ASAH1 (AK025211) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AC; ACDase; ASAH; PHP; PHP32; SMAPME
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK025211, the custom clone sequence may differ by one or more nucleotides ATGTTGATAAACATTTTGGATGTGATGTTGGAGAACAATTGCAACCTGCCCAGATGTTCAGCTCGCCCGA AGGCTGCCTCCTTGGTGACTTGTTTCATCTTGAGTCTAGAGAGTCCAGGCTGGGGAAGAAAACCACCTCC AGCCGGCCGTTCCAGCACAGCCCCACTGGCCAAAGCACACACTCACACAATGAAAACACTGAAACTTTTT CTTAAAACAAATTTTTCTCCCATTGTCATGGTTCTGTATATTAACACAAAATTTTCTTTTCTCATAGGAG AGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAGCAGAAGACAAAAA AGGTAATTGTGCGCTGAGGGAGGGTGGGCAGCATGAAGCACTACATAAAGAAAAATCTTCTAAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK025211 unedited AGGTTCAGATTAGGNATACCATCATATAGGCGGCCGCGNAATTCGGCACGAGGAAAGTTT TATCTTTAAGCTAGCAAAGCAGTAGTGTCATTATTTTAAATAGCCAACAGTACCTTCATT TTATGAGATGATTGAGAATTTGTGAAAATCAGTAGTATTTATCAACAAAAGTGGCTAGTT TTGTGTGCTGCTTCTTTAAAAAAATCATTTAAAAGATAGCATACTGGAAATATGTACTTG CTGCCTTTATGGAAAGCAAGTCTAATTCCTTTATTATGTAAAGAACTCTAGCAACTCAAT ACCAAATAGAAATACCTTATGGATTAAGAAGAATAACATGATGTATAAGTGGTGTAAATT TATATAATTATTATAAATTATTATAAATGTATAATAAAATAACTAGAATTTCACCAAATT AGAAATTTAATGGAAATAAAATACACATTGGATTTGGGTGAAAAAAACATGTTGAGCTTA ACATGTTAATGTTAAATGGGACAGAAATACAATGTGCTGCCTACTTTTGAATTAAATGCT AAATAGCAGTATTATACATTTTGGGAAAATATTCAAAAAATAAAATACCCTAGAATGGAG TTTCTTGGCAGCTCTGTACTAATTTTAATAGTTCTTGAATCTCCTTTTTCACCTTAGAAA TTTTAGTCACACTTTAAAAATCTGGGCAAAATGTATGTCCAGCAAACAGCCAAGAGCCAG ATGTATTAAATGATCATATTTACCCTCCCAAAAGAAAATTTAAAATGGAAGCCTGTTGAT AAACATTTTGCGATGTGATGTTTGGAAACCAATTGCAACCTGGCCCAGATGTTTAACTTC GCCCGCAAGCTGCCCTCCTTGTGGACTTGTTTCATTTTAGATCTAAAAAGCCCAGCCTGT GGAAAGAAAACACCTCCACCCGGCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK025211 unedited CTTGCCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATGGAGTCTG GCTCTGTATCCCAAGCTGCAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCCGCCTC GCAGGTTCAAAAAAGTCTCTGCCTCAGGCTCCCAAGTAGCCGGGATTACAGATGCCCACC ACCATGCCTGGCTAATTTTTATGTTTTTAGTAGAGACGGGGTTTCACCATCTTGGCCAGG CTGGTCTTGAACTCCTGACCTCGTGATCCACCCGTGTCAGCCTCCCAAAAAGCTGGGATT ACAGGCGTGAACCACCATGCCTGACCCTTTGTTCTTTACTTACCCAAGAAATACTCCAAA ATCCATGTTTCTCCCATGTATTAGATGACCTATTTGAAGGTAGACAGAAGAGACGTGTAA TAGCAGTTAAGATTCTAAATCAAAGCTGGACAATTAGTTTTGATTAATTTATTTATTTTT GAGACAGAGTCTCGTTCTGTTGGCCAGACTGGAGTGCAGTGGTGCGATCTCAGCTCACTG CGACCTCTGCCTCCCAGGTTCAAGCAATTCTTGTGCCTCAGCCTTCCACGTAGTTGGGAT TACAGACATGTGCGACCGCACCTGGCTACTTTTTTTTTTTTTTTGTATTTTTCTTTTAGT AGAGAAGGGGTTTTACCATGTTGGCCAGTCTGGTGTCAAACTCCCAACCTCAGATGATTC GCCCGCCTCGGCCTCGCAAAGTGCTGGCATTACAGGTGTAAGCCACTGTGCCTGGGCCTC TAATTTGATTAATTTTATTCAAACTGTTTCTTCAAACCATAGAGTNAGAACATNCTATAT CCTAAGCTTCACANAGGGACTGTTGCACCATCTTCTGCAGATAACCACAAATCTGAAACC GGGGTTTCCAGTAGATCAATATTGGAAAGGATCATGGCATGGTTGGGTCCTATCCCG
Restriction Sites	NotI-NotI
ACCN	AK025211
Insert Size	2590 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK025211.1, BAB15085.1
RefSeq Size	1774 bp
RefSeq ORF	417 bp
Locus ID	427
Protein Families	Druggable Genome
Protein Pathways	Lysosome, Metabolic pathways, Sphingolipid metabolism
Gene Summary	This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]

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