGTCCTTCGAGGGGTTCCCT TCCAAAATATGCAGGGCTCAGGCTCCCAATTCCGGGCCTGTCTGCTTTGCTTGTGTTTCT CCTGTCCCTGTTCTCCCGGAGGGCCCAGGTGGAACTCACGACAGGGAGGGAGACGCTTCC CAAAAACCTGCAGGGCTATTTCCCAGAATTTGGTTTTCAAGTACAAAACTTTTTGTCCTG TAAGATATATGCAGCCTCACAGAAGCAGCCTCTGCCTCCACTTTACCAGCTACGTTTTTA TCTTAAGCACATGGGGCTCCCTTAGAACTTACTCCACTGATTTTAAAAAAAAAACTGCCT GGCAGCATCTCAGTGTCAGAGTGAGCACGGACAGGAAAGGCCCGTGGTGACCAGGTTGGA AGTGGCCACAGTGACCGGACGACAAATGAGACTCTGCAATGAGACTCAGAGGGTGAAGAT TTGGGGTCTCCAGACTCATAGCCATGTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK024548 unedited GGTCCATCATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTGGCAAATCAATTTTATTGTTTAAAAAAAAAACGTTACATTTGACATAGAAAA AAACATTTTAAAAATGTTCTTAAAAGGTTTAAAAAGGCATTTGTTCTGGCCAGGCATGGT GGCTCCCGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGATGGATCACCTGAGGTCA GGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAACACAAAAA TTTGCTGGGCATGGTGGTGTAATCCCAGCTACTCGGGAGGCTGAGGTAGGAGAATCACTT GAACCTGGGAGGCAAAGTTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGC GTCAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAGCATTTGTTCTGAATGTGCCTCATCA TCCCAGGCAGGCATCTGTAAGTTTCCATTGTTTCCACAAAAACCAGTGGGGTTCCCCTCT CTGGAAAAAAGCACGAACATTCTTGCTGCTTGGCAATGGGTGGCTCTCATGCCCACACCT CTCCACCCAAGGGAACAGTCTCTCTGCTCCTCACCTGGCAGAAGCCCAGACCCTGAGTCC CTAGCGCGGGGCAGAGCACAGAAACATGATCCAGATGCAGCAGGCCCCGCCCGTCTAGTT TTCCTGCGACAGCCTCTTAACACTGCTCCACTCTGACCTGCCCCGCTGGGATCACAGTTC CCACTCCGGCACACGTTAAGATGAGCTCCCAGGATCCAACAGGGGCTCTGCCCCTGGGGA TCTTGCCGCCGCCCGCTCTTTCCGGTGGACAGAACTAGGAAGGGCAAGCTTGTCCCAGCC ATTTTAAAGAATGGAGGCCAGCC
Restriction Sites	NotI-NotI
ACCN	AK024548
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK024548.1
RefSeq Size	2684 bp
RefSeq ORF	2684 bp
Locus ID	9563
Protein Pathways	Metabolic pathways, Pentose phosphate pathway
Gene Summary	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]

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